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Process for producing bata-mannanase fermented by pseudo honey agaric and application thereof

A technology of mannanase and Pseudo Armillaria is applied in the directions of microorganism-based methods, biochemical equipment and methods, enzymes, etc., and achieves broad application prospects and good thermal stability.

Inactive Publication Date: 2008-08-13
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no relevant reports on the production of β-mannanase by Armillaria pseudoarmilla.

Method used

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  • Process for producing bata-mannanase fermented by pseudo honey agaric and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Armillariella tabescens AS5.92 CGMCC. The Pseudoarmillaria can secrete β-mannanase, β-mannanase has good heat resistance, the working temperature range is 30-75°C, and the optimum reaction temperature is 60°C; the reaction pH is 3-8, and the optimum pH is 5 .5~6.0. The method for fermenting and producing β-mannanase by Armillaria pseudoarmilla comprises the following steps:

[0023] (1) Qualitative detection of β-mannanase produced by Armillaria armillaria by transparent hydrolysis circle method

[0024] The slant bacteria were inoculated in the solid medium containing 0.1% Quliben blue, and the formula of the plate medium for producing transparent hydrolysis circle was calculated by mass percentage: 25% of potato juice, 1% of peptone, 2% of konjac powder, KH 2 PO 4 0.3%, MgSO 4 0.15%, vitamin B 1 0.01%. After static culture at 26.5°C for 8 days, a transparent hydrolysis circle can be seen around the bacteria.

[0025] (2) After the Pseudoarmillaria cultured on t...

Embodiment 2

[0033] The method for fermenting and producing β-mannanase by Armillaria pseudoarmilla comprises the following steps:

[0034] (1) Qualitative detection of β-mannanase produced by Armillaria armillaria by transparent hydrolysis circle method

[0035] The slant bacteria were inoculated in the solid medium containing 0.5% Quliben blue, and the formula of the plate medium for producing transparent hydrolysis circle was calculated by mass percentage: 10% of potato juice, 0.5% of peptone, 3% of konjac powder, KH 2 PO 4 0.5%, MgSO 4 0.1%, vitamin B 1 0.02%. After static culture at 26.5°C for 5 days, a transparent hydrolysis circle can be seen around the bacteria.

[0036] (2) After the Pseudoarmillaria cultured on the slant is subjected to primary liquid culture, secondary liquid culture, and tertiary liquid culture, it can be fermented to produce β-mannanase.

[0037] 1) Inclined surface culture: The formula of the medium is: 10% potato juice, 0.5% peptone, 3% konjac powder, K...

Embodiment 3

[0041] The method for fermenting and producing β-mannanase by Armillaria pseudoarmilla comprises the following steps:

[0042] (1) Qualitative detection of β-mannanase produced by Armillaria armillaria by transparent hydrolysis circle method

[0043] The slant bacteria were inoculated in the solid medium containing 0.3% Quliben blue, and the formula of the plate medium for producing a transparent hydrolysis circle was calculated by mass percentage: 40% of potato juice, 2% of peptone, 0.5% of konjac powder, KH 2 PO 4 0.1%, MgSO 4 0.5%, vitamin B 1 0.05%. After static culture at 26.5°C for 15 days, a transparent hydrolysis circle can be seen around the bacteria.

[0044] (2) After the Pseudoarmillaria cultured on the slant is subjected to primary liquid culture, secondary liquid culture, and tertiary liquid culture, it can be fermented to produce β-mannanase.

[0045] 1) Incline culture: The formula of the culture medium is: 40% of potato juice, 2% of peptone, 0.5% of konja...

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Abstract

The invention discloses a false armillaria mellea fermenting beta-mannase method and its application. Its qualitative detection and fermenting technique includes the following steps: inoculating incline fungus on solid medium with 0.1-0.5% Quli-blue to see transparent hydrolysis ring after 5-15 days; fermenting beta-mannase by the first, second, and third grade liquid culture. The technique is simple. The cost is low. And the application is high.

Description

technical field [0001] The invention relates to the technical field of biological fermentation engineering, in particular to a method for producing β-mannanase induced by Armillaria armillaria and its application in producing β-mannanase. Background technique [0002] Pseudoarmillaria can be induced to produce acidic β-mannanase. β-mannanase can decompose β-mannan (such as contained in konjac powder) to form mannan oligosaccharides. These oligosaccharides can eliminate the anti-nutritional effect of β-mannan in feed and improve the nutrition of feed At the same time, it can also promote the growth of beneficial bacteria Bifidobacteria in the human intestinal tract, regulate the immune system, etc. Therefore, acidic β-mannanase has broad application prospects in feed and food additives. The acidic β-mannanase produced by Armillaria armillaria has good prospects in application. At present, my country's health food market has developed into a large market with an annual sales ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/24C12N9/42C12N1/20C12N1/14C12R1/645
Inventor 姚冬生黄小葵刘大岭谢春芳
Owner JINAN UNIVERSITY
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