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Process for producing cytidine 5'-diphosphate choline

A technology for phosphorylcholine and choline, which is applied in the field of preparation of cytidine 5′-diphosphocholine, and can solve problems such as being not known by people

Inactive Publication Date: 2009-07-15
KYOWA HAKKO BIO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, methods utilizing uracil, which is a less expensive substrate, have not been understood so far

Method used

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Examples

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Embodiment 1

[0102] The plasmid pCKG55 recombined with CCT gene and CKI gene from yeast and PyrG gene from PyrG gene was transferred into Escherichia coli MM294 strain, and the Escherichia coli MM294 / pCKG55 strain obtained by transformation (JP-P-5-276974, FERM BP-3717) was inoculated in 200 ml of L medium containing 50 mg / L ampicillin [containing 10 g / L bactotryptone (manufactured by Difco), 5 g / L yeast extract (manufactured by Difco), 5 g / L sodium chloride, pH adjusted to 7.2] In a 2L Erlenmeyer flask with a stopper, cultivate for 24 hours at 25° C. with rotary shaking at 220 rpm. Take 20ml of this culture solution and inoculate it into 2.5L containing 5g / L glucose (separately sterilized), 5g / L peptone (manufactured by Jidong Pharmaceutical Industry Co., Ltd.), 6g / L disodium phosphate, 3g / L potassium dihydrogen phosphate, 1g / L L Ammonium Chloride, 250mg / L Magnesium Sulfate Heptahydrate (separately sterilized) and 4mg / L Vitamin B 1 (Separately sterilized) in a 5L culture tank of liquid c...

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PUM

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Abstract

The present invention relates to a process for producing CDP-choline, which comprises contacting a biocatalyst having the activity to form CDP-choline from choline or phosphorylcholine, or a salt thereof, and uracil with choline or phosphorylcholine, or a salt thereof, and uracil in an aqueous medium, allowing CDP-choline to form and accumulate in the aqueous medium, and recovering CDP-choline from the aqueous medium.

Description

technical field [0001] The invention relates to a preparation method of cytidine 5'-diphosphocholine. Background technique [0002] Cytidine 5′-diphosphocholine (hereinafter abbreviated as CDP-choline) is a biosynthetic intermediate of phosphatidylcholine (lecithin) of phospholipids, used for the treatment of head trauma and brain surgery Disorders of consciousness, Parkinson's disease, and stroke hemiplegia, etc. [0003] The known preparation method of CDP-choline has chemical synthesis method, utilizes microbial mycelium to pass through cytidine 5'-triphosphate (hereinafter abbreviated as CTP), cytidine 5'-diphosphate (hereinafter abbreviated as CDP) or The preparation methods of cytidine 5'-monophosphate (hereinafter abbreviated as CMP) etc. (Japanese Patent Publication 48-2358, Patent Publication 48-40758, Patent Publication 48-2359), but no matter which method has low yield, Raw material prices are high. [0004] Among the production methods utilizing microorganisms...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/30C12N15/09C12R1/19C12R1/15
CPCC12P19/30
Inventor 桥本信一小田秀树
Owner KYOWA HAKKO BIO CO LTD
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