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Total alkaloid of lotus leaves extraction and its preparation method

A technology of lotus leaf total alkaloids and total alkaloids, applied in the field of medicine, can solve problems such as unseen preparation methods and uses

Inactive Publication Date: 2007-12-12
石任兵 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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So far, there are no patents related to lotus leaf total alkaloid extract and its preparation method and use

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  • Total alkaloid of lotus leaves extraction and its preparation method
  • Total alkaloid of lotus leaves extraction and its preparation method
  • Total alkaloid of lotus leaves extraction and its preparation method

Examples

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Embodiment 1

[0050] Embodiment 1: preparation process of lotus leaf total alkaloid extract

[0051] Take 1 kg of lotus leaf decoction pieces, add 16 L of 90% ethanol, reflux extraction 3 times, extract 1.5 hours each time, recover solvent under reduced pressure, add 1% hydrochloric acid aqueous solution to disperse and dissolve the extract, so that the acid aqueous solution concentration is 0.165 mg / mL (in total alkaloid meter), centrifuged at a speed of 3000 rev / min for 30min, got the supernatant, passed through 300mL D001-CC macroporous strongly acidic cation exchange resin, the adsorption flow rate was 10BV / h, and the diameter-to-height ratio of the resin column was 1: 8. The loading amount is 27.5mg / mL (based on the total alkaloid content), and 50% ethanol is eluted with 5 times the volume of the resin for impurity removal. %) to elute 7 times the resin volume, the elution flow rate is 10BV / h, collect the 70% ammonia ethanol eluate, recover the solvent, and dry under reduced pressure, ...

Embodiment 2

[0053] Embodiment 2: preparation process of lotus leaf total alkaloid extract

[0054] Take 1 kg of lotus leaf decoction pieces, add 20 L of 70% ethanol, reflux extraction 3 times, extract for 1.5 hours each time, recover the solvent under reduced pressure, add 0.5% hydrochloric acid aqueous solution to the extract to disperse and dissolve, so that the concentration of the acid aqueous solution is 0.33 mg / mL (based on the total alkaloid meter), centrifuged at a speed of 3000 rev / min for 30min, got the supernatant, passed through 300mL D001-CC macroporous strongly acidic cation exchange resin, the adsorption flow rate was 5BV / h, and the resin column diameter-to-height ratio was 1: 10. The sample loading is 30.25mg / mL (based on the total alkaloid content), and the water is eluted to 10 times the volume of the resin for impurity removal. Elute 5 times the volume of the resin, the elution flow rate is 10BV / h, collect the 90% ammonia ethanol eluate, recover the solvent, dry under r...

Embodiment 3

[0056] Embodiment 3: preparation process of lotus leaf total alkaloid extract

[0057] Take 1 kg of lotus leaf decoction pieces, add 25 L of 50% ethanol, reflux extraction twice, extract for 2 hours each time, recover solvent under reduced pressure, add 1.5% hydrochloric acid aqueous solution to disperse and dissolve the extract, so that the acid aqueous solution concentration is 0.495 mg / mL (in total alkaloid meter), centrifuged at a speed of 3000 rev / min for 30min, got the supernatant, passed through 300mL D001-CC macroporous strongly acidic cation exchange resin, the adsorption flow rate was 3BV / h, and the resin column diameter-to-height ratio was 1: 6. The loading amount is 24.75mg / mL (based on the total alkaloid content), and 10% ethanol is used to elute 6 times the volume of the resin for impurity removal. %) to elute 7 times the resin volume, the elution flow rate is 10BV / h, collect the 50% ethanol eluate, recover the solvent, dry under reduced pressure, which is the lo...

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Abstract

The invention discloses total alkaloid extract extracted from lotus leaf and its preparation method. The extract mainly comprises nuciferine, N-demethylnuciferine, O-demethyl nuciferine, oxopurpureine, nuciferine, tulipiferine, N-demethyl armepavine, 2-hydroxy-1-methoxy aporphine, armepavine, N-methyl isococlaurine, N-methyl coclaurine, dehydronuciferine, dehydronuciferine and its derivant, etc. The extractcan be extracted through one or more of methods of solvent-extraction, solvent extraction, ion exchange resin process, macroporous adsorbent resins method, extraction by supercritical fluid, column chromatography, and liquid-liquid countercurrent distribution chromatography. The total amount of different kinds of alkaloids in the extract is 5-100% (w / w), wherein nuciferine content occupies 5-100% (w / w).

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a total alkaloid extract of traditional Chinese medicine lotus leaf, a preparation method, a quality control method and its application in the fields of medicine and health food. Background technique [0002] The lotus leaf is the dry leaf of Nelumbo nucifera Gaertn. Traditional medicine believes that the lotus leaf is bitter and cold in nature and has the activities of clearing heat and dampness, promoting hair growth and clearing yang, clearing heart and removing heat, and stopping bleeding and diuresis. According to the "Compendium of Materia Medica" it is recorded that "taking lotus leaves makes people thin and weak", "generates vitality, helps the spleen and stomach, astringent essence and turbidity, dissipates congestion, reduces swelling and pain, and causes acne". Modern pharmacological studies have shown that lotus leaf has the functions of reducing fat and...

Claims

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Application Information

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IPC IPC(8): A61K36/62A61P3/04A61P3/06A61P1/16A61P39/06C07D217/20C07D217/24C07D221/06C07D491/04A61K127/00A61K125/00A61K131/00A61K133/00A61K135/00
CPCY02P20/54
Inventor 刘斌石任兵王伟王玉霞王玲玲王东方
Owner 石任兵
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