Alpha-galactosidase solid fermenting producing method

Abstract

The solid fermentation process of producing alpha-galactosidase includes the following steps: 1. preparing spore suspension; 2. preparing the first order seed; and 3. fermentation of alpha-galactosidase. Or, the solid fermentation process of producing alpha-galactosidase includes the following steps: 1. preparing spore suspension; 2. preparing the first order seed; 3. preparing the second order seed; and 4. fermentation of alpha-galactosidase. The alpha-galactosidase produced through the said process has high enzyme generating activity, simple preparation process and easy production application.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for producing alpha-galactosidase for feed by solid-state fermentation. Background technique

[0002] α-galactosidase is an oligosaccharide characterized by the specific hydrolysis of non-reducing terminal α-1,6-galactosidic linkages of galacto-oligosaccharides and polygalacto-(gluco)mannan Hydrolase has great economic value and application value. Since α-galactosidase can hydrolyze the polysaccharides of oligosaccharides and galactomannans such as melibiose, raffinose, stachyose and verbascose in beans, and can improve sugar metabolism, it is mainly used In feed and food industries.

[0003] At present, the feed industry is developing rapidly with the regionalization and scale of animal husbandry production and the increase in the proportion of feed, and the contradiction of shortage of feed resources in my country is becoming increasingly prominent; according to experts'...