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Strain capable of generating avermectin B component and utilization thereof

A technology of avermectin and strains, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of complex process, high cost, low extraction rate, etc.

Inactive Publication Date: 2007-03-14
郭正 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, as mentioned above, the abamectin produced by Streptomyces common avermitilis contains 8 components with similar structures, and to extract the B1a component with the highest activity and the lowest toxicity, industrial production must go through many organic solvents. Steps such as extraction, crystallization and column chromatography, the process is complicated, the cost is high, and the extraction rate is very low

Method used

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  • Strain capable of generating avermectin B component and utilization thereof
  • Strain capable of generating avermectin B component and utilization thereof
  • Strain capable of generating avermectin B component and utilization thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Medium preparation

[0025] No. 1 medium (strain medium):

[0026] A. Medium ratio (by weight percentage):

[0027] Glucose 1.5%, DL-asparagine 0.05%, beef extract 0.3%, potassium dihydrogen phosphate 0.05%, agar powder 2.5%, distilled water to make up to 100%, pH (before sterilization) 7.4.

[0028] B. Preparation

[0029] Weigh the glucose first, put it in a large beaker, add a little water to dissolve it (distilled water), then pick up the beef extract with a spoon and put it in a small 100ml beaker, weigh it with an electronic balance, add a small amount of distilled water to melt it, and pour it into the dextrose In a large beaker, stir well, then put DL-asparagine and potassium dihydrogen phosphate into a small beaker, weigh it, add a little distilled water, heat it on an electric stove with asbestos tiles, and pour into the above solution , Dilute to the total volume used, stir evenly with a glass rod and adjust pH=7.4 (before sterilization).

[0030] Weig...

Embodiment 2

[0043] Example 2: Mutagenesis and screening of strains producing abamectin B component

[0044] 1. Mutagenesis treatment

[0045] 1.1. UV mutagenesis treatment

[0046] Taking the existing avermectin-producing Streptomyces avermitilis GY115 as the starting strain, the avermectin produced by this strain contains 8 components (see Figure 1). First, fresh slant spores of GY115 are used to prepare a single spore suspension according to a method known to those skilled in the art, and 5 ml is sucked and placed in a sterile petri dish with a diameter of 60 mm. The plate was placed 20 cm under an ultraviolet lamp with a wavelength of 253.7 nm and a power of 30 W, and the lid was opened and shaken on a 7921 magnetic stirrer at a speed of 1370 revolutions / min for 30 seconds. When the time is up, transfer to the separation plate of No. 1 medium containing an appropriate amount of antibiotics (gentamicin 0.0004% w / v or chloramphenicol 0.0008% w / v or streptomycin 0.001% w / v), and place Incubat...

Embodiment 3

[0062] Example 3. Rejuvenation of strains

[0063] Select the type II single colony of X-28, which is mouse gray, large and full, and straw hat type. In the sterile room, use the inoculation loop to scrape the spores and place them in the aseptic operating procedure. In a test tube of sterile water, add glass beads, shake, and filter with absorbent cotton. Dilute 0.5ml bacterial suspension according to the multiple dilution method (add 4.5ml sterile water) and then dilute it 5 times. Use a 1ml pipette to drop 2 drops of the bacterial suspension on the plate containing No. 1 medium, spread it evenly with a glass spatula, and incubate at 28°C for 11-14 days. The isolated morphology is rat-grey, large and full, and the single colony of straw hat type was subjected to secondary titer verification.

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Abstract

The present invention relates to one kind of Streptomyces avermitilis generating abamectin B1a and abamectin B2a only. The Streptomyces avermitilis has the strain numbered as X-28 and has the preservation number of CGMCC No. 1765. The present invention also relates to the application of the Streptomyces avermitilis in producing abamectin.

Description

Invention field [0001] The present invention relates to a Streptomyces avermitilis (Streptomyces avermitilis) that produces only B1a and B2a in the B component of avermectin and a method for producing avermectin by using the strain. Background of the invention [0002] With the improvement of people's living standards and the call for green food, biological pesticides are very popular in the current pesticide market, and avermectin (AVM) is the most popular and has great development potential in the current biological pesticide market Biological pesticide products. AVM is a high-efficiency antibiotic developed in 1979 by Kitasato University in Japan and Merck in the United States. It increases the efficacy of antibiotics by 25 times than that of the same generation. The dosage is reduced from mg / kg to μg / kg. , And it is a new type of antibiotic that integrates pesticides, veterinary drugs and medicines. As a new type of biological pesticide, abamectin has the following unique adv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/62C12R1/465
Inventor 吴学民沈德堂杨玉淮储消和郭正
Owner 郭正
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