Beta-lactamase SHV gene variation detecting chip and application thereof

Abstract

The present invention discloses one kind of beta-lactamase SHV genovariation detecting chip. The beta-lactamase SHV genovariation detecting chip includes a solid phase carrier and a probe, which is made to hybridize with the nucleotide sequence of the beta-lactamase SHV gene to be detected and / or its complementary sequence. The present invention also discloses the method of using the chip in detecting beta-lactamase SHV genovariation. The chip of the present invention may be used in simultaneous detection of several kinds of beta-lactamase SHV genovariation and in detection of bacterial drug resistance and resisted drug kinds for directing reasonable drug use and avoiding abuse of antibiotics.

Description

technical field [0001] The invention relates to a bacterial drug resistance gene detection chip, in particular to a β-lactamase SHV gene variation detection chip and its application. Background technique [0002] Since the invention of penicillin in 1929, antibiotics have played a huge role in the prevention and treatment of bacterial diseases, greatly improving people's quality of life and prolonging people's life expectancy. However, due to the long-term and extensive use of antibiotics, especially the irregular abuse of antibiotics, the problem of bacterial resistance has become increasingly serious. The most important mechanism of bacterial drug resistance is the production of inactivating enzymes, such as β-lactamases and aminoglycoside inactivating enzymes. β-lactamase can covalently bind to the carboxyl group of the β-lactam ring, hydrolyze its amide bond, and inactivate penicillins and / or cephalosporins. β-lactamase is not only associated with high morbidity and mo...

AI Technical Summary

Problems solved by technology

[0007] In my country, most antibiotics are administered in the first way, but with the use of new antibiotics and the emergence of new drug-resistant strains, the feasibility and scientificity of choosing antibiotics based on experience has been decreasing day by day. If the empirical medication is wrong and then choose method three, it will not only delay the patient's timely and reasonable treatment, aggravate the condition, but also waste manpower and material resources; method two can only roughly divide antibiotics into β-lactam and non-β-lactam administration, It is impossible to judge whether the pathogenic bacteria have drug resistance, and it is not suitable for multiple infections; the third method is the best drug delivery method currently available, but because this method is time-consuming (3 days at the fastest) and laborious, the operation procedure is cumbersome. The experimental results are affected by a variety of uncertain factors and there are large errors. Repeated verification is often required, and the mechanism of drug resistance cannot be clarified. In addition, the drug sensitivity test is limited by the experimental antibiotics, so it cannot provide reasonable information in time for the first diagnosis. The use of antibiotics in the program can only retrospectively evaluate the correctness and error of the antibiotics used, and provide a plan for the adjustment of antibiotics

In addition, the third method itself also has defects: some conditional pathogenic bacteria are difficult to cultivate, and multiple infections are often missed

[0008] At present, the detection methods of β-lactamase commonly used in domestic hospitals include single disc method, double disc synergy method, circle estimation method, small disc diffusion method, agar dilution method and inhibitor enhanced diffusion method, etc. The detection accuracy, operability and detection cost have their own defects, and most of these methods stay in the traditional detection of microbial phenotypes, and fail to directly identify whether the chromosomal DNA or plasmid DNA of bacteria carries the encoding β -Lactamase genes, and early and accurate detection of different types of β-lactamase genes is of great significance to the clinical rational use of antibiotics and the prevention of nosocomial infection epidemics or wider epidemics

Images