Glucosyl stevia composition

Active Publication Date: 2014-08-14
PURECIRCLE SDN BHD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0020]The present invention is aimed to overcome the disadvantages of existing Stevia sweeteners. The invention describes a process for producing a high purity f

Problems solved by technology

However many artificial sweeteners such as dulcin, sodium cyclamate and saccharin were banned or restricted in some countries due to concerns on their safety.
However, even in a highly purified state, steviol glycosides still possess undesirable taste attributes such as bitterness, sweet aftertaste, licorice flavor, etc.
One of the main obstacles for the successful commercialization of stevia sweeteners are these undesirable taste attributes.
However in

Method used

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  • Glucosyl stevia composition
  • Glucosyl stevia composition
  • Glucosyl stevia composition

Examples

Experimental program
Comparison scheme
Effect test

Example

Example 1

Preparation of CGTase

[0072]A strain of Bacillus stearothermophilus St-100 was inoculated in 2,000 liters of sterilized culture medium containing 1.0% starch, 0.25% corn extract, 0.5% (NH4)2SO4, and 0.2% CaCO3 (pH 7.0-7.5) at 56° C. for 24 hrs with continuous aeration (2,000 L / min) and agitation (150 rpm). The obtained culture broth was filtered using Kerasep 0.1 μm ceramic membrane (Novasep, France) to separate the cells. The cell-free permeate was further concentrated 2-fold on Persep 10 kDa ultrafilters (Orelis, France). The activity of the enzyme was determined according to Hale, Rawlins (1951). A crude enzyme preparation with activity of about 2 unit / mL was obtained.

Example

Example 2

Preparation of β-Amylase

[0073]A strain of Bacillus polymyxa St-3504 was inoculated in 2,000 liters of sterilized culture medium containing 1.0% starch, 0.5% peptone, 0.5% corn extract, 0.5% NaCl, 0.02% MnSO4 and 0.1% CaCO3 (pH 7.0-7.5) at 32° C. for 24 hrs with continuous aeration (2,000 L / min) and agitation (150 rpm). The obtained culture broth was filtered using Kerasep 0.1 μm ceramic membrane (Novasep, France) to separate the cells. 10% of glucose was added to the cell-free permeate which was further concentrated on Persep 10 kDa ultrafilters (Orelis, France) and dried using Alpha 1-4 LSC freeze drier unit (Christ, Germany) to obtain a powder with 20,000 AUN / g activity. β-Amylase activity unit (1 AUN) was defined as the activity which liberates 100 mg of reducing sugar (expressed by dextrose equivalent) per minute under the following conditions: 1 mL of enzyme solution is mixed with 5 mL of 1.2% starch solution (pH 5.5, M / 20 Acetate Buffer) and kept for 20 min at 40° C.

Example

Example 3

Preparation of Short-Chain Glucosyl Stevia Composition

[0074]100 g of tapioca starch was suspended in 300 mL of water (pH 6.5). 2 KNU of α-amylase (Termamyl Classic, Novozymes, Denmark) and 30 units of CGTase obtained according to EXAMPLE 1 were added, and the liquefaction of starch was carried out at 80° C. for about one hour to dextrose equivalent about 15. The pH of reaction mixture was adjusted to pH 2.8 by hydrochloric acid and the mixture was boiled at 100° C. during 5 minutes to inactivate the enzymes. After cooling to 65° C., the pH was adjusted to pH 6.0 with sodium hydroxide solution. 100 g stevia extract produced by PureCircle (JiangXi) Co., Ltd. (China), containing stevioside 29.2%, Rebaudioside A 54.3%, Rebaudioside C 9.0%, Rebaudioside F (1.7%) and other glycosides amounting to total steviol glycosides content of about 96.4% was added to liquefied starch and stirred until a homogeneous solution was obtained. 200 units of CGTase was added to the solution and the...

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Abstract

Glucosyl stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using the starch as source of glucose residues. The short-chain glucosyl stevia compositions were purified to >95% content of total steviol glycosides. The compositions can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.

Description

RELATED APPLICATIONS[0001]This application is a continuation-in-part application of and claims the benefit of priority from:[0002]co-pending U.S. patent application Ser. No. 14 / 073,423, filed on Nov. 6, 2013, which is a continuation application of U.S. patent application Ser. No. 13 / 567,707, filed on Aug. 6, 2012 and issued as U.S. Pat. No. 8,647,844 on Feb. 11, 2014, which is a divisional application of U.S. patent application Ser. No. 13 / 029,263, filed on Feb. 17, 2011 and issued as U.S. Pat. No. 8,257,948 on Sep. 4, 2012; and[0003]co-pending U.S. patent application Ser. No. 13 / 589,754, filed on Aug. 20, 2012, which is a continuation of U.S. patent application Ser. No. 13 / 074,179, filed on Mar. 29, 2011 and issued as U.S. Pat. No. 8,318,459 on Nov. 27, 2012, which is a continuation-in-part application of U.S. patent application Ser. No. 13 / 029,263, filed on Feb. 17, 2011 and issued as U.S. Pat. No. 8,257,948 on Sep. 4, 2012.[0004]The contents of each of these applications are inco...

Claims

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Application Information

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IPC IPC(8): A23L1/221A23L27/10
CPCA23L1/221A23C9/1307A23L2/60A21D2/18A21D2/36A61K36/28C12P19/18C12P19/56C12P33/00A61K31/704A23L2/02C12P19/22C12Y204/01019A23L27/36A23L2/54Y02P20/52A23L27/33A23L33/105A23V2002/00
Inventor MARKOSYAN, AVETIK
Owner PURECIRCLE SDN BHD
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