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Substrates and compounds bonded thereto

a technology applied in the field of substrates and compounds bonded thereto, can solve the problems of incomplete or inefficient immobilization of nucleophile-containing materials on the substra

Inactive Publication Date: 2005-06-30
3M INNOVATIVE PROPERTIES CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0007] The present invention provides articles and methods for the use of such articles in immobilizing nucleophile-containing materials such as amine-containing analyte, amino acid, peptide, DNA, RNA, protein, enzyme, organelle, immunoglobulin, and fragments and combinations of two or more of the foregoin

Problems solved by technology

Hydrolysis can result in incomplete or inefficient immobilization of the nucleophile-containing materials on a substrate.

Method used

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  • Substrates and compounds bonded thereto
  • Substrates and compounds bonded thereto
  • Substrates and compounds bonded thereto

Examples

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example 1

[0149] A functionalized porous membrane coated with diamond-like glass (DLG) was prepared. A 5 cm2 high density polyethylene thermally induced phase separation (HDPE TIPS) membrane (obtained from 3M Company, St. Paul, Minn.) with a pore size of about 0.09 um and having a thickness of about 23 micrometers was diamond like glass (DLG) coated, using a plasma process as described in EP 1 266 045 B1 (David et al) to extend the DLG coating into the pores of the TIPS membrane. The DLG-coated TIPS membrane was placed in 50 ml of ethanol containing 2% by volume 3-amino propyl triethoxy silane (Sigma-Aldrich, St. Louis, Mo.), 1 ml water and few drops of 0.1N acetic acid. After 10 minutes in this solution the membrane was removed and washed with ethanol and dried.

[0150] A PNC trimer was tethered on the functionalized membrane by placing the membrane in 20 ml of a toluene solution containing 0.2 g of PNC (Sigma Aldrich, St. Louis, Mo.) which was purified by sublimation. The amino group of the ...

example 2

[0154] Glass slides were treated with DLG using the following conditions. Each glass slide was etched in oxygen plasma for 10 seconds and exposed to a mixture of tetramethylsilane and oxygen plasma for 20 seconds followed by oxygen plasma for another 10 seconds. The DLG coated glass slides were then placed in a 1% solution of 3-aminopropyltriethoxy silane in ethanol for 10 minutes. Thereafter, the glass slides were removed and washed with ethanol and dried under a nitrogen flow. The dried glass slides were reacted with phosphoric chloride in toluene (Sigma Aldrich, St. Louis, Mo.). The reaction time was varied from several minutes up to one hour. Contact angle measurements were taken to monitor and confirm attachment of the PNC to the aminopropyltriethoxy silane attached to the DLG substrate. The amine has a low contact angle of 20 degrees, which on reaction with PNC increases to 45 degrees and which stabilized in about 10 minutes. Contact angle data for the attachment of the PNC is...

example 3

[0156] An approximately 20 cm by 30 cm polyimide film (obtained from E.I. du Pont de Nemours & Co., Wilmington, Del. under the trade designation “KAPTON E”) was first coated with diamond-like carbon (DLC) followed by diamond-like glass (DLG). The polyimide film was affixed to the powered electrode of a Model 2480 parallel-plate capacitively coupled reactive ion etcher (Plasma Therm, St. Petersburg, Fla.) using 3M 811 adhesive tape (3M Company, St. Paul, Minn.). DLC was deposited onto the polyimide membrane using an acetylene plasma. The ion etcher chamber was closed and the chamber was pumped to a pressure of 0.67 Pa (0.005 Torr). Oxygen gas was introduced into the chamber at a flow rate of 500 standard cm3 per minute, and the pressure of the chamber was maintained at 6.7 Pa (0.050 Torr). Plasma was ignited and was sustained at a power of 2000 W for 15 seconds. The oxygen gas flow was then terminated and the chamber was allowed to pump to a pressure of 0.67 Pa (0.005 Torr). Acetylen...

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Abstract

Articles and methods for the use of such articles are described for use in immobilizing nucleophile-containing materials. In one aspect, the invention provides an article comprising: a substrate having a first surface and a second surface; and a phosphonitrilic tethering group affixed to the first surface of the substrate, the phosphonitrilic tethering group comprising a reaction product of a complementary functional group on the first surface of the substrate with a phosphonitrilic tethering compound. A method of immobilizing a nucleophile-containing material to a substrate is also described, the method comprising: providing a phosphonitrilic tethering compound; providing a substrate having a complementary functional group capable of reacting with phosphonitrilic tethering compound; preparing a substrate-attached phosphonitrilic tethering group by reacting the phosphonitrilic tethering compound with the complementary functional group on the substrate resulting in an ionic bond, covalent bond, or combinations thereof; and reacting the substrate-attached phosphonitrilic tethering group with a nucleophile-containing material to immobilize the nucleophile-containing material.

Description

[0001] This application claims the benefit of U.S. Provisional Application No. 60 / 533,178, filed Dec. 30, 2003.FIELD OF THE INVENTION [0002] The invention relates to articles comprising a substrate having a tethering group affixed to the substrate and to methods for immobilizing a nucleophile-containing material to the substrate. BACKGROUND OF THE INVENTION [0003] The covalent attachment of biologically active molecules to the surface of a substrate can be useful in a variety of applications such as in diagnostic devices, affinity separations, high throughput DNA sequencing applications, the clean-up of polymerase chain reactions (PCR), and the like. Immobilized biological amines, for example, can be used for the medical diagnosis of a disease or genetic defect or for detection of various biomolecules. [0004] The modification of solid supports (e.g. particulate chromatography supports) by introduction of reactive functional groups for the immobilization of any of a variety of ligand...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/545G01N33/552
CPCG01N33/54353Y10T428/28Y10T428/2804Y10T428/2852Y10T428/249953
Inventor LAKSHMI, BRINDA B.
Owner 3M INNOVATIVE PROPERTIES CO
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