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Chemical luminescent analysis reagent kid for prostate specific antigen

A prostate-specific, chemiluminescence technology, applied in the field of chemiluminescence immunoassay kits, can solve problems such as inappropriate capture, and achieve the effects of no radioactive pollution, short validity period, and easy operation

Inactive Publication Date: 2007-03-07
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because its luminescence is often flickering light, it is not suitable for capture, and it is often only suitable for automatic chemiluminescence immunoassay instruments

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035]Prostate-specific antigen chemiluminescent quantitative detection kit, consisting of (1) a 96-well opaque polystyrene plate coated with anti-prostate-specific antigen monoclonal antibody, (2) using the analysis buffer as a matrix, adding prostate-specific antigen The prostate-specific antigen series standard substance that pure product is prepared, (3) the antibody of the prostate-specific antigen labeled with horseradish peroxidase, its working concentration is 1: 8000, (4) luminescence substrate A liquid, ( 5) Composition of luminescent substrate solution B. The luminescent substrate A solution is made of high-efficiency luminescent agent, compound enhancer and amino acid, and the luminescent substrate B solution is made of amino acid oxidase and stabilizer.

[0036] Opaque polystyrene plates coated with anti-PSA antibodies were coated by direct physisorption.

[0037] The luminescent substrate A solution is composed of the following components: luminol 0.15mM, hydrox...

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Abstract

This invention relates to prostate gland specific antigen chemical specifying agent box in the clinical testing analysis technique field, which comprises the following parts: non-transparent polyphony lacetylene board with the gland antigen, prostate antigen series standard product, enzyme mark gland specific antigen, lighting bottom object A liquid composed of effective light agent, compound strengthening agent and amino acid and B liquid composed of amino acid oxidize and stabilizer. The lighting bottom A and B is of HRP luminal lighting system.

Description

technical field [0001] The invention belongs to the technical field of clinical blood immune detection, and in particular relates to a chemiluminescent immunoassay kit for quantitative detection of prostate-specific antigen (PSA) in human serum. The kit has strong detection specificity and high sensitivity. Background technique [0002] In recent years, with the aging population structure and changes in eating habits, the incidence of prostate diseases in my country has increased rapidly, among which prostate cancer accounts for the second in male incidence and the third in mortality, and the early detection of prostate cancer is the The key to whether it can be cured. Prostate-specific antigen (PSA) is the most specific tumor marker for prostate cancer. The detection of PSA is helpful for early detection of prostate cancer, monitoring of disease progression, and monitoring of treatment effects. Plays a decisive role in the differential diagnosis of prostate cancer. At pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/547G01N33/52G01N21/64G01N33/531
Inventor 付光宇李晓霞王睿许远航李彬马建军
Owner AUTOBIO DIAGNOSTICS CO LTD