Feed additive composition

a technology of additive composition and feed, which is applied in the direction of accessory food factors, animal feeding stuff, enzymes, etc., can solve the problems that the interaction between dfms and enzymes has never been fully understood, and achieve the effect of improving the performance of an animal

Inactive Publication Date: 2013-12-12
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0136]One advantage of the present invention is that the feed additive composition according to the present invention can avoid the negative effects of necrotic enteritis or can be used for improving the subject's resistance to necrotic enteritis.
[0137]Without wishing to be bound by theory, phytase catalyzes the sequential hydrolysis of phytate, a principal storage form of phosphorus in cereals and legumes, to less phosphorylated myo-inositol derivatives with concomitant release of inorganic phosphate. Hydrolysis of phytate causes a reduction of endogenous losses of amino acids to the intestinal lumen. A reduction of endogenous amino acid losses in the intestine reduces the availability of nitrogen for bacterial growth, which helps the activity of DFMs on inhibition of C. perfringens and other pathogenic bacteria.
[0138]Without wishing to be bound in theory proteases cause non-specific hydrolysis of dietary protein yielding a variety of polypeptides in the intestinal lumen. Animals finalise protein hydrolysis and absorb such amino acids. However, in the case of enteric pathogenic challenges, pathogenic bacteria may take advantage of higher peptide availability in the lumen of jejunum and ileum. DFMs inhibit the growth of entero-pathogens by for example competing for N sources, as well as by direct inhibition.
[0139]In combination these enzymes and DFMs surprisingly provide a significant improvement on the pathogen reduction and / or resistance to necrotic enteritis compared with other DFM and enzyme combinations and / or DFMs alone and / or enzyme(s) alone.Formulation of the DFM with the Enzymes
[0140]The DFM and the enzymes may be formulated in any suitable way to ensure that the formulation comprises viable DFMs and active enzymes.
[0141]In one embodiment the DFM and enzymes may be formulated as a liquid, a dry powder or a granule.

Problems solved by technology

Although combinations of DFMs with some enzymes have been contemplated, the interaction between DFMs and enzymes has never been fully understood.

Method used

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Examples

Experimental program
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Effect test

example 1

Materials and Methods

[0329]Three thousand six hundred one-day-old Cobb male chicks are purchased from a commercial hatchery. At study initiation, fifty males are allocated to each treatment pen by blocks. The study consisted of the following treatments (Table 1):

TABLE 1Experimental design of Example 1.perfringensAdditionalTreatmentChallengePhytase1enzyme2DFM31No500 FTU / kgNoneNone2Yes500 FTU / kgNoneNone3Yes500 FTU / kgProteaseNone(5000 u / kg)4Yes500 FTU / kgNoneEnviva Pro(7.5 × 104CFU / g)5Yes500 FTU / kgProteaseEnviva Pro(5000 u / kg)(7.5 × 104CFU / g)1Phytase from E. coli.2protease from Bacillus subtilis.3Enviva Pro ® is combination of Bacillus subtilis strains Bs2084, LSSAO1 and 15AP4, provided by Danisco A / S.

[0330]Bird weights by pen were recorded at study initiation, 23 d, 35 d, and termination (42 d). The pen was the unit of measure. Broiler diets were fed as crumbles (starter) or pellets (grower and finisher). Diets met or exceeded NRC standards (Table 2). The mixer was flushed to prevent c...

example 2

Materials and Methods

[0346]One digestibility trial with broiler chickens is conducted to determine the effects of protease and DFMs treatments on top of phytase on nutrient utilisation. The cages are housed in environmentally controlled rooms. The birds receive 20-hour fluorescent illumination and, are allowed free access to the diets and water. On day 1, a broiler live coccidiosis vaccine is given to all chicks orally. The study consists of the following treatments (Table 3).

TABLE 3Experimental design of Example 2.TreatmentPhytase1ProteaseDFM21500 FTU / kgNoneNone2500 FTU / kgProtease 13 (5000 u / kg)None3500 FTU / kgProtease 13 (5000 u / kg)None4500 FTU / kgProtease 24 (15000 u / kg)None5500 FTU / kgNoneEnviva Pro(1.5 × 105 CFU / g)6500 FTU / kgProtease 13 (5000 u / kg)Enviva Pro(1.5 × 105 CFU / g)7500 FTU / kgProtease 13 (5000 u / kg)Enviva Pro(1.5 × 105 CFU / g)8500 FTU / kgProtease 24 (15000 u / kg)Enviva Pro(1.5 × 105 CFU / g)1Phytase from E. coli provided by Danisco A / S.2Enviva Pro ® is combination of Bacillus ...

example 3

Materials and Methods

[0352]One digestibility trial with broiler chickens is conducted to determine the effects of phytase+protease and DFMs treatments on nutrient utilisation. The cages are housed in environmentally controlled rooms. The birds receive 20-hour fluorescent illumination and, allowed free access to the diets and water. On day 1, a broiler live coccidiosis vaccine is given to all chicks in a spray cabinet. The study consists of the following treatments (Table 5).

TABLE 5Experimental design of Example 3.TreatmentPhytase1Protease2DFM1NoneNone2500 FTU / kg5000 u / kg3NoneNoneEnviva Pro3 (1.5 × 105 CFU / g)4500 FTU / kg5000 u / kgEnviva Pro3 (1.5 × 105 CFU / g)5NoneNoneCalsporin4 (5.0 × 106 CFU / g)6500 FTU / kg5000 u / kgCalsporin4 (5.0 × 106 CFU / g)7NoneNoneGallipro Tect5 (8.0 × 105 CFU / g)8500 FTU / kg5000 u / kgGallipro Tect5 (8.0 × 105 CFU / g)1Phytase from E. coli.2Protease from Bacillus subtilis provided by Danisco A / S.3Enviva Pro ® is combination of Bacillus subtilis strains Bs2084, LSSAO1 and...

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Abstract

A feed additive composition comprising a direct fed microbial in combination with a protease, and a phytase, and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject, which method comprising administering to a subject a direct fed microbial in combination with a protease and a phytase.

Description

FIELD OF INVENTION[0001]The present invention relates to methods for improving feed compositions using a direct fed microbial in combination with a specific combination of enzymes, and to a feed additive composition comprising a direct fed microbial in combination with a specific combination of enzymes. The present invention further relates to uses and kits.BACKGROUND OF THE INVENTION[0002]Supplemental enzymes are used as additives to animal feed, particularly poultry and swine feeds, as a means to improve nutrient utilization and production performance characteristics. Enzyme blends are available to improve the nutritional value of diets containing soybean meal, animal protein meals, or high fibre food by-products.[0003]The concept of direct fed microbials (DFM) involves the feeding of beneficial microbes to animals, such as dairy cattle when they are under periods of stress (disease, ration changes, environmental or production challenges). Probiotics is another term for this categ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A23K1/00
CPCA23K1/009A61K35/741A61K35/742A61K35/744A61K35/745A61K35/747A61K38/465A61K38/48A23K10/18A23K20/189C12N9/50A61K2300/00A23K40/10A23K40/30A23K50/30A23K50/60A23K50/75A61P1/00A61P29/00A61P33/02C12N1/20C12N9/16C12Y301/03026C12N1/205C12R2001/125
Inventor MILLAN, LUIS FERNANDO ROMERO
Owner DUPONT NUTRITION BIOSCIENCES APS
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