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Bone repairing material and its prepn process and use

A technology for bone repair and application, which is applied in the field of bone repair materials and their preparation, and can solve the problems of hardness, strength, and easy shaping.

Active Publication Date: 2010-06-30
高心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a bone repair material, which overcomes the problems of existing artificial synthetic materials in terms of hardness, strength and ease of shaping

Method used

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  • Bone repairing material and its prepn process and use
  • Bone repairing material and its prepn process and use
  • Bone repairing material and its prepn process and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Preparation of Type I Collagen

[0070] Take fresh beef tendon, remove fat and fascia, etc., chop and weigh, soak in sodium carbonate solution for 2 hours, and rinse with distilled water. Use Tris-HCl buffer solution (pH 7.5) containing 1M NaCl to remove impurities. Digest and hydrolyze with 0.3% acetate enzyme solution (containing ficin or pepsin) for 48-72 hours. The absorbance of the extract was measured every 2 hours, and the digestion curve was drawn. High-speed refrigerated centrifugation, 200rpm / 20min, -4 ~ 4 ℃, take the supernatant, this is the collagen crude extract. with 1% H 2 o 2 The solution works for 4 hours. Salt out with NaCl solution for 12-18 hours, soak in Tris (pH 7.0) for 24 hours. After dialysis for 6 hours, the concentration of the dialysate NaCl solution decreased, and finally dialyzed with distilled water for 3 days. Freeze-dry and store at -20°C for later use.

Embodiment 2

[0072] Preparation of collagen-encapsulated bone repair materials

[0073] ① Mix collagen with 0.1% acetic acid to make 0.2% collagen stock solution;

[0074]②Continue to dilute the collagen stock solution with 0.1% acetic acid to make 0.01%-0.1% collagen;

[0075] ③ Dilute the collagen solution with 1mol / L NaHCO 3 Adjust the pH value to neutral, add calcined bone powder with a diameter of 0.05-2.5, so that the volume-to-weight ratio of the collagen solution to the bone powder is 5:1-20:1;

[0076] ④ Place the bone meal prepared in ③ in a water bath at 30-80°C for 4-6 hours. Centrifuge at 1500 rpm for 6 minutes, discard the supernatant, and take the precipitate;

[0077] ⑤The preparation in ④ was vacuum-dried and irradiated under ultraviolet light for 40 minutes. Turn over the particles several times during the period;

[0078] ⑥Observe the surface package under the electron microscope;

[0079] ⑦ Select the group with the best effect, fumigate with ethylene oxide, and s...

Embodiment 3

[0081] Preparation of chitosan-encapsulated bone repair materials

[0082] ① Dissolve bone powder in ethanol to make a 5% suspension, referred to as liquid A;

[0083] ②Weigh the chitosan according to the weight ratio of bone powder and chitosan of 4:1-1:3, dissolve the powder in 2% acetic acid aqueous solution, stir continuously for 5 hours, and filter to obtain completely transparent 3% chitosan solution;

[0084] ③ Pour the prepared 10% phosphoric acid aqueous solution into the chitosan solution, stir fully to obtain liquid B, and carry out the whole reaction at room temperature.

[0085] ④ Slowly drop liquid B into liquid A under vigorous stirring. During this process, the pH value is kept at about 10, and the dropping speed is 4ml / min. After the dropwise addition is completed, continue stirring for 24 hours. The obtained material is aged at room temperature for 1 day, and the precipitate is filtered. Wash and disinfect for later use.

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Abstract

The present invention discloses one kind of bone repairing material capable of inducing bone regeneration and its preparation process. The bone defect repairing material is composition of calcined bone powder of different sizes and bioabsorbable polymer material, the calcined bone powder has sizes of 0.05-2.5 mm; while the bioabsorbable polymer material is collagen, polyester, chitosan, etc. The bone defect repairing material has the preparation process includes the steps of mixing bone powder and bioabsorbable polymer material in acetic acid or NaHCO2 solution, ultraviolet irradiation of themixture and fumigating with ethylene oxide. The bone defect repairing material possesses obvious effect of inducing bone healing, high biocompatibility, certain hardness and strength and other advantages.

Description

technical field [0001] The invention discloses a bone repair material for guiding bone regeneration and a preparation method thereof, which is used for repairing bone defects and belongs to the technical field of biomaterial preparation. Background technique [0002] With the progress of modernization and the improvement of living standards, the pace of life is gradually accelerating, and diseases causing bone defects are on the rise. Bone defects caused by various lesion treatments such as trauma, inflammation, and bone tumor removal are often encountered clinically. [0003] It has always been a difficult problem in the field of orthopedic repair that the bone defect cannot heal after reaching a certain area. Bone grafting is the most effective measure to treat bone defects. Research on bio-substitute materials for bone grafting has been a long-term key research topic in the Department of Orthopedics. For centuries, people have tried a variety of different repair materia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40
Inventor 高心何钟勤
Owner 高心
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