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Delivery of disease control in aquaculture and agriculture using microbes containing bioactive proteins

a technology of bioactive proteins and delivery systems, applied in the field of animal feeds, can solve the problems of crude fermentation broths or crude preparations including cells, crude preparations of crude microorganisms, and no use of recombinant microorganisms in agriculture or agriculture, and achieve the effects of reducing the number of microorganisms, and reducing the number of antibiotics

Inactive Publication Date: 2004-03-11
ADVANCED BIONUTRITION CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such recombinant microbes have not been used in agriculture or agriculture, wherein the cultivated animal ingests the whole organism.
However, crude fermentation broths, or crude preparations including cells, have not been used for any kind of therapeutic delivery system.
However, only a very few of these microbes, perhaps less than 10 species, have been exploited for aquaculture feeds.
The marine environment is filled with bacteria and viruses that can attack fish and shellfish, thereby devastating aquaculture farms very quickly.

Method used

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  • Delivery of disease control in aquaculture and agriculture using microbes containing bioactive proteins
  • Delivery of disease control in aquaculture and agriculture using microbes containing bioactive proteins

Examples

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Example 1

[0054] Selection of Useful Microbial Sources for Feeds that Provide Disease Control. Microalgal biomass samples, aqueous extracts, organic extracts, and extracts from the growth medium after cultivation of the algae were concentrated and spotted on filter paper discs. Using sterile techniques, these discs were then placed on agar plates overlaid with a lawn of selected test organisms including, but not limited to, gram-negative bacteria, gram-positive bacteria, antibiotic resistant bacteria, yeast, or other fungi. After incubation for an appropriate length of time to allow growth of the lawn of test organism, the plates were observed for zones of clearing (non-growth) around the filter paper discs. Large zones of clearing indicate potent antibiotic activity; small zones of clearing indicate less potent antibiotic activity.

example 2

[0055] Incorporation of an Antibody into an Algal Feed. A particular viral or bacterial pathogen is chosen and used to prepare monoclonal antibodies using procedures well known to those in this field (Harlow and Lane, eds., 1988. Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press). Gene(s) coding for this antibody or an appropriate antibody fragment (F.sub.ab or F.sub.v) are isolated and amplified in the appropriate vector. The gene is spliced into a transformation vector suitable for a eukaryotic alga (e.g. Chlorella) or a prokaryotic alga (e.g. Synechocystis), or a yeast (e.g. Saccharomyces) or a fungus (e.g. Mortierella). The transformation vector is chosen so that the antibody will be over-expressed in the microbial cell biomass. This biomass is then used as a feed additive in such a way as to provide the antibody directly to the animal, thus providing passive immunity.

example 3

[0056] Expression of a Bactericidal Protein in a Microbial Feed. A bactericidal protein is chosen for the particular application. For example, proteins of the penaeidin class may be chosen for pathogenic control in shrimp. Penaeidins are members of a family of antimicrobial peptides isolated from crustaceans (e.g., Penaeid shrimp). Antimicrobial peptides can also come from insects and chelicerates, and can include, but are not limited to, cecropins, peneaidins, bactenecins, callinectins, myticins, tachyplesins, clavanins, misgunins, pleurocidins, parasins, histones, acidic proteins, and lysozymes. The gene for the chosen protein or peptide is either isolated from the original source, an amplification source, or made synthetically. The gene is then incorporated into a transformation vector suitable for a eukaryotic alga (e.g. Chlorella) or a prokaryotic alga (e.g. Synechocystis), or a yeast (e.g. Saccharomyces) or a fungus (e.g. Mortierella). The transformation vector is chosen so th...

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Abstract

A microbial biomass, made from algae, bacteria, fungi, yeast, or combinations thereof, provides a feed for animals raised either in agriculture or aquaculture. A feed additive, and a therapeutic composition can also be made from a microbial biomass of algae, bacteria, fungi, yeast, or combinations thereof. The feed, feed additive, and therapeutic composition can comprise one or more proteins, peptides, antibodies, antibody fragments, or a combination thereof, wherein said proteins, peptides, antibodies, antibody fragments, or a combination thereof are non-native to the microbes of the biomass. The biomass can have therapeutic, bioactive, nutritional, and / or immunogenic properties.

Description

[0001] This application is a Continuation-In-Part application of PCT / US02 / 08651, filed Mar. 22, 2002, which claims the benefit of U.S. Provisional Application No. 60 / 277,947, filed Mar. 23, 2001, the entire disclosures of which are incorporated by reference.[0002] 1. Field of the Invention[0003] This invention is directed to animal feeds used in aquaculture or in agriculture, with microbial cells as components. These microbial cells contain exogenous peptides, proteins, and / or antibodies, which can convey resistance or immunity to pathogens (such as viral or bacterial), or otherwise improve the health and performance of the species that consume them. The microbial cells can be algae, bacteria, fungi, or yeast. The exogenous peptides, proteins, and / or antibodies can be expressed inside the microbial cells by direct genetic modification of the microbe or by infecting the microbe with a virus that has been altered to express the protein of interest. The invention is also directed to an...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/16A23K1/18A23K20/195A23L17/60A61K35/66A61K35/74A61K35/742A61K35/745A61K35/747A61K36/02A61K36/06A61K38/00A61K39/395A61K45/00A61P31/04A61P31/12C12N1/00C12N15/31C12P21/08
CPCA23K1/006A23K1/009A23K1/1631A23K1/188A61K36/02A61K36/06A61K35/742A61K35/745A61K35/747A23L1/337A23K10/10A23K10/18A23K20/147A23K50/80A23L17/60A61P31/04A61P31/12
Inventor KYLE, DAVID J.
Owner ADVANCED BIONUTRITION CORP
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