Process for nano colloid gold for immune detection and reaction apparatus therefor

A reaction device and nano-colloid technology, which is applied in the field of preparation of nano-colloidal gold for immunoassay and its reaction device, can solve the problems of water volatilization, uneven stirring, affecting the size difference of colloidal gold particles, sphericity particle size distribution, etc. , to achieve the effect of good sphericity, narrow particle size distribution and small particle size difference

Inactive Publication Date: 2007-06-13
GENERAL RESEARCH INSTITUTE FOR NONFERROUS METALS BEIJNG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

During the reaction process, the traditional process has defects such as uneven heating of the reaction system, severe water volatilization during the heating process, and uneven stirring, which greatly affect the size difference, sphericity, and particle size distribution of the obtained colloidal gold particles. prone to "dry spots"

Method used

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  • Process for nano colloid gold for immune detection and reaction apparatus therefor
  • Process for nano colloid gold for immune detection and reaction apparatus therefor
  • Process for nano colloid gold for immune detection and reaction apparatus therefor

Examples

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Embodiment 1

[0027] Embodiment 1, adopt the reaction apparatus shown in the present invention to prepare, and reaction conditions are: reaction temperature is 60 ℃, stirring rate is 300rpm, mass percentage content 1% trisodium citrate add-on 4mL, 0.025mol / L K 2 C0 3 The addition amount is 0.6mL, and the addition amount of 1% tannic acid is 0.6mL, and the mass percentage composition 1% HAuCl 4 The amount added was 1 mL, and the reaction time was 10 min.

[0028] Test results: The TEM photo of colloidal gold is shown in Figure 2. Colloidal gold particles have very good sphericity, narrow particle size distribution, and small particle size differences. There is no floating matter (no dry point) on the surface of the colloidal gold solution, and no coagulation phenomenon is found; the average particle size of the colloidal gold is 5.07nm, and Fig. 4 is a particle size distribution diagram; the colloidal gold is placed in a sealed place at 4°C for 30 days and no coagulation phenomenon occurs ...

Embodiment 2

[0029] Embodiment 2, prepare with reaction apparatus shown in the present invention, reaction conditions are: reaction temperature is 100 ℃, reaction time is 10min, stirring rate is 300rpm, mass percent content 1% trisodium citrate add-on 12.2mL, mass Percent content 1% HAuCl 4 The amount added is 3 mL.

[0030] Test results: The TEM photo of colloidal gold is shown in Figure 3. Colloidal gold particles have very good sphericity, narrow particle size distribution, and small particle size differences. There is no floating matter (no dry point) on the surface of the colloidal gold solution, and no coagulation phenomenon is found. The average particle size of the colloidal gold was measured to be 14.88nm, and Figure 5 is a particle size distribution diagram; the colloidal gold was sealed at 4°C for 30 days without coagulation.

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Abstract

The invention relates to a technology method and reacting device for making detection using nanometer colloidal gold that includes the following steps: setting material intake on reacting device, and having sealing cover, whisking device is set in the reacting device, and condensate reflux unit is set on top, heater is set on the bottom of the reactor and the four sides of bottom to form heating area to take equal heating to reaction liquid; adding auric chloride acid solution into reacting device to take heating, starting whisking device and taking cooling by using condensate reflux unit; after solution boiling, adding citric acid-3-natrium solution, after boiling for 10 minutes, stop heating; cooling and filtering to gain colloidal gold. The invention could gain the colloidal gold that has good sphericity and size distribution.

Description

technical field [0001] The invention relates to a new process for synthesizing nano colloidal gold for immunity and a reaction device thereof. Background technique [0002] The currently widely used technique for preparing nano colloidal gold for immunoassay is trisodium citrate reducing chloroauric acid solution method. During the reaction process, the traditional process has defects such as uneven heating of the reaction system, severe water volatilization during the heating process, and uneven stirring, which greatly affect the size difference, sphericity, and particle size distribution of the obtained colloidal gold particles. Prone to "dry spots". Contents of the invention [0003] The purpose of this invention is to provide a kind of processing method for preparing nano-colloidal gold for immunoassay, and the colloidal gold obtained compares with the colloidal gold prepared by the current method in particle size difference, sphericity, particle size distribution and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/52
Inventor 熊晓东贺昕梁敬博陈峤王胜国张曦
Owner GENERAL RESEARCH INSTITUTE FOR NONFERROUS METALS BEIJNG
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