Method for preparing gamma-decalactone and improving output by biotransformation and separated coupling

A technology for decanolactone and products, which is applied in the field of biological preparation of aromatic compounds, can solve the problems of complex transformation process, metabolic pathway and regulation mechanism, etc. The effect of conversion efficiency

Inactive Publication Date: 2008-01-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also many reports on the production of γ-decalactone by conversion of non-hydroxy fatty acids, but the metabolic pathway and regulato...

Method used

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  • Method for preparing gamma-decalactone and improving output by biotransformation and separated coupling
  • Method for preparing gamma-decalactone and improving output by biotransformation and separated coupling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 The effect of different addition methods of castor oil on the conversion of γ-decalactone.

[0026] Two different preparation methods were compared by growing cell culture method and direct fermentation with castor oil as carbon source. Growth cell transformation, that is, when the bacteria grow to the equilibrium period and the glucose is basically exhausted, castor oil is directly added to allow the microorganisms to undergo biotransformation while multiplying and growing. The growth process was used for transformation, and the yield of decanolide was significantly higher after adding castor oil for 48 hours. When castor oil is used as carbon source for direct fermentation, the product can also be prepared, but the yield is low. The result is shown in Figure 1.

Embodiment 2

[0027] Example 2 Effects of different resin additions on the conversion yield of γ-decalactone.

[0028] Add resin AB-8, the addition amount (g / L) is 25, 50, 75, 100, 125 respectively, the conversion situation of γ-decalactone is shown in Figure 2. With the increase of the amount of resin, the adsorption amount of γ-decalactone gradually increased, and the overall concentration was the highest when the amount of resin added was 75, reaching 1.73g / L. Continue to increase the amount of resin, but the overall yield did not increase significantly.

Embodiment 3

[0029] Example 3 The effect of different castor oil dosages on the conversion of γ-decalactone.

[0030] The amount of resin is constant, after conversion by adding different amounts of castor oil, the content of γ-decalactone is measured, and the results are shown in Figure 3. As can be seen from Figure 3, the decanolactone output is higher when the substrate concentration is 25g / L. There is an optimum value for the amount of substrate added, and then with the increase of substrate concentration, the yield will decrease.

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Abstract

The invention discloses a method for preparing gamma-decyl inner ester and biological transforming, separating and coupling and improving the yield in the technique field of preparing the aromatic compound with biological method. The invention provides a strain Yarrowia sp. CGMCC 2.1405 with the high-effective transformation ability, which improves the yield of gamma--decyl inner ester and achieves the separation of the product with the transformation, separation and coupling technique by culturing the cell, preparing the total cell multi-enzyme system, biological transformation of the total cell multi-enzyme system, adding the macroreticular adsorbing resin in the product transformation process. The invention reduces the product influence for the bacterial, which improves the yield, and achieves the effective original position separation of the product.

Description

technical field [0001] The invention relates to a method for preparing gamma-decalactone and coupling biotransformation and separation to increase yield, belonging to the technical field of biological preparation of aromatic compounds. The present invention relates to a novel process for the preparation of gamma-decalactone. Background technique [0002] γ-decalactone (gamma decalactone), also known as gamma decalactone, is a five-membered ring lactone compound. γ-Decalactone has become an important aromatic compound because of its organoleptic properties. It has a fruity, peach-like aroma and aroma, with a typical coconut aroma, and the aroma is lighter than peach aldehyde. It is known for its attractive The characteristics of peach flavor and low aroma threshold are widely used in the fragrance industry, mainly for the preparation of cream, peach, citrus and coconut flavors. In general, γ-decalactone can be produced from fruit. However, its abundance in the fruit is so ...

Claims

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Application Information

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IPC IPC(8): C12P17/04C12R1/645
Inventor 徐岩王栋于伟
Owner JIANGNAN UNIV
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