Recombinant MVA virus, and the use thereof

Inactive Publication Date: 2007-03-29
GSF FORSCHUNGSZENT FUR UMWELT & GESUNDHEIT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, important future applications of the vaccinia virus/T7 RNA polymerase hybrid system, as e.g. to generate recombinant proteins or recombinant viral particles for novel therapeutic or prophylactic approaches in humans, might be hindered by the productive replication of the recombinant vaccinia vector.
Therefore the most

Method used

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  • Recombinant MVA virus, and the use thereof
  • Recombinant MVA virus, and the use thereof
  • Recombinant MVA virus, and the use thereof

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1. Growing and Purification of the Viruses

1.1 Growing of the MVA Virus

[0086] The MVA virus is a highly attenuated vaccinia virus derived from the vaccinia virus strain Ankara (CVA) by long-term serial passages on primary chicken embryo fibroblast (CEF) cultures. For a general rewiew of the history of the production, the properties and the use of MVA strain, reference may be made to the summary published by Mayr et al., in Infection, 3:6-14 (1975). Due to the attenuation in CEF, the MVA virus replicates to high titers in this avain host cell. In mammalian cells, however, MVA is severely growth restricted, and typical plaque formation by the virus is not detectable. Therefore, MVA virus was grown on CEF cells. To prepare CEF cells, 11-day-old embryos were isolated from incubated chicken eggs, the extremities are removed, and the embryos are minced and dissociated in a solution composed of 0.25% trypsin at 37° C. for 20 minutes. The resulting cell suspension was filtered and cells ...

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Abstract

The present invention relates to recombinant vaccinia viruses derived from the modified vaccinia virus Ankara (MVA) and containing and capable of expressing foreign genes which are inserted at the site of a naturally occurring deletion in the MVA genome, and the use of such recombinant MVA viruses for the production of polypeptides, e.g. antigens or therapeutic agents, or viral vectors for gene therapy, and the use of such recombinant MVA viruses encoding antigens as vaccines.

Description

RELATED APPLICATION(S) [0001] This application is a division of U.S. application Ser. No. 10 / 147,284, filed May 15, 2002, which is a continuation of U.S. application Ser. No. 09 / 002,443, filed Jan. 2, 1998, which is a continuation of International Application No. PCT / EP96 / 02926, which designated the United States and was filed on Jul. 3, 1996, published in English, which claims the benefit of Danish Patent Application No. DK 0782 / 95, filed Jul. 4, 1995. The entire teachings of the above application(s) are incorporated herein by referenceBACKGROUND OF THE INVENTION [0002] Vaccinia virus, a member of the genus Orthopoxvirus in the family of Poxviridae, was used as live vaccine to immunize against the human smallpox disease. Successful world-wide vaccination with vaccinia virus culminated in the eradication of variola virus, the causative agent of the smallpox (The global eradication of smallpox. Final report of the global commission for the certification of smallpox eradication. Histo...

Claims

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Application Information

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IPC IPC(8): A61K39/12C07H21/04C12N15/86A61K31/00C12N15/09A61K35/76A61K39/00A61K39/015A61K39/04A61K39/145A61K39/21A61K39/245A61K39/29A61K48/00A61P31/00A61P31/12A61P31/16A61P31/18A61P31/22C07K14/16C12N1/21C12N5/00C12N5/10C12N7/00C12N7/01C12N9/02C12N9/12C12N15/49C12N15/53C12N15/54C12N15/63C12N15/863
CPCA61K48/0091A61K2039/5256C07K14/005C12N7/00C12N2740/16322C12N9/1247C12N15/86C12N2710/24143C12N9/0059A61P31/00A61P31/12A61P31/16A61P31/18A61P31/22A61P35/00A61P37/04Y02A50/30C12N15/52
Inventor SUTTER, GERDOHLMANN, MARIONERFLE, VOLKER
Owner GSF FORSCHUNGSZENT FUR UMWELT & GESUNDHEIT
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