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Method for stimulating intestinal barrier integrity after non-natural birth

a technology of intestinal barrier and non-natural birth, which is applied in the direction of drug composition, plant/algae/fungi/lichens, immunological disorders, etc., can solve the problems of high content of (undesirable, risk of pathogenic infection), and achieve the effects of preventing infection, reducing the occurrence, and accelerating the colonization of pathogenic bacteria

Inactive Publication Date: 2009-10-22
NV NUTRICIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention relates to a nutritional composition that aims to improve the intestinal flora and maturation of infants delivered via caesarean section. The composition contains long-chain polyunsaturated fatty acids (LC-PUFA) and nucleotides which are known to promote the adhesion of lactic acid producing bacteria to mucosal surfaces, decrease the incidence of infections, prevent food allergies and atopic diseases, and dampen intestinal inflammation. The composition can be easily incorporated into a nutritional product for infants without adding too much burden on them.

Problems solved by technology

Additionally it was found that the intestinal flora of infants born via caesarean section has a high content of (undesirable) Escherichia coli 6 weeks after delivery.
These infants are normally delivered in a hospital environment, which is a risk for pathogenic infection due to the occurrence of nosocomial bacteria.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Molecular Characterization of Intestinal Microbiota in Infants Born by Vaginal Delivery Vs. Caesarean Delivery

[0050]In the present study the influence of mode of delivery (caesarean delivery versus vaginal delivery) on the intestinal microbial composition at the third day of life was studied using by PCR amplification with species-specific primers for ten Bifidobacterium species, three Ruminococcus species and one Bacteroides species.

[0051]The microbial DNA was extracted and analysed according to Favier et al, Environ Microbiol 2002; 68:219-226 and Satokari et al, Appl Environ Microbiol 2001; 67:504-513; Satorkari et al System Appl Microbiol 2003; 26:572-584.

[0052]The results of the Bifidobacterium and other species detected in faecal samples of 21 newborns after caesarean delivery obtained at the 3rd day of life are given in Table 1. Table 2 gives the Bifidobacterium and other species detected in faecal samples of 21 newborns after vaginal delivery obtained at the 3rd day of life. ...

example 2

Effect of LC-PUFA on Barrier Integrity

[0055]Monolayers (MC) of intestinal epithelial cell lines T84 were incubated in the luminal compartment with different 100 μM polyunsaturated fatty acids ARA (arachidonic acid; 5,8,11,14-eicosatetraenoic acid), DHA (cis-4,7,10,13,16,19 docosahexaenoic acid), EPA (eicosapentaenoic acid) or control palmitic (C 16:0) acid (Palm) (Sigma, St. Louis, USA) for 0, 24, 48 and 72 hr. The epithelial barrier function was determined by measuring the transepithelial resistance (TER, Ω.cm2) by epithelial volt-ohm meter (EVOM; World Precision Instruments, Germany) and permeability for 4 kD FITC dextran (paracellular permeability marker, Sigma, USA).

[0056]Results of the effect of fatty acids (100 μM) on spontaneous barrier integrity after 72 hr incubation are given in Table 3. Table 3 shows that the LC-PUFA's ARA, EPA and DHA reduce the molecular flux and improve epithelial resistance. In contrast the control palmitic acid has the opposite effects, i.e. compromi...

example 3

Effect of LC-PUFA on IL-4 Mediated Barrier Disruption

[0057]Monolayers (MC) of intestinal epithelial cell lines T84 were incubated in the presence of IL-4 (2 ng / ml, serosal compartment, Sigma, USA) with or without polyunsaturated fatty acids ARA, DHA, GLA, EPA, or control palmitic acid (10 μM or 100 μM, mucosal compartment, Sigma, St. Louis, USA). Cells were pre-incubated with ARA, DHA, EPA, or palmitic acid for 48 hr prior to the IL-4 incubation. The co-incubation of PUFA's and palmitic acid with IL-4 was continued for another 48 hr, while culture medium and additives were changed every 24 hr. The epithelial barrier function was determined by measuring the transepithelial resistance (TER) and permeability as described in example 2.

[0058]Results of the effect of ARA, DHA, EPA and palmitic acid (100 μM) on IL-4 mediated barrier disruption are given in Table 4. Table 4 shows that the LC-PUFA's ARA, DHA and EPA inhibit the increased flux caused by IL-4. In contrast palmitic acid had a d...

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Abstract

The present invention relates to methods for feeding and stimulating the health of infants delivered via caesarean section comprising administering long chain polyunsaturated fatty acids and / or nucleotides

Description

FIELD OF THE INVENTION[0001]The present invention relates to methods for feeding and therapy infants delivered via caesarean section.BACKGROUND OF THE INVENTION[0002]Infants have an immature intestine at birth. After birth, the neonatal gastro-intestinal tract undergoes rapid growth and maturation, including mucus formation and maturation and closing of tight junctions to allergens, toxins and pathogenic micro-organisms. In human milk several factors are present which improve gut maturation.[0003]A permeable, immature intestinal barrier plays a role in the acquirement of intestinal inflammation, infections, diarrhea, and atopic diseases including food allergy. A properly maturing intestine, especially in combination with a healthy intestinal flora, results in a reduced incidence of infections, a strengthened immune system, a reduced incidence of food allergy and / or other atopic diseases, and / or a reduced incidence of diarrhea, constipation and / or intestinal inflammation.[0004]Intest...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A23K1/18A23C11/02A23C11/10A23K1/16A23L11/10A23L29/00A23L29/231A23L29/256A23L33/00A61K35/745A61K35/747
CPCA23C9/1234A61K47/36A23C9/206A23L1/0345A23L1/296A23L1/3014A23L1/308A23V2002/00A23Y2220/85A23Y2300/65A61K31/202A61K31/702A61K31/7068A61K31/7072A61K31/7076A61K31/708A61K31/732A61K31/734A61K35/745A61K35/747A23C9/203A61K35/74A23V2200/32A23V2200/3204A23V2200/3202A23V2200/30A23V2200/324A23V2250/28A61K2300/00A23L33/21A23L29/065A23L33/10A23L33/12A23L33/135A23L33/17A23L33/40A61K31/7016A61K31/7064A23V2400/187A23V2400/513A23V2400/521A23V2400/527A23V2400/529A23V2400/515A23V2400/517A23V2400/519A23V2400/535A23V2400/537A23V2400/531A23V2400/533A23V2400/151A23V2400/125A23V2400/157A23V2400/113A23V2400/143A23V2400/165A23V2400/173A23V2400/175A23V2400/249A23V2400/169A23V2250/284A23V2250/5042A23V2250/5062A23V2250/5114A61K31/716A61K31/733A61K35/742A61K35/744A61K36/064A61P1/00A61P1/12A61P1/14A61P11/02A61P11/06A61P17/00A61P27/02A61P31/04A61P37/02A61P37/08Y02A50/30
Inventor SCHMITT, JOACHIMBOEHM, GUNTHER
Owner NV NUTRICIA
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