Multi-channel column imaging fluorescent detector

A fluorescence detector, multi-channel technology, applied in the field of detectors, can solve the problems of light source intensity attenuation, limited quantitative detection, difficult to achieve, etc., and achieves the effect of reducing the size of the instrument, reducing the cost and volume, and being easy to implement.
CN1869662AInactive Publication Date: 2006-11-29TSINGHUA UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TSINGHUA UNIV
Publication Date
2006-11-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a multichannel column imaging fluorescence detector, comprising: excitation light source, microflow control chip, emitted light filter plate, high voltage power supply and computer control system, in turn arranged, and characterized in that: the excitation light source adopts small-molecular organic LED and plural electrodes of the light source is connected by external circuit so as to make it emit single linear light source or plural different-length linear light sources according to the requirements; the ultrathin excited light filter plate is arranged between the excitation light source and microflow control chip, lens and charge coupling detector are arranged on top of the emitted light filter plate. And the invention can conveniently implement column imaging detection mode without optical fiber, cylindrical prism and complex optical components, further reducing bulk and cost and effectively improving detection sensitivity.
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Description

technical field

[0001] The invention relates to a detector, in particular to a column imaging fluorescence detector which is used in an isoelectric focusing electrophoresis system of a microfluidic chip and adopts an organic light-emitting diode (OLED) as a light source and can realize multi-channel detection. Background technique

[0002] Protein is a kind of ampholyte molecule. In the pH environment greater than its isoelectric point, it will dissociate into negatively charged ions, and electrophoresis to the positive electrode in the electric field; while in the pH environment lower than its isoelectric point, it will dissociate into Positively charged ions swim toward the negative electrode until they are in a pH environment equal to their isoelectric point, that is, when the net charge is zero (ie isoelectric point, pI), they do not stop. At this time, the migration of charged molecules under the action of an electric field Equilibrium with diffusion movement. If this ...

Claims

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