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Diagnostic control system

An antibody and immunochromatographic technology, which can be used in material testing products, measuring devices, instruments, etc., and can solve problems such as less favorable

Inactive Publication Date: 2006-10-18
NORWEGIAN ANTIBODIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods necessitate more complex production lines for manufacturing control and test areas, which is less favorable

Method used

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  • Diagnostic control system
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0037] According to a preferred embodiment of the present invention, the antigen is selected from Staphylococcus aureus protein—protein A or protein G, or hemocyanin, preferably mollusk hemocyanin. Non-limiting examples of other suitable antigens according to the control system of the invention are avidin, preferably deglycosylated avidin or streptavidin.

[0038] Based on the specifications and descriptions of the present invention, those skilled in the art can understand that other substrates than the substrates specified here can be used as the substrates of the control system of the present invention.

[0039] In addition, the "antigen" can also be derived from its natural source, or can be prepared by methods known in the art. For example, where the antigen is a protein, such as a bacterial protein (such as protein A), the protein can be isolated and further purified from cell cultures, such as Staphylococcus aureus cultures . The antigen can also be produced by recombi...

Embodiment 1

[0070] Blue latex with immobilized anti-Protein A chicken antibody

[0071] IgY is isolated from hen eggs (or affinity purified with protein A immobilized in a gel column) by the conventional ammonium chloride precipitation method well-documented in the literature and well known to those skilled in the art. Hens have been immunized with protein A in suspension in Freund's complete adjuvant. Thirty milligrams of this antibody were dialyzed for 24 hours against 15 mM NaCl in 10 mM borate buffer, pH = 9.3, in both volumes adjusted to 15 mL in the same buffer.

[0072] Estapor Carboxylated microspheres PSI 90-21 with an average diameter of 0.117 and an equivalent weight of 164 carboxyl moieties per gram of latex particles were purchased from Merck. 90 mg of the particles were dialyzed against water for 12 hours. Water was added to bring the volume to 4.5 ml.

[0073] 5 mg of 1-ethyl-3(3-dimethylaminopropyl)phosphodimethylamine (abbreviated as EDC) was dissolved in 3 ml of water...

Embodiment 2

[0075] Example 2 Gold Colloid with Immobilized Anti-Protein A Chicken Antibody

[0076] Mix 10 ml of distilled water containing 1% gold chloride with 1 liter of boiling distilled water. 10 mL of a 34 mM sodium citrate solution adjusted to pH 4.2 was added and mixed for 20 minutes. Colloidal gold is formed. The suspension was cooled to room temperature. The pH was adjusted to 7.2 by adding 1 ml of 1% PEG (polyethylene glycol) 20.000. The size of the gold colloidal particles was determined by measuring the optical density at 540 nm and 600 nm using conventional techniques. The method can be adjusted so that the average particle size obtained is between 540 nm and 600 nm. The glassware used must be siliconized.

[0077] Using the method described by Slot and Geuze in Eur. J. Cell. Biol. 38:87-93, 1985, the gold colloidal particles were labeled with a chicken anti-Protein A antibody to the point of saturation according to the method. The labeled gold colloids were then suspe...

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Abstract

A novel control system applicable in immunochromatographic analysis is provided, wherein the control system comprises a binding pair consisting of an antigen and an antibody, and wherein at the antigen a species-specific substance, and further provided that the antigen is not an antibody per se.

Description

technical field [0001] The present invention relates to a control system for in vitro immunochromatographic analysis, which system comprises a binding pair consisting of an antigen and an antibody against said antigen, wherein said antigen and analyte are derived from different species, and the set The antigens themselves are not antibodies. [0002] In addition, another aspect of the present invention relates to a control system for use in an immunochromatographic system, the system comprising a binding pair, wherein one member of the binding pair is immobilized on a colored particle, wherein the color of the colored particle is the same as that in the The microparticles used in the immunochromatography system to develop the color of the analyte to be detected are different in color. The analyte is detected by the immunochromatography system. Background technique [0003] Immunochromatographic techniques have been used as the primary principle of many in vitro diagnostic t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N30/02
CPCG01N33/54388
Inventor 厄于斯泰因·拉森
Owner NORWEGIAN ANTIBODIES
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