Process for the detecting of hydrogen peroxide

Abstract

The invention discloses a method of detecting hydrogen peroxide, which includes steps: (1) preparation of nano ferrous sulfide: dissolving ammonium ferrous sulfate into secondary water after removing oxygen by blowing nitrogen, adding mixing solution of sulfide and surfactant into the water solution and mixing at ultrasound condition, after reaction, centrifuge washing, depositing, vacuum drying, and obtaining the nano ferrous sulfide sample; wherein mole ration of the sulfide and the surfactant is 1:1, dosing of the surfactant is 0.014N; (2) checking preparation: dropping and coating suspension and polymer film of the ferrous sulfide nano particles on glass-carbon electrode in turn, airing, and aging; (3) detection of hydrogen peroxide: continuously adding hydrogen peroxide into electrolytic cell to detect the hydrogen peroxide, recording ampere response of the hydrogen peroxide, and obtaining detection result. The invention uses nano ferrous sulfide to replace HRP for detecting hydrogen peroxide, due to nano material having large specific surface area, the method increases sensitivity of detection and is simple, convenient, fast, sensitive and stable.

Description

technical field [0001] The invention relates to a novel, simple, fast, sensitive and stable hydrogen peroxide detection method. Background technique [0002] Hydrogen peroxide is a very important active intermediate in clinical medicine and environmental chemistry. The detection of its concentration has important research significance in the fields of chemical analysis, biological analysis, medicine, clinical diagnosis, and environmental analysis. At present, the methods for the determination of hydrogen peroxide mainly include polarography, flow injection conductometric method, electroanalysis, fluorescence analysis, spectrophotometry and chemiluminescence. Among them, the electroanalysis method is widely used because of its simplicity, rapidity, sensitivity and low price. Hydrogen peroxide is generally detected by horseradish peroxidase (HRP) catalyzing the reaction of hydrogen peroxide on the electrode to generate a current response. An electron mediator is used between...

AI Technical Summary

Problems solved by technology

Hydrogen peroxide is generally detected by horseradish peroxidase (HRP) catalyzing the reaction of hydrogen peroxide on the electrode to generate a current response. An electron mediator is used between HRP and the electrode to transfer electrons, and HRP is not only expensive, not easy to store for a long time, but also easy to inactivate, resulting in reduced activity and stability

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