Composition for preventing or treating liver toxicity comprising galgeuntang or lactic acid bacteria fermented galgeuntang
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example 1
Preparation of Lactic Acid Bacteria Fermented Galgeuntang
[0038]Bifidobacterium breve (KFRI 744, ATCC 15700) was obtained from KFRI (Korea Food Research Institute) and used for the following experiments.
Seed Culture of Lactic Acid Bacteria
[0039]The distributed strain was sub-cultured in slant medium and liquid medium. First, the lactic acid bacteria were inoculated in slant medium, followed by culture in a 37° C. incubator for 24 hours. When colonies were formed, oxygen supply was blocked by using paraffin film, which was stored in a refrigerator. To avoid losing the activity of the bacteria or contamination by other microorganisms, the medium was replaced with fresh slant agar medium every 2˜3 weeks. The colonies were inoculated in liquid medium, followed by culture in a 37° C. incubator for 24 hours.
[0040]RCM (Reinforced Clostridial Medium: 10 g / L Bacto-tryptose, 10 g / L Bacto-beef extract, 3 g / L Bacto-yeast extract, 5 g / L Bacto-dextrose, 5 g / L Sodium chloride,...
experimental example 1
Treatment Effect on Liver Toxicity
Test Animals
[0058]Male rats at 8 weeks were obtained from Orient Co. Ltd. (459-24, Gasan-dong, Geumcheon-gu, Seoul), which were then quarantined and acclimated in an animal lab of KIOM (Korea Institute of Oriental Medicine) for 2 weeks. Then, healthy animals were selected for the experiment.
Determination of Dosage
[0059]To investigate the liver protective effect of the Galgeuntang prepared by traditional oriental medicine prescription and the lactic acid bacteria fermented Galgeuntang of the present invention, rats were treated with carbon tetrachloride (CCl4) for 24 hours, and then damage of liver was examined. Administration dosage of Galgeuntang was determined as 15 ml / kg based on daily intake (3 times, 3 packs) and dosage of the fermented Galgeuntang was determined as 7.5, 15, and 30 ml / kg.
Animal grouping and identification
[0060]Animal grouping was performed as follows. First, those rats selected as healthy during acclimation were weighed. Ani...
experimental example 2
Histopathological Examination with Liver Tissues
[0078]In this example, histopathological examination was performed to investigate the effect of the experimental samples in the test animals induced with liver toxicity by the administration of carbon tetrachloride. The test animals were 30 male rats in total. Livers of the rats were fixed in 10% neutral formalin solution and then examined. Total 6 experimental groups including the normal control group were prepared as shown in Table 8.
TABLE 8IdentificationNumber ofnumber ofGroupCCl4Test materialanimalanimalCONX*X51~5NCT◯X5 6~10GGT◯Galgeuntang511~15GFT1◯Fermented Galgeuntang516~20(low concentration)GFT2◯Fermented Galgeuntang521~25(medium concentration)GFT3◯Fermented Galgeuntang526~30(high concentration)
[0079]Each test animal treated with carbon tetrachloride was evaluated by centrilobular necrosis, vacuolar degeneration, and inflammatory cells infiltration in liver cells. To compare the alleviation levels in experimental groups, averag...
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