Process of extracting rhanolipid as biosurfactant
A rhamnolipid, biological surface technology, applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of complex composition, complex rhamnolipid process, limitations, etc., and achieve a simple operation process. Effect
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[0040] 1. Preparation of rhamnolipid fermentation broth:
[0041] 1) Inoculate Pseudomonas aeruginosa from the slant medium to the seed medium, perform shake flask culture, and fill 80 mL of seed culture in a 500 mL Erlenmeyer flask. Culture conditions: temperature 37°C, time 24 hours, rotation speed 200 rpm.
[0042] 2) Fill each 1L fermentor with 250mL of fermentation medium with soybean oil as carbon source, inoculate 7mL of Pseudomonas aeruginosa seed culture solution, culture conditions: 37℃, control the pH to 7.0 with HCl or NaOH, and The culture was shaken at 250 rpm, and the culture time was 96 hours. Approximately 230 mL of fermentation broth of each fermenter was obtained, and the fermentation broth in all the fermenters was collected together.
[0043] Note: See Table 1 and Table 2 for the formulas of the subculture slant medium and seed medium. The fermentation medium formula is: crude soybean oil 120g / L, NaNO 3 8.0g / L, KH 2 PO 4 1.0g / L, Na 2 HPO 4 .12H 2 O 1.0g / L, FeS...
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