Nanometer silver antibiotic powder fixed by silk fibroin and preparation method thereof

A technology of nano-silver and antibacterial powder, which is applied in the fields of botanical equipment and methods, animal repellents, biocides, etc., can solve the problem of difficult to realize the preparation of silk fibroin-immobilized nano-silver antibacterial powder, the concentration of nano-silver fibroin sol Low cost, without considering the problems of nano-silver antibacterial powder, etc., to achieve the effects of good biocompatibility, simple manufacturing process and low cost

Inactive Publication Date: 2007-10-03
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yong Zhou, Chen Wenxing and others published a kind of in situ reduction of silk fibroin protein to prepare nano-precious metal wire in Chem.Commun. Su sol method, but did not consider the use of silk fibroin as carrier immobilized nano-silver antibacterial powder preparation
Moreover, the nano-silver fibroin sol obtained by this method has a low concentration, is not easy to dry and solidify, and is difficult to realize the preparation of silk fibroin immobilized nano-silver antibacterial powder

Method used

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  • Nanometer silver antibiotic powder fixed by silk fibroin and preparation method thereof
  • Nanometer silver antibiotic powder fixed by silk fibroin and preparation method thereof
  • Nanometer silver antibiotic powder fixed by silk fibroin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Select clean and decontaminated silk in 0.5% Na 2 CO 3 In aqueous solution, degumming twice at 100°C, 1 hour each time, bath ratio 1:50. The degummed silk fibroin was dried in a drying oven at 80°C until dry.

[0024] 2. Place the dried silk fibroin in CaCl 2 : C 2 h 5 OH:H 2 In the mixed solution of O=1:2:8 (molar ratio), bath ratio 1:10, dissolve at 80±5°C for 30 minutes, filter the solution under reduced pressure, put it in a cellulose semi-permeable membrane, and dialyze it with distilled water for desalination. Make silk fibroin solution, its concentration is 0.5~15%, preferably concentrate or dilute to about 10% silk fibroin solution.

[0025] 3. Take 10g of the above silk fibroin solution and add it dropwise to 10g of 0.1% AgNO 3 In the aqueous solution, use 20% NaOH solution to adjust the pH value of the mixed solution to 9-10, place it at 25°C, the color of the solution gradually changes from colorless to yellow, and react for 10 hours to prepare the ...

Embodiment 2

[0031] 1. Silk degumming is the same as in Example 1.

[0032] 2. Place the dried silk fibroin in 40% CaCl 2In the solution, the bath ratio is 1:20, dissolved at 100°C for 5 minutes, filtered the solution under reduced pressure, installed in a cellulose semipermeable membrane, dialyzed and desalted with distilled water, and made silk fibroin solution with a concentration of 0.5% to 15%. It is best to concentrate or dilute to about 8% silk fibroin solution.

[0033] 3. Take 10g of the above silk fibroin solution and add it dropwise to 10g of 0.2% AgNO 3 In the aqueous solution, use 20% NaOH solution to adjust the pH value of the mixed solution to 8-9, place it at 10°C, the color of the solution gradually changes from colorless to yellow, and place it for 12 hours to obtain the nano-silver fibroin solution.

[0034] 4. Spray-dry the prepared nano-silver fibroin solution at an inlet temperature of 185-190°C and an outlet temperature of 80-85°C to obtain silk fibroin with an ave...

Embodiment 3

[0036] 1. Silk degumming is the same as in Example 1.

[0037] 2. Put the dried silk fibroin in 6mol / L hydrochloric acid solution, bath ratio 1:25, dissolve at 85°C for 1.5 hours, filter the solution under reduced pressure, put it in a cellulose semi-permeable membrane, and dialyze it with distilled water for desalination. Make silk fibroin solution, its concentration is 0.5~15%, preferably concentrate or dilute to about 8.5% silk fibroin solution.

[0038] 3. Take 10g of the above silk fibroin solution and add it dropwise to 10g of 0.15% AgNO 3 In the aqueous solution, use 10% HCl solution to adjust the pH value of the mixed solution to 4-5, place it at 5°C, the color of the solution gradually changes from colorless to yellow, and place it for 24 hours to obtain the nano-silver fibroin solution.

[0039] 4. Vacuum freeze-dry the prepared nano-silver fibroin solution at -50°C to obtain uniform particles with an average particle size of silk fibroin of about 175nm and an avera...

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Abstract

An antibacterial silk protein carried nano-Ag powder used for cosmetics, health-care food, enzyme immobilizing materials, biosensor, artificial skin or muscle, etc is prepared proportionally from silk protein and Ag nanoparticles. Its preparing process is also disclosed.

Description

(1) Technical field [0001] The invention relates to a natural polymer antibacterial powder, in particular to a composite antibacterial powder made of silk fibroin and nano-silver and a preparation method of the composite antibacterial powder. (2) Background technology [0002] With the development of the economy and the improvement of people's living standards, the health of various daily necessities has become the trend of today's social development. In recent years, it has become a trend to add antibacterial materials to various daily products. At present, the most widely used antibacterial materials are silver series products. Silver series antibacterial materials are a kind of inorganic antibacterial materials with the characteristics of persistence, broad spectrum, good heat resistance, high safety, and not easy to produce drug resistance. However, the antibacterial effect of ordinary silver preparations is weak, and the processing of metallic silver into nano-silver c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/10A01N25/12A01N59/16
Inventor 艾仕云曲祥金周杰李金焕时伟杰马治军李晓晨
Owner SHANDONG AGRICULTURAL UNIVERSITY
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