Method for separating and purifying lentinan

A technology for separation and purification of lentinan, which is applied in the field of separation and purification of lentinan, can solve the problems of low yield of separation and purification process, large loss of polysaccharide, poor process reproducibility and operability, and achieve high yield and low cost Effect

Inactive Publication Date: 2007-06-13
成都普瑞法科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The separation and purification process has low yield, large polysaccharide loss, and poor process reproducibility and operability

Method used

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  • Method for separating and purifying lentinan

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Take 25kg of clean fresh shiitake mushrooms, mash them, add water and decoct twice, the first time for 6 hours, the second time for 3 hours, filter, and combine the filtrates. Concentrate the filtrate, add ethanol to precipitate, let stand, filter, wash with ethanol, dissolve the precipitate with sodium carbonate solution, filter, adjust the pH of the filtrate to 7.0, concentrate under reduced pressure, add ethanol to precipitate, stand still, filter, and precipitate with acetone Wash and dry under reduced pressure to obtain crude lentinan.

[0029] The crude lentinan was dissolved in sodium carbonate solution, filtered, and the filtrate was pretreated and neutralized by DEAE-cellulose pre-column. Ultrafiltration, concentrate the concentrate under reduced pressure, add to DEAE-cellulose to allow it to fully absorb. Then put it on the column, wash with water, and finally elute with sodium hydroxide solution, neutralize, ultrafilter with hollow fiber membrane, concentrat...

Embodiment 2

[0031] Take 3kg of clean dried shiitake mushrooms, mash them, extract three times with 0.25mol / l sodium carbonate solution, combine the extracts, neutralize, concentrate, add ethanol to make the alcohol content reach 70-75%, let it stand, and filter , wash with ethanol, dissolve the precipitate with sodium carbonate solution, filter, adjust the pH of the filtrate to 7.0, concentrate under reduced pressure, add ethanol to precipitate, let stand, filter, wash the precipitate with acetone, and dry under reduced pressure to obtain crude lentinan.

[0032] The crude lentinan was dissolved in sodium bicarbonate solution, filtered, and the filtrate was pretreated by DEAE-cellulose pre-column, neutralized, and ultrafiltered. Concentrate the concentrated solution under reduced pressure and add it to DEAE-cellulose to allow it to fully absorb. Then put on the column, wash with water, and finally elute with 0.5mol / L sodium hydroxide solution, collect the positive part of the polysacchari...

Embodiment 3

[0034] Take 25kg of clean fresh shiitake mushrooms, mash them, add water and decoct twice, the first time for 6 hours, the second time for 3 hours, filter, and combine the filtrates. The residue was leached twice with 0.25mol / l sodium carbonate solution, combined the extracts, after neutralization, combined with the water extract, concentrated, added ethanol for precipitation, left standing, filtered, washed with ethanol, and sodium carbonate solution was used for precipitation solution, filtered, the filtrate was adjusted to pH 7.0, concentrated under reduced pressure, added ethanol to precipitate, allowed to stand, filtered, the precipitate was washed with acetone, and dried under reduced pressure to obtain crude lentinan.

[0035] The crude lentinan was dissolved in sodium bicarbonate solution, filtered, and the filtrate was pretreated and neutralized by DEAE-cellulose pre-column. Concentrate the concentrated solution under reduced pressure and add it to DEAE-cellulose to a...

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Abstract

The invention provides a Lentinan Purification method. It is lentinan extract, concentrate, alcohol, after alkali - soluble then alcohol, alkali - soluble of precipitation then through pretreatment, desalination, DEAE-column chromatography, ultrafiltration, not through part condensed, Freeze-drying or by acetone precipitation, to obtain Lentinan. This method can produce large, high yield, good quality products.

Description

technical field [0001] The invention provides a separation and purification method of lentinan. Background technique [0002] Lentinan is an active substance isolated from shiitake mushrooms with obvious anti-cancer effects. It was first discovered in Japan in 1969. It was first produced in Japan in 1986 and prepared into powder injections for sale abroad. It is mainly used clinically for gastric cancer, For rectal cancer and other digestive system cancers, its main route of administration is intravenous drip or injection. In 1988, my country began to import Japanese Ajinomoto lentinan and apply it clinically. Since 1982, Sichuan Institute of Antibiotics and Henan Institute of Pharmaceutical Industry have successively extracted lentinan from the liquid of shiitake mushrooms cultured by submerged fermentation, named "lentinan" to distinguish the lentinan extracted from fruiting bodies. In 1986, Henan Kaifeng Pharmaceutical Factory produced Lentinan tablets. In the same year,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 吴四灵王小林
Owner 成都普瑞法科技开发有限公司
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