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Method of liquid membrane separation for extracting alkaloid of Macleaya cordata

A liquid-membrane separation and blow-back technology, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., can solve the problems of cumbersome operation, high consumption of raw materials, complicated production process, etc., and achieve simple operation and extraction fast process results

Inactive Publication Date: 2007-12-26
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These extraction methods have disadvantages such as complex production process, cumbersome operation, low product recovery rate, high energy consumption, and large consumption of raw materials.
Emulsion liquid membrane method is a simple, fast, efficient, highly selective, economical and energy-saving new separation and extraction technology, which has been applied in the fields of environment, chemical production, hydrometallurgy, etc., but has not been applied to the extraction of total alkaloids in Chinese herbal medicines. extraction, and the film solvents of the existing emulsion liquid film are usually toluene, cyclohexane, dichloromethane and kerosene; these solvents have certain toxicity, and are not suitable for the use of Chinese herbal medicines for humans and animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Preparation of Boluohui extract: Weigh 1.0 g of Boluohui powder, add 30 mL of 0.1mol / L HCl, ultrasonically extract for 30 minutes, centrifuge for 10 minutes in a centrifuge with a rotating speed of 4000r / min, take out the filtrate, and pour it into the filter residue Then add 30 mL of 0.1 mol / L HCl, ultrasonically extract for 30 min, then centrifuge for 10 min, and combine the filtrates. Dilute the filtrate to 500 mL with 0.1 mol / L HCl, filter, adjust the pH to 4 with saturated sodium hydroxide, and set aside.

[0016] (2) Preparation of emulsion: take 42mL of film solvent edible oil, 3.5mL of surfactant total amount, wherein Span 80 is 0.5mL, n-decyl alcohol is 3mL, phospholipid carrier 4.5mL, the three are mixed, each Take 20 mL of this mixture, add 20 mL of 0.1 mol / L HCl solution at a volume ratio of 1:1, and stir at 8000 rpm for 1 min to obtain a stable W / O emulsion.

[0017] (3) Separation and extraction of alkaloids: Add the hydrochloric acid extraction solut...

Embodiment 2

[0028] (1) Preparation of Boluohui extract: Weigh 2 kg of Boluohui extract powder, add 120 L of 0.1mol / L HCl, ultrasonically extract for 60 minutes, centrifuge for 10 minutes in a centrifuge with a rotating speed of 4000r / min, take out the filtrate, Add 120 L of 0.1 mol / L HCl to the filter residue, ultrasonically extract for 60 min, then centrifuge for 10 min, and combine the filtrates. Dilute the filtrate to 2400L with 0.1mol / L HCl, filter, adjust pH=4 with saturated sodium hydroxide, and set aside;

[0029] (2) Preparation of emulsion: get film solvent edible oil 124L, surfactant total amount is 36L, wherein Span80 is 14L, n-decyl alcohol is 22L, phospholipid carrier 40L, three are mixed, then this mixture is mixed with Add 0.7 mol / L HCl solution into the milk maker at a volume ratio of 4:1, and stir for 15 minutes at a speed of 10,000 rpm to obtain a stable W / O emulsion.

[0030] (3) Separation and extraction of alkaloids: Add the hydrochloric acid extraction solution and ...

Embodiment 3

[0041] (1) Preparation of Boluohui extract: Weigh 1.0 g of Boluohui powder, add 50 mL of 0.1mol / L HCl, ultrasonically extract for 50 minutes, centrifuge for 10 minutes in a centrifuge with a rotating speed of 4000r / min, take out the filtrate, and pour it into the filter residue Then add 50 mL of 0.1 mol / L HCl, ultrasonically extract for 50 min, then centrifuge for 10 min, and combine the filtrates. Dilute the filtrate to 900 mL with 0.1 mol / L HCl, filter, adjust the pH to 4 with saturated sodium hydroxide, and set aside.

[0042] (2) Preparation of emulsion: Take 37mL of film solvent edible oil, 7mL of surfactant total amount, wherein Span 80 is 1mL, n-decyl alcohol is 6mL, phospholipid carrier 6mL, the three are mixed, each take this mixture Add 10 mL of 0.1 mol / L HCl solution to 20 mL at a volume ratio of 2:1, and stir for 2 min at a speed of 9000 rpm to obtain a stable W / O emulsion.

[0043] (3) Separation and extraction of alkaloids: add the hydrochloric acid extraction s...

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PUM

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Abstract

The present invention discloses a method for extracting total alkaloid of macleaya cordata by using liquid-membrane separation process. The preparation of its emulsion is characterized by that it includes the following steps: using edible oil as membrane solvent, using mixture of Span 80 and assistant of surfactant or Span 80 as surfactant, using organic phosphate as carrier, making macleaya cordata hydrochloric acid extrat or sulfuric acid extract whose pH is 4 and the emulsion be added into an extractor according to the emulsion-water volume ratio of 50:5-50:20, making extraction for 5-15 min under the condition of low-stirring speed of 200 rpm-300 rpm, heating by means of water bath and emulsion-breaking, then separating out oil layer so as to obtain water phase, said water phase is solution of total alkaloid of macleaya cordata.

Description

technical field [0001] The invention relates to a method for liquid membrane separation and extraction of alkaloids in Boluohui, which can be applied to the extraction and production of medicinal materials in pharmaceutical factories, or to test their active ingredients in analytical chemistry. Background technique [0002] The main components of the Chinese herbal medicine Boluohui extract are sanguinarine, chelerythrine and alkaloids such as protopine and allocryptine. At present, these alkaloids have been developed into medicines, biological pesticides, veterinary drugs and other products at home and abroad. However, the current Boluohui alkaloid extraction process is mainly liquid-liquid extraction. For example, Mao Peng, Zhou Le, Ran Xiaoya, Yuan Chao, and Wang Yongxue published the "Preliminary Study on the Extraction Process of Boluohui Alkaloids" in Northwest Agricultural Journal 2004, Issue 02. The alkaloids that fall back into the whole plant are subjected to ult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/66
Inventor 马铭梁锋陈波姚守拙
Owner HUNAN NORMAL UNIVERSITY
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