Quick detection paper card for pesticide residue in vegetable and preparation method therof

A pesticide residue and vegetable technology, which is applied in the field of vegetable pesticide residue quick test paper card and its preparation field

Inactive Publication Date: 2009-07-29
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this quick test card must be equipped with a pesticide residue quick tester to detect it, and this quick test card can only detect organophosphorus pesticides

Method used

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  • Quick detection paper card for pesticide residue in vegetable and preparation method therof
  • Quick detection paper card for pesticide residue in vegetable and preparation method therof
  • Quick detection paper card for pesticide residue in vegetable and preparation method therof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1, the screening of enzyme source

[0036] Using horse serum, human serum, bovine liver, bee head, housefly head and other animal organs or serum as materials, extract and screen the highly sensitive enzyme source-acetylcholinesterase according to conventional methods. Mash frozen horse serum, human serum, bovine liver, bee brain, and housefly brain respectively, add distilled water at a ratio of 1:9 (W / V), homogenate for 3 minutes, and centrifuge at 2000-3000r / min for 10 minutes , the supernatant was filtered through filter paper, freeze-dried, divided into equipment, and stored at -20°C. It was the enzyme source.

[0037] 1) Reagent preparation:

[0038] Enzyme liquid preparation: Dilute freeze-dried horse serum enzyme powder, serum enzyme powder, bovine liver enzyme powder, bee brain enzyme powder, and housefly brain enzyme powder with PH8. .

[0039] Pesticide concentration: 0.8ppm for methamidophos and 0.002ppm for carbofuran.

[0040] Substrate: Thi...

Embodiment 2

[0047] Embodiment 2: the selection of enzyme concentration

[0048] 1) Enzyme preparation: Dilute the freeze-dried human serum enzyme powder of the present invention with pH 8.0 and 0.1M phosphate buffer to 0.5, 0.75, 1.0, 1.25, and 1.5 units respectively. Add one drop (about 30 μl) onto a qualitative filter paper with a diameter of 1.0 cm to make an enzyme tablet.

[0049] 2) Substrate: Mix 0.0448g potassium ferricyanide (red blood salt) and 0.0715g potassium ferrocyanide (yellow blood salt), add 100ml distilled water (PH5.5) after mixing, add one drop (about 30μl) in side becomes 1.0cm 2 On the qualitative filter paper, add dropwise 30μl indole acetate 180mg / 10ml acetone solution to make a substrate sheet.

[0050] 3) The concentration of methamidophos is 0.1 mg / μl.

[0051] 4) Use distilled water for comparison

[0052] The results of the reaction of five kinds of enzyme concentration enzyme tablets with the pesticide methamidophos are shown in Table 3

[0053] Table 5...

Embodiment 3

[0060] Embodiment 3: the selection of optimal substrate concentration

[0061] 1) Preparation of substrate sheet: Mix 0.0448g potassium ferricyanide (red blood salt) and 0.0715g potassium ferrocyanide (yellow blood salt) into 100ml distilled water, take a drop of the mixture (about 30μl pH5.5) first Add dropwise on 3 pieces with a side length of 1.0cm 2 Qualitative filter paper, and then put a drop (about 30μl) of indole acetate 180mg / 10ml acetone solution, indole acetate 270mg / 10ml acetone solution, indole acetate 360mg / 10ml acetone solution on three pieces of qualitative filter paper On the surface, dry at room temperature for use as a substrate sheet.

[0062] 2) Preparation of enzyme tablets: Dilute the frozen enzyme powder extracted from the serum with pH 8.0 and 0.1M phosphate buffer to 0.5 and 0.75 units respectively. Add it dropwise on a qualitative filter paper with a diameter of 1.0 cm to make an enzyme tablet.

[0063] The results of reacting 0.5 units and 0.75 u...

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Abstract

The invention provides a vegetable pesticide residue rapid detection paper card and its preparation method. The rapid detection paper card of the present invention uses acetylcholinesterase enzyme tablets extracted from human serum, which can detect organophosphorus and carbamate pesticides. The sensitivity reaches 0.001-1.2mg / kg, which is a highly sensitive enzyme source. The present invention is also an enzyme source with good thermal stability, especially after adding stabilizer gelatin or trehalose to the enzyme liquid, it can be used at about 90°C , Enzyme activity can be maintained for 7-8 hours. The rapid test paper card prepared by the present invention can detect common pesticides on vegetables, and the operation is simple and fast. It can clearly determine whether the residual pesticides on edible vegetables exceed the standard without the help of a residual pesticide detector, and only 12 minute. With low cost, it is not only suitable for home testing, but also brings great convenience to market supervision, rapid screening and monitoring of field vegetables, health and epidemic prevention stations, agricultural plant inspection stations and other units for the detection of vegetable pesticide residues.

Description

technical field [0001] The invention belongs to the analysis of pesticide residues, in particular, the invention relates to a rapid detection paper card for pesticide residues in vegetables and a preparation method thereof Background technique [0002] my country is a large agricultural country, with abundant crops. In addition to food crops, oil crops, fiber crops, sugar crops, melons and fruits, and medicinal tobacco, there are also large areas of fruits, vegetables, pastures, and forests. Therefore, China is also a big country in the application of pesticides. The invention and use of pesticides have undoubtedly greatly improved agricultural productivity, but the annual spraying of chemical pesticides to prevent and control pests and diseases is still an indispensable and necessary measure. With the improvement of people's quality of life, the demand for safe, nutritious, and pollution-free vegetables and fruits is becoming stronger and stronger. At present, the amount o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N33/52
Inventor 吴虹戴小杰
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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