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Method for extracting stevioside

A technology of stevioside and extraction method, which is applied in the field of plant extraction and clean production, can solve the problems of a large amount of waste water, short service life of resin, low product purity, etc., and achieve the effects of reduced waste water discharge, increased service life and high purity

Active Publication Date: 2008-02-06
NANJING JIUSI HIGH TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to propose a method for extracting steviol glycosides by using an integrated membrane separation process in order to solve the problems of low purity of products obtained by conventional methods, short service life of resins, and large amounts of waste water.

Method used

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  • Method for extracting stevioside

Examples

Experimental program
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Effect test

Embodiment 1

[0044] As shown in accompanying drawing 1, take by weighing 50Kg stevia leaves, soak in 1m 3 Soak in a container of water for 4 hours at room temperature, then pass through a coarse sieve to trap large leaves, and collect the permeate to obtain stevioside extract.

[0045] Add CaO to the stevioside extract to adjust pH=8, add 10Kg of FeCl 3 , then stirred at a speed of 100 rev / min for 10 min, and settled for 1 h, so that impurities such as a part of colloids and pigments were flocculated and precipitated, and the supernatant entered membrane separation pre-filtration equipment (manufactured by Nanjing Jiusi High-Tech Co., Ltd., with a membrane area of ​​0.1 m 2 , the following examples are the same) the supernatant is filtered by a tubular titanium metal membrane with a pore size of 200nm, and the operation mode of cross-flow filtration is adopted, the operating pressure is 0.15Mpa, and the cross-flow velocity is 1m / s. The permeate obtained through membrane separation and fil...

Embodiment 2

[0051] The extract was prepared according to the method of Example 1.

[0052] The extraction liquid is cross-flow filtered by using a tubular 19-channel zirconia ceramic membrane with a pore size of 20nm, the operating pressure is 0.75Mpa, and the cross-flow velocity is 5m / s. The permeate obtained through membrane separation and filtration enters the first-stage membrane filtration process, and the concentrated solution is added with 1 times of water for top washing, top washing three times, and then the plate and frame filter press presses the residue to make fertilizer and transport it abroad.

[0053] The primary membrane filtration process adopts roll-type polyethersulfone ultrafiltration membrane with a cut molecular weight of 1500 for cross-flow filtration, the operating pressure is 1.2Mpa, the cross-flow velocity is 0.5m / s, the concentration is 15 times, and the permeate is reused as the previous process The extraction water is used, and the concentrated liquid is proc...

Embodiment 3

[0058] Referring to the method of Example 1, the extract of stevioside was prepared.

[0059] Add NaOH to the extract to adjust the pH to 8.5, add 10Kg of polyacrylamide flocculant, then stir at 100 rpm for 15 min, and settle for 0.5 h to allow some impurities such as colloids and pigments to flocculate and precipitate, and the supernatant For membrane separation and filtration, the supernatant is cross-flow filtered by a tubular stainless steel ceramic composite membrane with a pore size of 1 μm. The operating pressure is 0.02Mpa and the cross-flow velocity is 1m / s. The permeate obtained through membrane separation and filtration enters the first-stage membrane filtration process, and the concentrated solution is added with 1 times of water for top washing, top washing three times, and then the plate and frame filter press presses the residue to make fertilizer and transport it abroad.

[0060] The first-stage membrane filtration process uses a hollow fiber polysulfone ultraf...

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Abstract

The present invention relates to an extraction method of stevioside. The invention comprises the following procedures: extracts of stevioside eliminates colloid, pigment, solid suspended substance and fiber through pretreatment and then is made into finished products through processes of first-phase membrane filtration, resin absorption, analytic, desalination, decoloration, second-phase membrane filtration, and spray drying, wherein the penetrant of the first-phase membrane filtration is recycled as the diffusion water, and the penetrant of the second-phase membrane filtration is recycled as desorption solution, while the waste liquid of the resin absorption is discharged or recycled after treatment. The product of the present invention has a high purity, which is greater than 90 percent and superior over the national standard high grade product; the present invention can solve the problems of lower purity product and large amount of wastewater of routine method efficiently, and prolongs the service life of the resin by three times.

Description

technical field [0001] The invention relates to a method for extracting stevioside, which belongs to the fields of plant extraction and clean production. Background technique [0002] Stevia is a new sugar crop. The leaves contain sweet ingredients. According to detection and analysis, there are eight kinds of sweet ingredients in stevia leaves: stevioside (stevloside) (St), steviolbioside (steriolbioside), rebaudioside A (rebaudioside A) ) (R-A), rebaudioside B (rebaudioside B) (R-B), rebaudioside C (rebaudioside C) (R-C), rebaudioside D (rebaudioside D) (R-D), rebaudioside E ( rebaudioside E) (R-E) and dulcoside A (dulcosiole A) (Dul-A), among these glycosides, there are four types of glycosides with high content and economic value: St, R-A, R-C, and D-A. The molecular weight distribution is about 800, and it is collectively called stevioside (alias: stevioside), among which the sweetness of R-A is 350-400 times that of sucrose. Among all stevioside ingredients, it has t...

Claims

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Application Information

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IPC IPC(8): C07H15/24C07H1/08
Inventor 郑书旺范益群霍强张宇郑超仲兆祥郑然
Owner NANJING JIUSI HIGH TECH
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