Method for preparing carbon nano-tube/poly L-cysteine composite modified glassy carbon electrode

Abstract

The invention relates to a manufacture method for carbon nm tube / poly L-cysteine compound modification glass carbon electrode. The process includes the following steps: purifying the multi-wall carbon nm tube, separating into N, N-dimethyl formamide solution, dropping the solution onto the surface of glass carbon electrode, drying under infrared light, locating the electrode into dilute hydrochloric acid containing L-cysteine to take electric polymerizing; taking out the electrode compounding L-cysteine to wash and gain carbon nm tube / poly L-cysteine compound modification glass carbon electrode. The method is simple, and low cost. The product has high reliability and good repeatability. It has long useful life and could be used in electrochemistry rapidly testing for uric acid and ascorbic acid in blood serum.

Description

Technical field [0001] The invention relates to a preparation method of a carbon nanotube / poly-L-cysteine ​​composite modified glassy carbon electrode for realizing simultaneous and rapid determination of uric acid, dopamine and ascorbic acid in serum. Background technique [0002] Uric acid is the main product of purine metabolism. Abnormal uric acid concentration in human body fluids can cause many diseases, such as gout and hyperuricemia. Excessive blood uric acid concentration can cause renal failure and cardiovascular disease. Therefore, the rapid and accurate determination of the concentration of uric acid in body fluids or blood is of great significance in biochemical and clinical diagnosis. Nowadays, enzyme-linked colorimetry is often used in clinics. Its advantage is high selectivity, but it is affected by enzyme activity, it has poor stability, expensive equipment, and low sensitivity. The electrochemical determination method has attracted widespread attention due to it...

AI Technical Summary

Problems solved by technology

However, the current peak resolution of the three existing methods is limited, the sensitivity is not high, the electrode preparation method is cumbersome, the electrode modification materials are expensive, not easy to obtain, and the biocompatibility is not good

So far, there has been no literature report on the modification of multi-walled carbon nanotubes and L-cysteine ​​to glassy carbon electrodes to make composite modified electrodes for the simultaneous determination of uric acid, dopamine, and ascorbic acid.

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