Use of lipid conjugates in the treatment of inflammatory disorders
a technology of lipid conjugates and phospholipids, applied in the field of lipid conjugates and phospholipid conjugates, can solve the problems of lack of direction in the flow of blood, frequent emptying of vessels, and formation of small capillaries, and achieve the effect of inhibiting the development of hemangioma
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example 1
Control of Angiogenic Processes by Inhibition of Spla2 Activity
[0253]To comprehensively study the possible control of angiogenic processes by inhibition of sPLA2 activity, the effect of HyPE on HBMEC proliferation, migration and capillary formation was determined. As noted in the materials section, the medium used for cultivation of the endothelial cells is already supplemented with growth factors. To examine the effect of HyPE on growth factor-induced endothelial cell activation, the culture medium was further supplemented with bFGF, VEGF and OSM, which further increased HBMEC stimulation by about 50%, as shown below.
Effect of HyPE on HBMEC Proliferation
[0254]FIG. 1 shows that HyPE effectively inhibited HBMEC proliferation in a dose-dependent manner, whether the cells were grown in the control culture medium containing standard amount of growth factors, or stimulated with additional amount of VEGF, bFGF or OSM. At the same time, HyAc did not affect the cell proliferation. It should...
example 2
Invasive Cellular Proliferative Disorders
[0264]The Lipid-conjugates are effective therapy for cellular proliferative disorders, such as cancer. This is demonstrated in experiments 7.1-7.3 above and 8.1-8.8 below. The process of cancer spread entails multiple events, each of these is a worthy target for inhibitory drug action, including the rate of cell-proliferation, the rate of spread through blood vessels, the rate of invasiveness through contiguous and non-contiguous (metastases) tissues, and the rate of production of new blood vessels to supply the cancerous growth. Cancer cells frequently produce intracellular matrix tissue degrading enzymes which serve to enhance their invasive potential. Cancer is thus a multiphasic disease involving the process of tissue invasiveness, spread through tissue channels, angiogenesis and tumor vascularization. These latter processes depend upon the rates of proliferation of endothelial cells and smooth muscle cells.
[0265]Lipid-conjugates inhibit ...
example 3
Glycolipid Conjugates Modulate Chemokine and / or Cytokine Expression
[0279]The effects of the Lipid-conjugates were tested in the following cell lines: 16HBE, IB-3 and C-38 cells.
[0280]Studies of the pathogenesis of rheumatoid arthritis (RA) have revealed that both synovial fibroblasts and T cells participate in the perpetuation of joint inflammation as dynamic partners in a mutual activation feedback, via secretion of cytokines and chemokines that stimulate each other. IL-8 is frequently found at high levels in diseases associated with neutrophil. influx, and during the course of rheumatoid arthritis. IL-6 is frequently found at high levels in diseases associated with neutrophil. influx, and during the course of rheumatoid arthritis.
[0281]The cells were grown to confluency in 96 well plates, washed, and Lipid-conjugates (Compounds XXII, XXIII, and XXV) or sham were added to the cells, which were incubated at 37° C. for 30 minutes. Cells were washed, and in some groups, incubated with...
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