Method for separating protein adopting superparamagnetism silicon nanometer granule static adsorption
A superparamagnetic and nanoparticle technology, which is applied in the application field of nanomaterials and nanobiology, can solve the problems of cumbersome pretreatment of ion exchange columns and non-specific adsorption of proteins, so as to improve the action speed and reduce the operation intensity. Effect
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[0035] Example 1: Extraction of cytochrome C from a mixed solution of bovine serum albumin (acidic protein, pI4.6) and cytochrome C (basic protein, pI10.6):
[0036] 1. Adjust the pH value of the mixed solution of cytochrome C and bovine serum albumin: adjust the pH value of the mixed protein solution to 8.0 with a 1 / 15mol / L disodium hydrogen phosphate solution at room temperature.
[0037] 2. Formation of superparamagnetic pure silicon shell nanoparticles-cytochrome C complex: Add 15mg of superparamagnetic pure silicon shell nanoparticles to 5.0mL of mixed protein solution, and mix well at room temperature of 15~25℃. Incubate for 0.50 hours to form a superparamagnetic pure silicon shell nanoparticle-cytochrome C complex.
[0038] 3. Separation and collection of superparamagnetic pure silicon shell nanoparticles-cytochrome C complex: using the superparamagnetism of superparamagnetic pure silicon shell nanoparticles, under the action of an external magnetic field, the superparamagn...
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[0042] Example 2: Cytochrome C in a mixed solution of bovine serum albumin (acidic protein, pI4.6), immunoglobulin G (basic protein, pI8.0) and cytochrome C (basic protein, pI10.6) Extraction of:
[0043] 1. Adjust the pH value of the mixed solution of bovine serum albumin, immunoglobulin G and cytochrome C: adjust the pH value of the mixed protein solution to 8.0 with a 1 / 15mol / L disodium hydrogen phosphate solution at room temperature.
[0044] 2. Formation of superparamagnetic pure silicon shell nanoparticles-cytochrome C complex: Add 3.0mg of superparamagnetic pure silicon shell nanoparticles to 1.0mL of mixed protein solution, mix well at room temperature of 15~25℃. Incubate for 0.50 hours to form a superparamagnetic pure silicon shell nanoparticle-cytochrome C complex.
[0045] 3. Separation and collection of superparamagnetic pure silicon shell nanoparticles-cytochrome C complex: using the superparamagnetism of superparamagnetic pure silicon shell nanoparticles, under the a...
Example Embodiment
[0049] Example 3: Extraction of bovine serum albumin from a mixed solution of bovine serum albumin (acidic protein, pI4.6) and cytochrome C (basic protein, pI10.6):
[0050] 1. Adjust the pH value of the mixed solution of cytochrome C and bovine serum albumin: at room temperature, use 1 / 15mol / L potassium dihydrogen phosphate solution to adjust the pH value of the mixed protein solution to 5.0.
[0051] 2. Formation of superparamagnetic aminoated silicon shell nanoparticles-bovine serum albumin complex: Add 9.0mg of superparamagnetic aminoated silicon shell nanoparticles to 3.0mL of mixed protein solution, at room temperature of 15~25℃, Mix well and incubate for 0.50 hours to form a superparamagnetic amino silica shell nanoparticle-bovine serum albumin complex.
[0052] 3. Separation and collection of superparamagnetic aminated silica shell nanoparticles-bovine serum albumin complex: use the superparamagnetic properties of superparamagnetic aminated silica shell nanoparticles to se...
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