Skin whitening composition with synergic whitening effect
A technology of skin whitening and composition, which is applied in the field of skin whitening cosmetic composition, and can solve the problems of excessive suppression of melanin, impossibility, low necessary effect, etc.
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Embodiment 1 and 2
[0023] Preparation of skin whitening compositions (whitening energy complexes 1 and 2)
[0024] "Polyol-soluble licorice extract P-T (40)" was purchased as the licorice extract containing more than 35% glabridin, produced by Maruzen Pharmaceuticals Co. Ltd (Japan).
[0025] Red ginseng extract was prepared according to the following procedure, enzymatic hydrolysis of 6-year-old red ginseng extract produced by Bioland (Korea).
[0026] >
[0027] Dissolve 10 g of red ginseng extract in 100 ml of citrate buffer (pH 5.5), add 1 g of naringinase from Penicillium sp., and then stir the mixture in a water bath at 40°C for 48 Hour. When the substance disappeared and was regularly confirmed by thin-layer chromatography, the mixture was hydrothermally heated for 10 minutes to terminate the reaction, and then extracted and concentrated three times with the same ether. The product was analyzed by silica gel column chromatography (chloroform:methanol=9:1) to obtain a red ginseng extra...
experiment example 1
[0032] Measuring the effect of inhibiting melanin formation
[0033] HM3KO cells (Y.Funasaka, Department of Dermatology, Kobe University School of Medicine, 5-1 Kusunoki-cho7-chrome, Chuo-ku, Kobe 650, Japan), which are human melanoma cells, were placed in a medium containing 10% fetal bovine serum. Minimum Essential Medium (MEM) at 37°C and 5% CO 2 cultivated under conditions. Smear the cultured cells on the bottom of a 75-well flask, the number of cells is 3×10 5 per well and left overnight to allow the cells to adhere to the wall. After confirmation, each medium was changed to a new medium containing 10 ppm of the experimental sample.
[0034] The experimental samples used are shown in Table 1 below.
[0035] The medium containing DMSO (dimethyl sulfoxide) was used in the control group.
[0036] To compare the effects of each component, experiments were conducted with each component, namely, licorice extract, kojic acid, arbutin, vitamin A, vitamin C, red ginseng extr...
experiment example 2
[0044] skin whitening effect on human skin
[0045] Adhere an opaque adhesive tape with 6 holes with a diameter of 1.5 cm to the upper arms of each of 10 healthy men, and use ultraviolet light with an intensity of 1.5-2 times the minimum erythema dose (Minimal Erythema Dose) for each subject. Light beam (UV beam, UVB) is irradiated to induce skin melanin deposition. Then smear the test sample, and after 2 months, measure the depth (darkness) of the skin using a colorimeter.
[0046] The experimental samples were prepared according to the composition in Table 2, and the formulations of the examples and comparative formulations in Table 2 were applied twice a day (once in the morning and once in the evening).
[0047]
[0048] The formulation is an O / W (oil-in-water) lotion, and the unit is % by weight. The prepared formulations had a viscosity of 7000-11000 centipoise, measured on the second day after each formulation was placed in a constant temperature bath at 30°C, and ...
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