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Method for separating L-amino-acid oxidase from venin

A technology of amino acid and oxidase, which is applied in the field of separation of L-amino acid oxidase, can solve problems such as the influence of L-amino acid oxidase enzyme activity, and achieve the effects of easy promotion, simple method, and avoiding the influence of enzyme activity

Inactive Publication Date: 2007-11-14
SHANTOU UNIV MEDICAL COLLEGE
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the existing L-amino acid oxidase separation technology includes concentration and dialysis to change the buffer system, thereby affecting the enzymatic activity of L-amino acid oxidase, and to provide a kind of L-amino acid oxidase without concentration and dialysis to change the buffer system. -Amino acid oxidase separation method

Method used

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  • Method for separating L-amino-acid oxidase from venin
  • Method for separating L-amino-acid oxidase from venin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] 1. Heparin affinity chromatography

[0032] (1) Sample processing: Weigh 200 mg of Agkistrodon halys venom, dissolve it in 5 mL of 25 mmol / LTris-HCl buffer solution (pH 8.5), and centrifuge at 10,000 r / min for 10 min at 4°C. Take the supernatant.

[0033] (2) Column packing: the volume of the chromatographic column is 25ml, and the filler is Heparin-Sepharose Fast Flow. Mobile phase A liquid is 25mmol / L Tris-HCl buffer solution (pH8.5), and B liquid is A liquid containing 1mol / L NaCl. When the liquid A is fully balanced until the conductivity value does not change, the snake venom sample is applied.

[0034] (3) Elution: After 50 mL of solution A, 250 mL was eluted with a linear gradient from 0% B to 100% B, and then 50 mL of column was washed with 100% B; the flow rate was 2 mL / min.

[0035] (4) Collection and detection: collect the eluate with a step-by-step collector, collect 3mL in each tube, and detect the protein content under the light absorption of 280nm. Th...

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Abstract

It was involved in separating L-amino acid oxidase from the snake venom of Agkistrodonhalysussuriensis. Through two steps of colum chromatography (i.e. heparin fast flow and qsepherose fast flow) L-amino acid oxidase was separated and purified from the snake toxin of Gloydius ussuriensis. Not including transfer between concentrating and buffer system. It could avoid the influence of freezing and the pH value change to enzyme activity. The method was found to be simple, rapid and convenient. The ratio of recovery was high.

Description

technical field [0001] The invention relates to an L-amino acid oxidase, in particular to a method for separating the L-amino acid oxidase. Background technique [0002] L-amino acid oxidase can catalyze the deamination of L-amino acids to generate the corresponding α-keto acids, ammonia and hydrogen peroxide. In industrial production, L-amino acid oxidase is used to produce α-keto acid; it can degrade L-amino acid in the mixture of L-amino acid and D-amino acid, thereby separating D-amino acid. The L-amino acid oxidase can also be used to prepare an enzyme electrode for determining L-amino acid. Snake venom is one of the main sources of L-amino acid oxidase. The yellow color of snake venom is produced by L-amino acid oxidase. At present, a number of L-amino acid oxidases have been isolated from snake venom, and a large number of studies have been carried out on their physical and chemical properties and physiological activities. It is found that L-amino acid oxidase has ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02C12N9/04
Inventor 何韶衡魏继福
Owner SHANTOU UNIV MEDICAL COLLEGE
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