Method for preparing cadmium selenide quantum dots by using microbial cells

A technology of microbial cells and quantum dots, applied in the direction of bacteria, fermentation, etc., can solve the problems affecting the uniformity and harshness of the product, and achieve the effect of low synthesis cost, simple method and good uniformity

A technology of microbial cells and quantum dots, applied in the direction of bacteria, fermentation, etc., can solve the problems affecting the uniformity and harshness of the product, and achieve the effect of low synthesis cost, simple method and good uniformity

CN101096687AInactive Publication Date: 2008-01-02WUHAN UNIV
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  • Method for preparing cadmium selenide quantum dots by using microbial cells
  • Method for preparing cadmium selenide quantum dots by using microbial cells
  • Method for preparing cadmium selenide quantum dots by using microbial cells

Examples

Experimental program
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Effect test

Embodiment 1

[0059] 1. Culture of Yeast Cells

[0060] First, inoculate a single colony of Saccharomyces cerevisiae into YPD medium, culture with shaking at 30°C for 24 hours; set aside.

[0061] 2. Selenization treatment of Saccharomyces cerevisiae cells

[0062] 0.05 milliliters, 0.5 milliliters, and 5 milliliters of Na2SeO3 (100 mg / liter) solutions were respectively added to three bottles of Saccharomyces cerevisiae cell suspension obtained in step 1 (50 milliliters of cell suspension in each bottle), and cultured with shaking at 30°C for 0-25 Hour.

[0063] 3. The synthesis of cadmium treatment of cells and the synthesis of CdSe quantum dots in cells

[0064] The cell suspension obtained after step 2 was centrifuged at 8000 rpm for 3 minutes, and the supernatant was discarded. and resuspended in fresh YPD medium. Then add 0.5 ml of 100 mmol / L CdCl2 solution, and shake at 30° C. for 12 hours.

[0065] 4. Extraction of intracellular CdSe quantum dots

[0066] The cell suspension ob...

Embodiment 2

[0070] 1. Yeast cell culture

[0071] First, inoculate a single colony of Saccharomyces cerevisiae into YPD medium, culture with shaking at 30°C for 24 hours; set aside.

[0072] 2. Selenization treatment of Saccharomyces cerevisiae cells

[0073] 0.5 ml of Na2Se03 (100 mg / L) solution was added to 50 ml of Saccharomyces cerevisiae cell suspension obtained in step 1, and cultured with shaking at 30° C. for 24 hours.

[0074] 3. The synthesis of cadmium treatment of cells and the synthesis of CdSe quantum dots in cells

[0075] The cell suspension obtained after step 2 was centrifuged at 8000 rpm for 3 minutes, and the supernatant was discarded. and resuspended in fresh YPD medium. Then add 0.05 milliliters, 0.5 milliliters and 5 milliliters of CdCl2 solution (concentration is 100 mmol / liter) respectively, and shake at 30 DEG C for 0-40 hours.

[0076] 4. Extraction of intracellular CdSe quantum dots

[0077]The cell suspension obtained in step 3 was centrifuged at 8000 rpm...

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Abstract

The invention discloses a synthesizing method of cadmium selenide quantum point through microbe cell, which comprises the following steps: A. A. preparing microbe cell; B. selenidizing the microbe cell; C. cadmating the cell to culture continuously; D. extracting CdSe quantum point from cell; E. purifying the CdSe quantum point. The invention simplifies the operation without organic metal compound and inert gas to protect, which is beneficial to do industrialization synthesis of nanometer particle (quantum point).

Description

technical field [0001] The invention belongs to the fields of nanoscience, microbiology and material synthesis, and in particular relates to a method for preparing cadmium selenide (CdSe) quantum dots by utilizing microbial cells. Background technique [0002] Quantum dots (Quantum Dots, QDs) generally refer to semiconductor nanocrystals with a radius smaller than or close to the exciton Bohr radius. They have unique fluorescent nano-effects, such as wide excitation spectrum and continuous distribution, narrow and symmetrical emission spectrum, and luminescence wavelength can be adjusted by changing particle size and composition, strong fluorescence intensity, slow bleaching rate, and high sensitivity, so nearly ten It has aroused people's widespread attention in recent years, and has broad application prospects in luminescent materials, photocatalysis, photosensitive sensors, and fluorescent probe labeling. In particular, quantum dots composed of group IIB and VIA elements...

Claims

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Application Information

Patent Timeline
02 Jan 2008
Publication
CN101096687A
IPC
C12P3/00; C12N1/20
Inventors
刘茴茴; 崔然