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Phosphoinositide 3-kinase inhibitor with a zinc binding moiety

a phosphoinositide and zinc binding technology, applied in the field of phosphoinositide 3kinase inhibitors with zinc binding moiety, can solve the problems of low in vivo anti-tumor activity, oncogenic transformation and cancer, and treatment regimes using a cocktail of cytotoxic drugs often are limited by dose-limiting toxicities, so as to achieve potent antiproliferative activity, potent inhibitory activity, and advantageous properties

Active Publication Date: 2013-04-11
CURIS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The compounds described in this patent have the ability to treat a variety of cancers and other diseases associated with PI3 kinase and HDAC activity. These compounds can target multiple molecular targets and have enhanced activity compared to separate molecules with separate PI3K and HDAC inhibitory activities. The compounds have a favorable safety profile and can overcome the limitations of inhibitors that target single genes or pathways. The compounds can disrupt cancer networks, induce cell cycle arrest and apoptosis, and overcome drug resistance. Therefore, the patent describes a new approach to treat cancer and related diseases that targets multiple pathways simultaneously.

Problems solved by technology

Although Wortmannin is a potent PI3K inhibitor with a low nanomolar IC50 value, it has low in vivo anti-tumor activity.
In the case of tumor suppressor genes, transcriptional silencing due to histone modification can lead to oncogenic transformation and cancer.
Certain cancers have been effectively treated with such a combinatorial approach; however, treatment regimes using a cocktail of cytotoxic drugs often are limited by dose limiting toxicities and drug-drug interactions.
However, the ability to use such combinations currently is limited to drugs that show compatible pharmacologic and pharmacodynamic properties.
In addition, the regulatory requirements to demonstrate safety and efficacy of combination therapies can be more costly and lengthy than corresponding single agent trials.
Once approved, combination strategies may also be associated with increased costs to patients, as well as decreased patient compliance owing to the more intricate dosing paradigms required.

Method used

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  • Phosphoinositide 3-kinase inhibitor with a zinc binding moiety
  • Phosphoinositide 3-kinase inhibitor with a zinc binding moiety
  • Phosphoinositide 3-kinase inhibitor with a zinc binding moiety

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of N-hydroxy-2-(((2-(6-methoxypyridin-3-yl)-4-morpholinothieno[3,2-d]pyrimidin-6-yl)methyl)(methyl)amino)pyrimidine-5-carboxamide (Compound 1)

Step a: (Z)-Ethyl-2-(ethoxymethyl)-3-methoxyacrylate (Compound 202)

[0138]Sodium (40.9 g, 1.78 mol) was added to ethanol (750 mL) in portions carefully and the solution was concentrated to give NaOEt powder after all sodium metal disappeared. Under stirring, hexane (1.0 L) was added and the mixture was cooled with ice-water bath. A mixture of 201 (130 g, 0.89 mol) and ethyl formate (131 g, 1.78 mol) was added dropwise at 0-5° C. The reaction mixture was stirred at room temperature overnight. Dimethyl sulfate (224 g, 1.78 mol) was added dropwise with cooling of ice-water bath. The resulting mixture was heated at 50° C. for 2 h. To the mixture was added triethylammonium chloride (122 g) and sodium hydroxide (20 g). The mixture was then stirred at room temperature for 4 h and filtered. The filtrate was washed with water and dried over ...

example 2

Preparation of N-hydroxy-2-(((2-(6-methoxypyridin-3-yl)-4-morpholinothieno[3,2-d]pyrimidin-6-yl)methyl)(methyl)amino)pyrimidine-5-carboxamide methanesulfonate (Compound 2)

[0167]Method A: To a mixture of Compound 1 (300 mg, 0.59 mmol) and MeOH / Et2O (3 / 1, 40 mL) was added a solution of methanesulfonic acid (114 mg, 1.18 mmol) in MeOH (3 mL) at 0° C. The resulting mixture was stirred at 0° C. for 3 h. The precipitate was collected by filtration and washed with Et2O to afford Compound 2 as a white solid (260 mg, 73%).

[0168]Method B: To a suspension of Compound 1 (1.5 g, 2.95 mmol) in dichloromethane / MeOH (40 mL / 10 mL) was added methanesulfonic acid (341 mg, 3.55 mmol) in 2 mL MeOH at room temperature (15° C.) to form a clear solution. The reaction mixture was stirred at room temperature overnight. The reaction mixture was still clear. Ethyl acetate (40 mL) was added to the mixture and continued to stir for 3 h at room temperature. The resulting precipitate was collected by filtration to...

example 3

Preparation of N-hydroxy-2-(((2-(6-methoxypyridin-3-yl)-4-morpholinothieno[3,2-d]pyrimidin-6-yl)methyl)(methyl)amino)pyrimidine-5-carboxamide sodium salt (Compound 3)

[0170]To a suspension of Compound 1 (300 mg, 0.59 mmol) in methanol (30 mL) at 0° C. was added slowly t-BuONa (85 mg, 0.88 mmol). The resulting mixture was warmed to room temperature and continued to stir for 2 h. The reaction was concentrated and the residue was triturated and washed with ethanol followed by filtration to afford Compound 3 as a white solid (230 mg, 73%). m.p.: 178-183° C. LCMS: 509.3 [M+1]+. 1H NMR (400 MHz, DMSO-d6): δ 3.17 (s, 3H), 3.75 (s, 4H), 3.92 (s, 7H), 5.16 (s, 2H), 6.90 (d, J=8.4 Hz, 1H), 7.42 (s, 1H), 8.57 (d, J=8.0 Hz, 1H), 8.65 (s, 2H), 9.14 (s, 1H).

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Abstract

The invention provides a compound of Formula I,Pharmaceutical compositions comprising such compounds and the use of such compounds in the treatment of phosphoinositide 3-kinase related diseases and disorders such as cancer. The instant application further relates to the treatment of histone deacetylase related disorders and diseases related to both histone deacetylase and phosphoinositide 3-kinase.

Description

RELATED APPLICATIONS[0001]This application claims the benefit of U.S. Provisional Application No. 61 / 470,849, filed on Apr. 1, 2011 and 61 / 559,489, filed on Nov. 14, 2011. The entire teachings of the above applications are incorporated herein by reference.BACKGROUND OF THE INVENTION[0002]Phosphoinositides (PIs), which are phosphorylated derivatives of phosphatidylinositol, are essential in eukaryotic cells, regulating nuclear processes, cytoskeletal dynamics, signalling and membrane trafficking. Among the enzymes involved in PI metabolism, PI3-kinases (PI3K) have attracted special attention because of their oncogenic properties and potential as drug targets. PI3-kinases phosphorylate phosphatidylinositols or PIs at the 3-position of the inositol ring. (Lindmo et al. Journal of Cell Science 119, 605-614, 2006). The 3-phosphorylated phospholipids generated by PI3K activity bind to the pleckstrin homology (PH) domain of protein kinase B (PKB), causing translocation of PKB to the cell m...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07D495/04
CPCC07D495/04A61K31/166A61K31/4365C07D213/30C07D241/10A61P35/00A61P35/02A61P43/00A61K31/519
Inventor CAI, XIONGZHAI, HAIXIAOLAI, CHENGJUNGQIAN, CHANGGENGBAO, RUDI
Owner CURIS INC
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