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Method for fermenting and producing xanthan gum using steam-explosion straw as raw material

A technology of steam-exploding straw and xanthan gum, which is applied in the field of fermentation engineering, can solve the problem of high cost, and achieve the effects of improving utilization rate, strong accessibility, and low price

Inactive Publication Date: 2006-12-27
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Xanthan gum fermentation mainly uses raw materials such as sucrose, glucose and starch, and the cost is relatively high, so it is urgent to develop an alternative substrate to produce this product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: after the steam-exploded wheat straw is crushed to 60-80 meshes, tap water is added in a mass ratio of 1:4; the pH is adjusted to 4.5 with hydrochloric acid, and then cellulase is added at a ratio of 20 units of enzyme per gram of steam-exploded wheat straw and Stir evenly; hydrolyze at 45°C for 80 hours; press filter after enzymolysis, the obtained filtrate has a reducing sugar concentration of about 40-50g / l; add activated carbon accounting for 5% of the weight of the filtrate, boil and filter to obtain a clear hydrolyzate;

[0021] In the hydrolyzate after decolorization, add the NH that accounts for 4% of the mass of the hydrolyzate 4 NO 3 , 0.5% yeast extract, 2% light calcium carbonate and 0.3% Na 2 HPO 4 12H 2 O as a culture medium;

[0022] Insert Xanthomonas campestris 1.1781 in the medium, and the inoculum size is 1.4×10 per milliliter of medium. 8 cells; and at a temperature of 32°C, after fermentation for 72 hours, the xanthan gum was obtaine...

Embodiment 2

[0023] Embodiment 2: After the steam-exploded rice straw is pulverized to 60-80 mesh, tap water is added in a mass ratio of 1:9. Adjust the pH to 5.5 with sulfuric acid, then add cellulase at a ratio of 10 units per gram of steam-exploded wheat straw and stir evenly. Hydrolyze at 55°C for 40 hours; press filter after enzymatic hydrolysis, and obtain a filtrate with a reducing sugar concentration of about 40-50 g / l. Add and account for 15% active carbon of filtrate weight and boil and filter; Obtain clarified hydrolyzate;

[0024] Add 1% NH to the decolorized hydrolyzate 4 NO 3 , 0.2% yeast extract, 1% light calcium carbonate and 0.1% Na 2 HPO 4 12H 2 O as a culture medium;

[0025] Insert Xanthomonas campestris 1.1781 in the medium, and the inoculum size is 1.4×10 per milliliter of medium. 8 cells; and at a temperature of 25°C, after 96 hours of fermentation, ethanol precipitation or other methods were used to separate and extract xanthan gum.

Embodiment 3

[0026] Embodiment 3: After crushing the steam-exploded corn stalks to 60-80 meshes, tap water was added in a mass ratio of 1:7. Adjust the pH to 5.0 with phosphoric acid, then add cellulase at a rate of 40 units per gram of steam-exploded wheatgrass and stir evenly. Hydrolyze at 50°C for 60 hours; press filter after enzymolysis, and obtain a filtrate with a reducing sugar concentration of about 40-50 g / l. Add and account for 19% gac of filtrate weight and boil and filter; Obtain clarified hydrolyzate;

[0027] In the hydrolyzed solution after decolorization, add 3% NH that accounts for the mass of the hydrolyzed solution 4 NO 3 , 0.1% yeast extract, 1.5% light calcium carbonate and 0.2% Na 2 HPO 4 12H 2 O as a culture medium;

[0028] Insert Xanthomonas campestris 1.1781 in the medium, and the inoculum size is 1.4×10 per milliliter of medium. 8 cells; and at a temperature of 30°C, after 80 hours of fermentation, ethanol precipitation or other methods were used to separa...

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Abstract

The invention relates to a method for preparing xanthan gum by employing airing straw as raw material, comprising following steps: disintegrating airing straw, adding them into water, adjusting pH with acid to 4.5- 5.5, adding fiber enzyme according to ratio of 10-40 units of enzyme for each gram airing straw, stirring evenly, proceeding enzymolysis at 45-55 Deg. C for 40-80 hours; decoloring hydrolyzate; adding NH4NO3, yeast paste, light calcium carbonate and Na2HPO4*12H2O into decolored hydrolyzate as culture medium; inoculating Xanthomonas campestris 1.1781 for fermentation at 25-32 Deg. C for 72-96 hours, separating and getting xanthan gum. The invention is characterized by cheap raw material and low cost.

Description

technical field [0001] The invention relates to a method for producing xanthan gum in the field of fermentation engineering, in particular to a method for producing xanthan gum by fermenting steam-exploded straw as a raw material. Background technique [0002] Xanthan gum (Xanthan gum), also known as Xanthomonas polysaccharide, Xanthan gum, Xanthan gum, etc., is a general term for extracellular heteropolysaccharides produced by Xanthan bacteria. It is one of several microbial polysaccharides currently being developed at home and abroad. The most characteristic one is also the microbial polysaccharide with the largest production scale and widest application in the world. Because xanthan gum has good properties such as thickening, pseudoplastic rheology, water solubility, suspension, emulsification stability, acid and alkali resistance, salt resistance, temperature resistance, and excellent compatibility, its application coverage reaches more than 20 industry for 30-40 produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/06
Inventor 陈洪章张志国
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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