Process for the detecting of hydrogen peroxide

A technology of hydrogen peroxide and detection method, applied in the direction of electrochemical variables of materials, preparation of samples for testing, etc., can solve the problems of difficult long-term storage, decreased stability, decreased activity, etc., and achieves effective and wide-ranging electrochemical effects of products. Detection limit, rapid response effect

Inactive Publication Date: 2007-10-17
NANJING NORMAL UNIVERSITY
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hydrogen peroxide is generally detected by horseradish peroxidase (HRP) catalyzing the reaction of hydrogen peroxide on the electrode to generate a current response. An electron mediator is us

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for the detecting of hydrogen peroxide
  • Process for the detecting of hydrogen peroxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the detection method of hydrogen peroxide,

[0028] (1), preparation of nano-scale ferrous sulfide: (NH4) 2 SO 4 ·FeSO 4 ·6H 2 O (0.39121g, 0.04M) adds 25mL nitrogen deoxygenation secondary water, after dissolving, add dodecylamine hydrochloride (0.0776g, 0.014M) and dissolve completely to obtain solution A; Na 2 S 9H 2 O (0.1201g, 0.04M), add 10mL nitrogen deoxygenation secondary water to dissolve, then dissolve dodecylamine hydrochloride (0.014M) in 15mL nitrogen deoxygenation secondary water, and then mix Na 2 S and dodecylamine hydrochloride solution, to obtain milky suspension B. Under ultrasonication, add solutions A and B successively into a three-necked flask protected by a nitrogen atmosphere, the reaction occurs immediately, the solution turns black, continue ultrasonication for 10 minutes and then centrifuge, successively use deoxygenated secondary water, nitrogen-passed absolute ethanol, and wash three times each Precipitation, during the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method of detecting hydrogen peroxide, which includes steps: (1) preparation of nano ferrous sulfide: dissolving ammonium ferrous sulfate into secondary water after removing oxygen by blowing nitrogen, adding mixing solution of sulfide and surfactant into the water solution and mixing at ultrasound condition, after reaction, centrifuge washing, depositing, vacuum drying, and obtaining the nano ferrous sulfide sample; wherein mole ration of the sulfide and the surfactant is 1:1, dosing of the surfactant is 0.014N; (2) checking preparation: dropping and coating suspension and polymer film of the ferrous sulfide nano particles on glass-carbon electrode in turn, airing, and aging; (3) detection of hydrogen peroxide: continuously adding hydrogen peroxide into electrolytic cell to detect the hydrogen peroxide, recording ampere response of the hydrogen peroxide, and obtaining detection result. The invention uses nano ferrous sulfide to replace HRP for detecting hydrogen peroxide, due to nano material having large specific surface area, the method increases sensitivity of detection and is simple, convenient, fast, sensitive and stable.

Description

technical field [0001] The invention relates to a novel, simple, fast, sensitive and stable hydrogen peroxide detection method. Background technique [0002] Hydrogen peroxide is a very important active intermediate in clinical medicine and environmental chemistry. The detection of its concentration has important research significance in the fields of chemical analysis, biological analysis, medicine, clinical diagnosis, and environmental analysis. At present, the methods for the determination of hydrogen peroxide mainly include polarography, flow injection conductometric method, electroanalysis, fluorescence analysis, spectrophotometry and chemiluminescence. Among them, the electroanalysis method is widely used because of its simplicity, rapidity, sensitivity and low price. Hydrogen peroxide is generally detected by horseradish peroxidase (HRP) catalyzing the reaction of hydrogen peroxide on the electrode to generate a current response. An electron mediator is used between...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N27/42G01N1/28
Inventor 戴志晖包建春倪嘉
Owner NANJING NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap