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Method for realizing directional proliferation and differentiation of stem cell

A technology for proliferation, differentiation, and stem cells, which is applied in biochemical equipment and methods, embryonic cells, germ cells, etc., to achieve the effects of mild preparation process conditions, good biocompatibility, and maintenance of biological activity

Inactive Publication Date: 2007-05-16
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

However, most of the reports on in vivo microenvironment and stem cell differentiation focus on adult stem cells, while studies on related ES cells have not been reported. The analysis may be mainly due to the fact that the in vivo implantation of adult stem cells does not need to be restricted by immune rejection.

Method used

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  • Method for realizing directional proliferation and differentiation of stem cell
  • Method for realizing directional proliferation and differentiation of stem cell

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Embodiment Construction

[0021] Preparation of APA Microcapsules: ES-D 3 Cells are maintained in vitro in growth medium. After 0.25% trypsinization and collection, adjust the cell density to 4 × 10 6 cells / ml. Using the electrostatic droplet generation method, APA microencapsulated mouse ES cells were prepared under physiological conditions. The specific preparation conditions are: 5 # Domestic syringe needle, voltage 70V, frequency 120Hz, pump speed 8.9ml / h, pH6.0-7.4, 20-37℃, 1 atmosphere, no vibration and shear. The operation process is: after mixing ES cells with 1.5% (W / V) sodium alginate solution, the cell suspension is dripped into 100mmol / L Cacl through a syringe pump 2 The solution was calcified for 20 min to obtain calcium alginate gel beads. The sodium alginate gel beads were reacted with 0.05% (W / V) polylysine (PLL) to form a film for 12 min to form a non-liquefied sodium alginate / polylysine microcapsule film, and then mixed with 0.15% (W / V) polylysine (PLL) for 12 min. / V) The sodium...

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Abstract

The invention discloses an oriental breeding and differentiating method of stem cell in the stem cell tissue engineering domain, which comprises the following steps: adopting sodium alginate and polylysine as raw material; preparing APA mouse microencapsulation embryo stem cell; transplanting mouse microencapsulation embryo stem cell into different anatomical positions of living body orientally; realizing oriental breeding and differentiation of stem cell. The invention simplifies the operation, which is fit for oriental transplantation, recycling and internal oriental inducing differentiation study in the stem cell body.

Description

technical field [0001] The invention relates to the field of stem cell tissue engineering, in particular to a new technology for realizing directional proliferation and differentiation of stem cells by utilizing tissue microenvironment in vivo. Background technique [0002] Embryonic stem cells (ESCs) are a specific group of cells derived from the blastocyst stage, with the potential of self-renewal and omnidirectional differentiation. In 1981, the successful isolation and establishment of mouse ES cells opened the prelude to stem cell research. .]. Subsequently, the directional induction and differentiation of ES cells has become a hot spot of common concern at home and abroad. At present, the research on induced differentiation of ES cells is increasingly in-depth: horizontally extending to the induced differentiation of neurons, cardiomyocytes, endothelial cells, hematopoietic cells, hepatic precursor cells, and insulin-secreting cells, and vertical to gene regulation, ...

Claims

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Application Information

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IPC IPC(8): C12N5/06C12N5/0735
Inventor 马小军王为王秀丽
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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