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Process of extracting rhanolipid as biosurfactant

A rhamnolipid, biological surface technology, applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of complex composition, complex rhamnolipid process, limitations, etc., and achieve a simple operation process. Effect

Inactive Publication Date: 2007-06-06
HUNAN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

Yet, because rhamnolipid exists in the fermented liquid of microorganism, and content is very low, the maximum output of the disclosed rhamnolipid as CN1275429A is only 25g / L; The content of rhamnolipid is 30g / L~40g / L, but the components in the fermentation broth are complicated, and the process of separating and extracting relatively pure rhamnolipids is relatively complicated, and about 70-90% of its production cost will be used in the middle and lower reaches In terms of extraction and separation techniques, therefore, the large-scale application of rhamnolipids is limited

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  • Process of extracting rhanolipid as biosurfactant

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Embodiment Construction

[0040] 1. Preparation of rhamnolipid fermentation broth:

[0041] 1) Inoculate Pseudomonas aeruginosa from the slant culture medium into the seed medium, and carry out shake flask culture, and put 80mL of seed culture solution in a 500mL Erlenmeyer flask. Culture conditions: temperature 37°C, time 24 hours, rotation speed 200 rpm.

[0042] 2) Put 250mL of fermentation medium with soybean oil as carbon source in each 1L fermenter, inoculate 7mL of Pseudomonas aeruginosa seed culture solution, culture conditions: 37°C, use HCl or NaOH to control the pH value to 7.0, and The culture was shaken at 250 rpm for 96 hours. Obtain about 230 mL of fermentation broth in each fermentor, and collect the fermentation broth in all fermentors together.

[0043] Note: See Table 1 and Table 2 for the formulations of subculture slant medium and seed medium. The formulation of the fermentation medium is: crude soybean oil 120g / L, NaNO 3 8.0g / L,KH 2 PO 4 1.0g / L, Na 2 HPO 4 .12H 2 O 1.0g / ...

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Abstract

The present invention is process of extracting rhamnolipid as biosurfactant. The process includes the following steps: centrifuging rhamnolipid fermenting liquid at 4 deg.c in 8000 rpm to eliminate thallus, adding ammonium sulfate in 60-120 mg / L, stilling in a fridge at 4 deg.c for 10 hr, and centrifuging to eliminate protein precipitate; diluting with icy salt solution, eliminating protein precipitate to obtain supernatant, stilling in a fridge at 4 deg.c for residual grease to demulsify and agglomerate in the surface of the supernatant, skimming the grease; regulating the solution pH to below 2, stilling in a fridge at 4 deg.c for 10 hr; centrifuging at 4 deg.c and 8000 rpm for 30 min and freeze drying the collected yellowish precipitate to obtain rhamnolipid product. The technological process of the present invention features low production cost and environment friendship.

Description

technical field [0001] The present invention relates to a method for the extraction of biosurfactants produced by microorganisms. Background technique [0002] Rhamnolipid is a surface-active amphiphilic compound produced by microorganisms. Compared with chemically synthesized surfactants, it has the same characteristics as reducing surface tension, stabilizing emulsions, and foaming. Its surface activity and emulsification The ability is stronger, and it also has the characteristics of non-toxicity and easy biodegradation that general chemically synthesized surfactants do not have. It is a green product that is conducive to environmental protection. Rhamnolipids have various types of molecular structures, have many special functional groups, have strong specificity, and can be used in some special fields. It has great potential application value in industries such as oil recovery, environmental remediation, medicine, cosmetics, detergent and food. [0003] However, the yi...

Claims

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Application Information

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IPC IPC(8): C07H15/04C07H1/06B01F17/56E21B43/22C12P19/02C09K23/56
Inventor 施周马满英吴星杰
Owner HUNAN UNIV
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