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Deproteinizing process of gullan gum fermenting liquid

A fermentation broth and deproteinization technology is applied in the field of deproteinization of gellan gum fermentation broth to achieve the effects of easy amplification, simple process and low energy consumption

Inactive Publication Date: 2007-03-21
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, in the process of using Sphingomonas paucimobilis to ferment and produce microbial polysaccharide gellan gum, due to the special hydrodynamic properties of gellan gum, many engineering problems have been brought to its industrial production. is one of the most difficult problems to solve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The deproteinization method of gellan gum includes fermentation broth pretreatment, deacylation treatment, and enzymatic deproteinization treatment; wherein the process steps and process conditions in the deproteinization method are:

[0019] (1) Strain selection: select Sphingomonas paucimobilis XLJ CCTCC No.M206058;

[0020] (2) Slant culture: inoculate the above-mentioned bacterial strains on a solid slant medium, and cultivate them for 20 hours at 30°C;

[0021] (3) First-level seed cultivation: the bacteria cultivated in step (2) were placed in 100mL seed liquid medium with an inoculation loop under sterile conditions, and shaken for 10 hours at 30°C to obtain First-class seed liquid:

[0022] (4) Expanded cultivation: with an inoculum size of 5% by volume, connect the primary seed solution to 500 mL seed liquid culture medium, and culture under shaking for 8 hours at 30° C. to obtain the secondary seed solution;

[0023] (5) Fermentation tank cultivation: with t...

Embodiment 2

[0032] The deproteinization method of gellan gum includes fermentation broth pretreatment, deacylation treatment, and enzymatic deproteinization treatment; wherein the process steps and process conditions in the deproteinization method are:

[0033] (1) Strain selection: select Sphingomonas paucimobilis XLJ CCTCC No.M206058;

[0034] (2) Slant culture: inoculate the above-mentioned bacterial strains on a solid slant medium, and cultivate them for 24 hours at 25°C;

[0035] (3) First-level seed cultivation: the thallus cultivated in step (2) was put into 50mL seed liquid culture medium with an inoculation loop under sterile conditions, and shaken for 12 hours at 25°C to obtain first-class seed liquid;

[0036] (4) Expanded cultivation: with an inoculum size of 8% by volume, connect the primary seed solution to 500 mL seed liquid culture medium, and culture under shaking for 10 hours at 25° C. to obtain the secondary seed solution;

[0037] (5) Fermentation tank cultivation: w...

Embodiment 3

[0045] The deproteinization method of gellan gum includes fermentation broth pretreatment, deacylation treatment, and enzymatic deproteinization treatment; wherein the process steps and process conditions in the deproteinization method are:

[0046] (1) Strain selection: select Sphingomonas paucimobilis XLJ CCTCC No.M206058;

[0047] (2) Slant culture: inoculate the above-mentioned bacterial strains on a solid slant medium, and cultivate them for 18 hours at 33°C;

[0048] (3) First-level seed cultivation: put the bacteria cultured in step (2) into 100mL seed liquid culture medium with inoculation loops under aseptic conditions, and vibrate for 9 hours at 33°C to obtain first-class seed liquid;

[0049] (4) Expanded cultivation: with the inoculum size of 10% by volume, connect the primary seed liquid in 1000mL seed liquid culture medium, under the condition of 33°C, vibrate for 4 hours to obtain the secondary seed liquid;

[0050] (5) Fermentation tank culture: the volume ra...

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Abstract

The present invention discloses deproteinizing process of gullan gum fermenting liquid, and the process includes pre-treatment of the fermented liquid, deacylating treatment of the fermented liquid, deproteinizing enzymolysis treatment of the fermented liquid, and other steps. The present invention is used effectively in the post-treatment of the extracted microbial polysaccharide, and has the advantages of simple operation, mild condition, low power consumption, low material consumption, low extracting cost and other advantages.

Description

technical field [0001] The invention relates to a method for deproteinizing fermented liquid, in particular to a method for deproteinizing microbial polysaccharide gellan gum fermented liquid. Background technique [0002] Gellan gum is a new type of microbial polysaccharide produced by Sphingomonas paucimobilis, featuring reproducibility, good compatibility, thermal stability, wide pH range, acid resistance, enzymolysis resistance, low dosage and aroma release It has unique properties such as strong resistance, and has a wide range of uses in food, pharmaceutical, daily chemical and other industries. Because of its superior properties to gels such as xanthan gum, carrageenan and sodium alginate, it has become another hot spot in the research of microbial polysaccharides. [0003] However, in the process of using Sphingomonas paucimobilis to ferment and produce microbial polysaccharide gellan gum, due to the special hydrodynamic properties of gellan gum, many engineering pr...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/01
Inventor 许平王霞马翠卿
Owner SHANDONG UNIV
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