Method for extracting flavone substance from persimmon leaf by double water-phase extraction

A technology of persimmon leaf flavonoids and two-phase water, which is applied in the direction of solid solvent extraction, etc., can solve the problems of low extraction rate and cumbersome operation, and achieve the effect of simple method, quick effect and easy mastering

Inactive Publication Date: 2009-11-18
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all have the following problems: the operation is loaded down with trivial details, and the extraction rate is low

Method used

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  • Method for extracting flavone substance from persimmon leaf by double water-phase extraction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Solid-liquid leaching

[0034] Weigh 20 g of persimmon leaves that have been washed, removed, dried, and crushed, add 300 mL of 50% ethanol for reflux extraction for 3 hours, recover the solvent by distillation, filter the remaining extract to remove impurities, and obtain 153 g of filtrate.

[0035] (2) Aqueous two-phase extraction

[0036] Get above-mentioned filtrate, add 20g polyethylene glycol, 35g (NH 4 ) 2 SO 4 , 100g of water, and then add 5g of inorganic salt NaCl, mix well, and separate phases at 15°C for 2h to 6h.

[0037] (3) Butyl acetate or ethyl acetate stripping

[0038] Take out 124mL of the upper phase rich in polyethylene glycol polymer, add 50mL of water, 80mL of butyl acetate or ethyl acetate and 3g of NaCl, mix thoroughly, and let the system stand at room temperature for 1h-3h to separate phases completely into upper and lower phases. A two-phase, butyl acetate phase containing high amounts of flavonoids is for further processing.

[0039...

Embodiment 2

[0042] (1) Solid-liquid leaching

[0043] Weigh 20 g of persimmon leaves that have been cleaned, dried, and crushed, add 400 mL of 70% ethanol for reflux extraction for 5 h, recover the solvent by distillation, filter the remaining extract to remove impurities, and obtain 190 g of filtrate.

[0044] (2) Aqueous two-phase extraction

[0045] Get above-mentioned filtrate, add 25g polyethylene glycol, 45g (NH 4 ) 2 SO 4 , 95g of water, and then add 2g of NaCl, mix well, and separate the phases at 25°C for 2h to 6h.

[0046] (3) Butyl acetate or ethyl acetate stripping

[0047] Take out 186mL of the upper phase rich in polyethylene glycol polymer, add 75mL of water, 113mL of butyl acetate, 3g of NaCl, mix well, and let the system stand at room temperature for phase separation for 1h to 3h until it is completely divided into upper and lower phases. The butyl acetate phase, which contains high amounts of flavonoids, is for further processing.

[0048] (4) vacuum distillation, ...

Embodiment 3

[0051] (1) Solid-liquid leaching

[0052] Weigh 20 g of persimmon leaves that have been cleaned, dried, and crushed, add 500 mL of 60% ethanol for reflux extraction for 8 hours, recover the solvent by distillation, filter the remaining extract to remove impurities, and obtain 389 g of filtrate.

[0053] (2) Aqueous two-phase extraction

[0054] Get above-mentioned filtrate, add 35g polyethylene glycol, 65g (NH 4 ) 2 SO 4 , 125g of water, and then add 3g of inorganic salt NaCl, mix well, and separate phases at 30°C for 2h to 6h.

[0055] (3) Butyl acetate or ethyl acetate stripping

[0056] Take out 221mL of the upper phase rich in polyethylene glycol polymer, add 85mL of water, 126mL of butyl acetate, 3g of NaCl, mix thoroughly, and let the system stand at room temperature for phase separation for 1h~3h until it is completely divided into upper and lower phases. The butyl acetate phase, which contains high amounts of flavonoids, is for further processing.

[0057] (4) va...

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Abstract

The invention relates to a method for extracting flavonoids from persimmon leaves by aqueous two-phase extraction. The technical feature of the invention is: drying and pulverizing the persimmon leaves, adding ethanol solution, leaching with solid and liquid, filtering and removing impurities to obtain the rough extract of persimmon leaves. Take the above crude persimmon leaf extract, add PEG polymer and ammonium sulfate two-phase system, mix well, and divide into upper and lower two phases. Take out the upper phase rich in polyethylene glycol polymer, add water, acetates and inorganic salts, and mix well. Taking out the acetate phase containing a large amount of flavonoids, performing vacuum distillation on it, concentrating the solution, and then vacuum-drying the concentrated solution to obtain solid persimmon leaf flavonoids. The beneficial effect of the invention is that the extraction rate of flavonoids from persimmon leaves is improved, high-quality flavonoids can be obtained, and the method itself is economical and easy to operate.

Description

technical field [0001] The invention relates to a method for extracting flavonoids from persimmon leaves by aqueous two-phase extraction. Background technique: [0002] Persimmon leaves are the fresh or dried leaves of the persimmon tree. As a medicine, it was first recorded in "Southern Yunnan Materia Medica" in the Ming Dynasty, saying: "Applying ecthyma sores through frost leaves". In the future, there will be many records in various herbal medicines and medical (medicine) books. Its taste is bitter, sour, astringent, and cool in nature. It has the effects of clearing lung and relieving cough, cooling blood to stop bleeding, promoting blood circulation and removing blood stasis, and lowering blood pressure. It is mainly used for cough, asthma, emphysema, various internal bleeding, high blood pressure and other diseases. The main active ingredients in persimmon leaves are flavonoids. Flavonoids that have been found so far include astragalus, isoquercetin, kaempferol, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D11/02
Inventor 岳红赵晓莉翟文俊
Owner NORTHWESTERN POLYTECHNICAL UNIV
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