Ascendant bacterium and application for lead waste water treatment

A technology with dominant bacteria and practicability, applied in the field of environmental bioengineering, can solve the problems of biofilm method limitation, heavy metal poisoning, etc., and achieve the effects of high selectivity, high adsorption, and high tolerance

Inactive Publication Date: 2008-03-05
DALIAN NATIONALITIES UNIVERSITY +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the toxic effect of heavy metals on microorganisms, the application of biofilm method is limited.

Method used

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  • Ascendant bacterium and application for lead waste water treatment
  • Ascendant bacterium and application for lead waste water treatment
  • Ascendant bacterium and application for lead waste water treatment

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: use the method of the present invention to treat the waste water of the water quality purification plant of Dalian Economic and Technological Development Zone. The process is as follows:

[0025] 1. Biofilm cultivation:

[0026] After the activated sludge in the secondary settling tank of a water purification plant in Dalian Economic and Technological Development Zone was cleaned with tap water, a certain amount of cleaned sludge was poured into artificial wastewater, and biofilm was loaded with polyethylene or polypropylene porous rings, and adjusted The water temperature is 10-40°C, intermittent aerobic culture is carried out, and the film-forming time is 5-25 days.

[0027] 2. Domestication, purification and screening of leadophilic dominant strains:

[0028] Acclimatization: Take a certain amount of bacterial liquid on the cultured biofilm and prepare a certain concentration of bacterial suspension. They were respectively placed in beef extract pept...

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Abstract

The present invention relates to the tolerance and adsorbability of microbe in biomembrane for treating low concentration lead-containing waste water, and discloses one kind of dominant bacterium, named as klebsiella pneumoniae CGMCC No.2016, shortly as Z-KAE15. Z-KAE15 has unheard-of high lead tolerance, high lead adsorbability and industrial practicability, and Z-KAE15 membrane can reach lead and COD removing rate of 95 % and 90 % separately. Z-KAE15 is obtained through the domestication on biomembrane in natural water, purifying, separating, repeated screening and 16s rDNA sequencing verification. The dominant bacterium Z-KAE15 is applied in environmental bioengineering to expand biomembrane process for continuous treatment of lead-containing sewage.

Description

technical field [0001] The invention belongs to the technical field of environmental bioengineering, and in particular relates to a dominant bacterium with high tolerance and high adsorption to lead, named Klebsiella pneumoniae. Preservation Unit: General Microbiology Center (CGMCC), China Committee for Culture Collection of Microorganisms, Address: Institute of Microbiology, Chinese Academy of Sciences, No. 13, North Yiyi Road, Zhongguancun, Haidian District, Beijing. Registration number of the preservation center: 2016, preservation date: 2007-04-23, referred to as Z-KAE15, is obtained by domestication, screening, purification and isolation using natural water biofilm. The bacterium can be used for the treatment of lead-containing wastewater and lead-containing substances. Background technique [0002] Lead mainly enters natural water bodies through atmospheric deposition, soil leachate, and surface runoff, and its sources are mainly mining metallurgy, electroplating, coa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F1/62C02F3/12C12Q1/68C12R1/22
CPCY02W10/10
Inventor 赵玉清张万筠陈吉群
Owner DALIAN NATIONALITIES UNIVERSITY
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