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Composition for Promoting Anti-Diabetic and Anti-Obesity Effects, Comprising Herbal Extract

a technology of antidiabetic and antiobesity effects, applied in the direction of medical preparations, plant ingredients, plant/algae/fungi/lichens ingredients, etc., can solve the problems of 20 to 30% of patients taking metformin side effects and loss of appetite, so as to improve the therapeutic effect of diabetes mellitus, improve the therapeutic effect, and reduce side effects

Inactive Publication Date: 2017-08-24
DONGGUK UNIV GYEONGJU CAMPUS IND ACAD COOPERATION FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is a pharmaceutical composition that uses an extract from Lonicera japonica, Scutellaria baicalensis and Houttuynia cordata as an active ingredient. Combining this extract with metformin, an anti-diabetic drug, improves its effectiveness and reduces side effects, making it useful for treating diabetes mellitus. The extract also has an inhibitory effect on fat accumulation, which makes it a potential treatment for obesity as well.

Problems solved by technology

However, it has been reported that 20 to 30% of patients taking metformin suffer side effects, such as loss of appetite, abdominal distension, nausea, and diarrhea.

Method used

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  • Composition for Promoting Anti-Diabetic and Anti-Obesity Effects, Comprising Herbal Extract
  • Composition for Promoting Anti-Diabetic and Anti-Obesity Effects, Comprising Herbal Extract
  • Composition for Promoting Anti-Diabetic and Anti-Obesity Effects, Comprising Herbal Extract

Examples

Experimental program
Comparison scheme
Effect test

example 1

ity Experiments for Lonicera japonica Extracts

[0112]100 μl of 3T3-L1 cells was aliquoted to each well of a 96-well plate at 3×103 cells / well and incubated in a CO2 incubator for 24 hours. Samples at various concentrations were added to each well and incubated for 24 hours, and thereafter 10 μl of EZ-Cytox was added to each well. After incubation for 2 hours in an incubator, the plate was shaken for 1 minute before measuring absorbance and then absorbance was measured at 450 nm using a 96-well plate reader. Cytotoxicity was measured according to extraction methods (water extract: GEH, 30% ethanol extract: GEH30, 100% ethanol extract: GEH100), whether metformin was co-administered, and concentration changes of Lonicera japonica extracts (20, 50, 100, 200 μg / ml).

[0113]As a result, as illustrated in FIGS. 1 to 3, cytotoxicity was not observed in all groups regardless of extraction method and whether single administration or co-administration with metformin was carried out. In addition, ...

example 2

nt of Changes in Intracellular Reactive Oxygen Species (ROS) Activity by Administration of Lonicera japonica Extracts

[0114]2 ml of HepG2 cells was aliquoted to each well of a 6-well plate at 3×105 cells / well and incubated in a CO2 incubator for 8 hours, and then the HepG2 cells were either treated with metformin alone or with metformin in combination with a Lonicera japonica extract and incubated for 6 hours, followed by cell harvesting. After centrifugation at 1200 g for 5 minutes, a supernatant was discarded, and the remaining HepG2 cells were treated with 5 μg / ml of DHR123, followed by incubation at 37° C. for 30 minutes. After additional centrifugation for 5 minutes, PBS washing was performed two times and filtration was performed. Intracellular reactive oxygen species activity was measured based on the value of fluorescence intensity obtained from FACS analysis.

[0115]As a result, as illustrated in FIG. 4, a metformin-administered group (Metformin) exhibited a tendency of decrea...

example 3

nt of DPPH Free Radical Scavenging Activity by Administration of Lonicera japonica Extracts

[0116]40 μl of a sample was mixed with 760 μl of 300 μM 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the mixture was incubated at 37° C. for 30 minutes, and then the mixture was aliquoted to each well of a 96-well plate in triplicate and absorbance was measured at 515 nm using a microplate reader. BHT was used as a positive control group. In Example 3, depending upon 3 extraction methods (water, 30% ethanol, and 100% ethanol extractions), the DPPH free radical scavenging capacity of a Lonicera japonica extract was measured and IC50 values were calculated.

[0117]As a result, BHT, a control group, showed a value of 113.85 μg / ml. In addition, when a Lonicera japonica water extract, a Lonicera japonica 30% ethanol extract, and a Lonicera japonica 100% ethanol extract were administered, as illustrated in the following Table 1, IC50 values were 143.36 μg / ml, 154.35 μg / ml, and 146.93 μg / ml, respectively, ...

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Abstract

The present invention relates to a composition for improving anti-diabetic and anti-obesity effects, including an extract extracted from any one selected from the group consisting of Lonicera japonica (Lonicerae Flos), Scutellaria baicalensis (Scutellariae Radix), and Houttuynia cordata (Houttuyniae Herba). According to the present invention, it was confirmed that co-administration of the extract of the present invention and metformin, a representative anti-diabetic drug, increases an anti-diabetic effect and reduces side effects caused by metformin, and at the same time, exhibits an anti-obesity effect by suppressing fat accumulation. Therefore, the composition of the present invention is expected to be effective in treatment of diabetes mellitus.

Description

TECHNICAL FIELD[0001]The present invention relates to a composition for improving anti-diabetic and anti-obesity effects including the extract of a crude drug, and more particularly, to a composition including an extract of Lonicera japonica (Lonicerae Flos), Scutellaria baicalensis (Scutellariae Radix), or Houttuynia cordata (Houttuyniae Herba), which is capable of improving the therapeutic effects of metformin as an anti-diabetic drug on diabetes mellitus and simultaneously treating obesity.BACKGROUND ART[0002]Diabetes mellitus is a disease characterized by high blood sugar, which is caused by absolute or relative insulin deficiency and insulin resistance in tissues, and metabolic disorders accompanying the same. Type 2 diabetes mellitus, which is increasing with the rise in obesity due to changes in dietary patterns and lifestyles as a result of development of human civilization, is attributed to insulin resistance considered as a major pathophysiological feature in type 2 diabet...

Claims

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Application Information

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IPC IPC(8): A61K36/78A61K36/35A61K31/155A61K36/539
CPCA61K36/78A61K31/155A61K36/35A61K36/539A61K2300/00
Inventor KIM, HOJUNCHIN, YOUNG-WONCHOI, HAN SEOK
Owner DONGGUK UNIV GYEONGJU CAMPUS IND ACAD COOPERATION FOUND
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