Composition for Promoting Anti-Diabetic and Anti-Obesity Effects, Comprising Herbal Extract
a technology of antidiabetic and antiobesity effects, applied in the direction of medical preparations, plant ingredients, plant/algae/fungi/lichens ingredients, etc., can solve the problems of 20 to 30% of patients taking metformin side effects and loss of appetite, so as to improve the therapeutic effect of diabetes mellitus, improve the therapeutic effect, and reduce side effects
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example 1
ity Experiments for Lonicera japonica Extracts
[0112]100 μl of 3T3-L1 cells was aliquoted to each well of a 96-well plate at 3×103 cells / well and incubated in a CO2 incubator for 24 hours. Samples at various concentrations were added to each well and incubated for 24 hours, and thereafter 10 μl of EZ-Cytox was added to each well. After incubation for 2 hours in an incubator, the plate was shaken for 1 minute before measuring absorbance and then absorbance was measured at 450 nm using a 96-well plate reader. Cytotoxicity was measured according to extraction methods (water extract: GEH, 30% ethanol extract: GEH30, 100% ethanol extract: GEH100), whether metformin was co-administered, and concentration changes of Lonicera japonica extracts (20, 50, 100, 200 μg / ml).
[0113]As a result, as illustrated in FIGS. 1 to 3, cytotoxicity was not observed in all groups regardless of extraction method and whether single administration or co-administration with metformin was carried out. In addition, ...
example 2
nt of Changes in Intracellular Reactive Oxygen Species (ROS) Activity by Administration of Lonicera japonica Extracts
[0114]2 ml of HepG2 cells was aliquoted to each well of a 6-well plate at 3×105 cells / well and incubated in a CO2 incubator for 8 hours, and then the HepG2 cells were either treated with metformin alone or with metformin in combination with a Lonicera japonica extract and incubated for 6 hours, followed by cell harvesting. After centrifugation at 1200 g for 5 minutes, a supernatant was discarded, and the remaining HepG2 cells were treated with 5 μg / ml of DHR123, followed by incubation at 37° C. for 30 minutes. After additional centrifugation for 5 minutes, PBS washing was performed two times and filtration was performed. Intracellular reactive oxygen species activity was measured based on the value of fluorescence intensity obtained from FACS analysis.
[0115]As a result, as illustrated in FIG. 4, a metformin-administered group (Metformin) exhibited a tendency of decrea...
example 3
nt of DPPH Free Radical Scavenging Activity by Administration of Lonicera japonica Extracts
[0116]40 μl of a sample was mixed with 760 μl of 300 μM 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the mixture was incubated at 37° C. for 30 minutes, and then the mixture was aliquoted to each well of a 96-well plate in triplicate and absorbance was measured at 515 nm using a microplate reader. BHT was used as a positive control group. In Example 3, depending upon 3 extraction methods (water, 30% ethanol, and 100% ethanol extractions), the DPPH free radical scavenging capacity of a Lonicera japonica extract was measured and IC50 values were calculated.
[0117]As a result, BHT, a control group, showed a value of 113.85 μg / ml. In addition, when a Lonicera japonica water extract, a Lonicera japonica 30% ethanol extract, and a Lonicera japonica 100% ethanol extract were administered, as illustrated in the following Table 1, IC50 values were 143.36 μg / ml, 154.35 μg / ml, and 146.93 μg / ml, respectively, ...
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