Heavy producing bacterial strain F64 for Wuyi bacterin, and fermentation process

A technology of wuyin and strains, applied in the field of new microorganism strains, can solve the problems affecting the industrialized production of wuyin, energy consumption, increase in cultivation time, etc., and achieve the effect of promoting sustainable agricultural development, expanding the scope of use, and preventing diseases. improved effect

Inactive Publication Date: 2007-10-10
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

In recent years, with the increase of production strains, the strains have degenerated seriously, and the current potency is only 1800-2000ug/ml, and the cultivation time has also inc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Medium formula

[0016] Formula 1 Corn flour 20mg / ml, soybean meal powder 32mg / ml, glucose 27.2mg / ml, ammonium chloride 3mg / ml, calcium carbonate 3mg / ml, magnesium sulfate 0.2mg / ml, ferrous sulfate 0.2mg / ml, chlorine Sodium chloride 0.2mg / ml, dipotassium hydrogen phosphate 0.2mg / ml;

[0017] Formula 2 Corn flour 15mg / ml, soybean meal powder 35mg / ml, glucose 35mg / ml, ammonium chloride 1mg / ml, calcium carbonate 1mg / ml, magnesium sulfate 0.5mg / ml, ferrous sulfate 0.5mg / ml, chloride Sodium 0.1mg / ml, dipotassium hydrogen phosphate 0.5mg / ml;

[0018] Formula 3 corn flour 25mg / ml, soybean cake powder 20mg / ml, glucose 20mg / ml, ammonium chloride 5mg / ml, calcium carbonate 5mg / ml, magnesium sulfate 0.1mg / ml, ferrous sulfate 0.1mg / ml, chloride Sodium 0.5mg / ml, dipotassium hydrogen phosphate 0.1mg / ml.

Embodiment 2

[0019] Embodiment 2 shaker fermentation production method

[0020] Containing corn flour 20mg / ml, soybean cake powder 32mg / ml, glucose 27.2mg / ml, ammonium chloride 3mg / ml, calcium carbonate 3mg / ml, magnesium sulfate 0.2mg / ml, ferrous sulfate 0.2mg / ml, chlorine F64 was inoculated in a culture medium with sodium chloride 0.2 mg / ml and dipotassium hydrogen phosphate 0.2 mg / ml, and cultured on a shaker at 28° C. with a rotation speed of 180 rpm / min for 72 hours. After the fermentation broth is filtered, the mycelium is removed, and the titer is determined to reach 6500u / ml by a biological titer detection method. The concentration of 2 times the potency can reach or exceed the product quality standard (10000u / ml) of Wuyiencin with 1% active ingredient, and the concentrated solution can be directly used as a sterilizer.

Embodiment 3

[0021] Embodiment 3 fermentation tank production method

[0022] After the F64 bacterial strain is activated, it is cultivated by primary seeds for 32 hours, transferred to the fermentation medium according to the inoculation amount of 10% (v / v), and expanded and cultivated in the fermenter for 40-50 hours.

[0023] The fermentation medium is: corn flour 20mg / ml, soybean cake powder 32mg / ml, glucose 27.2mg / ml, ammonium chloride 3mg / ml, calcium carbonate 3mg / ml, magnesium sulfate 0.2mg / ml, ferrous sulfate 0.2mg / ml ml, sodium chloride 0.2mg / ml, dipotassium hydrogen phosphate 0.2mg / ml.

[0024] The fermentation conditions are: temperature 30° C., rotation speed 200 rpm, ventilation rate 1:0.9, tank pressure 0.10 MPa. Ferment for 78 hours. After the fermentation broth was filtered, the mycelium was removed, and the titer was determined by the tube-and-disc method.

[0025] Results: The potency reaches 6500u / ml, and the concentrated solution with a potency of 10000u / ml can be us...

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Abstract

This invention provides Streptomyces anhygroscopicus var. wuyiensis F64 (CGMCC No.1967), which can be used to prepare a novel biological pesticide, wuyiencin with the fermentation solution titer higher than 5000 U/mL, in conventional culture conditions. This invention also optimizes the culture method and the culture medium formula, by which the fermentation solution titer can reach 6000-6500 U/mL. This invention can shorten the fermentation time by 5-10 h, increase the fermentation solution titer by 150-200%, increase the yield by 100% and improve the disease-preventative effect by 15-20%.

Description

technical field [0001] The present invention relates to a new microbial strain, specifically a high-yield strain (Streptomyces anhygroscopicus var. wuyiensis) F64 of Streptomyces anhygroscopicus var. . Background technique [0002] Biopesticides are a new direction for the development of the pesticide industry in the 21st century, and antibiotic fungicides are one of the fastest-growing products in the industrialization of biopesticides. Wuyiencin is a biopesticide product developed by the Chinese Academy of Agricultural Sciences that has the effect of preventing disease and increasing production. It is derived from an antibiotic produced by Streptomyces anhygroscopicus var. wuyiensis (Acta Microbiology, 24: 401-402, 1984), which is mainly used to prevent and control fungal diseases of crops. It has the characteristics of high efficiency, broad spectrum, low toxicity and environmental safety. Therefore, it has broad application prospects in ecological agriculture and green ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02C12P1/04C12R1/465
Inventor 张克诚石义萍付月灵王立东阿拉坦夫王万群展丽然
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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