Liquid for testing hydrogen sulfide and its colorimetric testing tube

A technology of hydrogen sulfide test and test liquid, which is applied in the direction of measuring device, analysis through chemical reaction of materials, and material analysis through observation of the influence on chemical indicators, etc., which can solve problems not including sterilization preparation procedures and post-treatment Program, hydrogen sulfide test test method is low in sensitivity, prone to false negative or weak positive reactions, etc., to achieve the effect of convenient use, accurate and reliable test results, and high practical value

Inactive Publication Date: 2007-09-05
白莉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. The sensitivity of the hydrogen sulfide test method is low, and the generated hydrogen sulfide must reach a relatively high concentration to form a black precipitate, and then judge
[0005] 2. This test method has strict requirements on the professional skills of operators, and it must be operated under strict sterile environmental conditions. There are many influencing factors involved in the operation process, and false negative or weak positive reactions are prone to occur.
[0006] 3. The test method takes a long time. Generally, the test time only takes 24 to 48 hours, not including the disinfection of the utensils, the sterilization preparation procedures and the post-processing procedures.

Method used

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  • Liquid for testing hydrogen sulfide and its colorimetric testing tube

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Embodiment 1

[0029] The formula of the hydrogen sulfide test test solution is: taking unit volume as an example, it is prepared by mixing 1 part of 2% p-aminodimethylaniline hydrochloride, 10 parts of 6% sulfuric acid solution and 4 parts of 10% ferric chloride solution .

[0030] Implementation method of hydrogen sulfide test colorimetric test tube: see Figure 1, in which 1 is a vacuum glass tube with equal cross-section, 2 is a capillary section, and 3 is a hydrogen sulfide test test solution. h 1 is the height of the test liquid, H 2 For the empty height of the equal-section vacuum tube, the outer diameter of the medium-section vacuum glass tube of this embodiment is 6 mm, the total height is 13.5 cm, and the height H of the test liquid in the glass tube is 1 is 2 cm, and the height of the space is H 2 is 10 cm, and the capillary segment length is 1.5 cm.

[0031] The hydrogen sulfide test test solution formula in the test tube of this embodiment is: taking unit volume as an example...

Embodiment 2

[0033] The formula of the hydrogen sulfide test test solution is: taking unit volume as an example, it is prepared by mixing 2 parts of 2% p-aminodimethylaniline sulfate, 25 parts of 6% sulfuric acid solution and 6 parts of 10% ferric chloride solution.

[0034] Implementation method of hydrogen sulfide test colorimetric test tube: see Figure 1, in which 1 is a vacuum glass tube with equal cross-section, 2 is a capillary section, and 3 is a hydrogen sulfide test test solution. h 1 is the height of the test liquid, H 2 For the empty height of the equal-section vacuum tube, the outer diameter of the medium-section vacuum glass tube of this embodiment is 7.5 mm, the total height is 12 cm, and the height H of the test liquid in the glass tube is 1 is 1 cm, and the height of the space is H 2 is 9 cm, and the capillary segment length is 2 cm.

[0035] The formula of the hydrogen sulfide test test solution in the test tube of this embodiment is: composed of 2 parts of 2% p-aminodi...

Embodiment 3

[0037] The formula of hydrogen sulfide test test solution is: taking unit volume as an example, it is prepared by mixing 1.5 parts of 2% p-aminodimethylaniline sulfate, 18 parts of 6% sulfuric acid solution and 5 parts of 10% ferric ammonium sulfate solution.

[0038] Implementation method of hydrogen sulfide test colorimetric test tube: see Figure 1, in which 1 is a vacuum glass tube with equal cross-section, 2 is a capillary section, and 3 is a hydrogen sulfide test test solution. h 1 is the height of the test liquid, H 2 For the empty height of the equal-section vacuum tube, the outer diameter of the medium-section vacuum glass tube in this embodiment is 8 mm, the total height is 11.5 cm, and the height H of the test liquid in the glass tube is 1 is 1 cm, and the height of the space is H 2 is 8 cm, and the capillary segment length is 2.5 cm.

[0039] The formula of the hydrogen sulfide test test solution in the test tube of this embodiment is: composed of 1.5 parts of 2%...

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Abstract

An experiment test solution used fro quickly determining hepatic gas is prepared from 1-2 volume of 2% para-azyl methyl toluidine salt, 10-25 volume of 6% sulfuric acid solution and 4-6 volume of 10% molysite solution. The experiment test tube of hepatic gas is also disclosed.

Description

[0001] One. Technical field: the present invention relates to a kind of microbial biochemical testing method and testing equipment thereof, particularly relate to a kind of rapid test hydrogen sulfide test test liquid and colorimetric test tube thereof. 2. Background technology: [0002] The hydrogen sulfide test is one of the commonly used microbial biochemical test methods. Its principle is: some bacteria can reduce sodium thiosulfate, sodium sulfate, and sodium sulfite in the nutrient solution to generate hydrogen sulfide. The negative or positive reaction of hydrogen sulfide can be used as a test for different The basis for the identification of bacteria. For example: Salmonella, Citrobacter freundii, Edwardsiella lentica, Lecleria and Proteus are positive reactions, and other genera are negative reactions. [0003] At present, in microbial biochemical tests, the traditional test method of hydrogen sulfide test is to add ferrous sulfate or ferric ammonium citrate to the cu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/03
Inventor 刘五一崔纪周白莉武拥军刘凤祥
Owner 白莉
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