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Broom corn millet drought-resisting regulating gene MYB transcription factor gene and application thereof

A technology for transcription factor and gene regulation, which is used in genetic engineering, plant genetic improvement, application, etc.

Inactive Publication Date: 2007-02-07
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has the characteristics of drought tolerance, stress resistance, and high water use efficiency. It is one of the most drought-resistant crops among cereal crops. It is an excellent genetic resource for improving plant drought resistance. After searching, no publications or reports on drought resistance and high water use efficiency of broomcorn millet were found. Relevant literature on cloning of related regulatory genes

Method used

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  • Broom corn millet drought-resisting regulating gene MYB transcription factor gene and application thereof
  • Broom corn millet drought-resisting regulating gene MYB transcription factor gene and application thereof
  • Broom corn millet drought-resisting regulating gene MYB transcription factor gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Connect the gene with the expression vector containing the corresponding plant promoter (such as CaMV 35, etc.), construct the expression vector of the broomcorn drought resistance regulatory gene MYB class transcription factor gene, transform Escherichia coli, etc. to obtain the bacterial strain containing the gene; or combine the gene with Contain the T-DNA binary carrier of corresponding plant promoter (such as CaMV 35 etc.) to connect, construct the T-DNA expression vector of broomcorn millet drought resistance regulation gene MYB class transcription factor gene, transform Escherichia coli etc. to obtain the bacterial strain that contains this gene, and Corresponding Agrobacterium strains are fused to obtain an Agrobacterium strain containing the gene.

Embodiment 2

[0038] The Escherichia coli strain or the Agrobacterium strain obtained in Example 1 are propagated, and the DNA of the expression vector containing the drought-resistant regulation gene MYB class transcription factor gene of broomcorn millet is extracted or directly utilize the bacterium liquid obtained by propagation, and adopt the well-known methods known to those skilled in the art. Methods (such as pollen tube passage method, gene gun method, Agrobacterium-mediated method, etc.) to transform crops such as wheat and corn or other plants; screen transgenic plants with proper expression of the gene, and improve the drought resistance and moisture content of existing crops or plant varieties usage efficiency.

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Abstract

the invention discloses a transcription factor gene of broom corn millet drought resisting moulding gene MYB and application, whose genome sequence is 1739bp with 121bp (347-467bp) and 93bp (599-691bp) as two intrones and three extrons, wherein the whole length of cDNA sequence is 1525bp with 212bp as 3' non-translating region and 41bp as 5' non-translating region; the coding region is 1272bp, which codes 424 amino acids; C-end contains a plenty of Ser. the RT-PCR expressive analysis of gene displays that the gene possesses certain expression under normal condition, which induces to express under dry condition (relative soil water content is 36%).

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering. In particular, it relates to a drought-resistant regulation gene MYB transcription factor gene derived from broomcorn millet, its encoded protein and its application in cultivating drought-resistant and water-efficient plants. Background technique [0002] At present, the research on the genetic improvement of crop drought resistance and water use efficiency is mainly by isolating the corresponding genes of protein synthases related to the biological water deficit response and its regulation from various organisms (including microorganisms, plants and animals). Proline synthase genes, trehalose synthase genes, transcription factors that transmit signals and regulate gene expression cloned from microorganisms, insects and plants have been successfully combined with different promoters into plants (such as sugar beet, tobacco, Arabidopsis thaliana, etc.) h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82
Inventor 胡银岗林凡云
Owner NORTHWEST A & F UNIV