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Breeding method of oil-bearing crop with high content of vitamin A having functions of antisenescence, disease-resistant and oxidation resistance

A technology of oil crops and breeding methods, which is applied in the field of breeding of new varieties of oil crops, can solve problems such as not proposing research purposes and solving problems, and achieve the effects of high economic use value, prolonged dormancy time, and accurate results

Inactive Publication Date: 2008-03-12
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology

At home, the inventor of this patent has established a rapid measurement technology of rapeseed carotenoid content (Gao Guizhen, etc., research on the method for measuring the total amount of rapeseed carotenoids, Journal of Plant Genetics, 2005, 6: 414-417), Started the screening of rape variety resources, but did not put forward a clear research purpose and a technical solution to the problem

Method used

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  • Breeding method of oil-bearing crop with high content of vitamin A having functions of antisenescence, disease-resistant and oxidation resistance
  • Breeding method of oil-bearing crop with high content of vitamin A having functions of antisenescence, disease-resistant and oxidation resistance
  • Breeding method of oil-bearing crop with high content of vitamin A having functions of antisenescence, disease-resistant and oxidation resistance

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Embodiment Construction

[0035] The method of the present invention is as follows: 1) use spectrophotometry to screen a large number of germplasm resources, or use genetic engineering technology to overexpress key genes for carotenoid synthesis, and obtain seeds with a carotenoid content higher than 60 μg gFW -1 2) Using the basic germplasm with high carotenoids as the donor and the fine variety as the recipient, use conventional breeding techniques to carry out hybridization and transfection to obtain high-carotenoid high-quality varieties; 3) Use HPLC method to determine the selection and breeding Seed carotenoid content of varieties, control it at 60-300μg gFW -1 within range.

[0036] Oil crops are rapeseed, soybean, peanut, sesame, sunflower, safflower, flax and corn.

[0037]In order to understand the present invention better, take rape as an example below, further set forth content of the present invention:

[0038] With reference to Fig. 1, concrete steps of the present invention are:

[00...

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Abstract

A cultivation method of oil plant, which contains rich vitamin A and is anti-aging, disease resistant and oxidation resistant relates to the oil plant breeding technology. The method is that the spectrophotometer is used for selecting large amounts of seed resource or the gene engineering technology is used for over-expressing the crucial genes of synthesizing the carotenoid; the basic germ plasm of seeds selected contain the carotenoid which is higher than 60Mu ggFW-1; the basic germ plasm with high carotenoid content is taken as the donor and the high-quality seeds are taken as the acceptor; the conventional breeding technology is used for hybridizing to obtain high-quality breeds with high carotenoid; the HPLC method is used for detecting the carotenoid content of the breeding seeds to control the content in the range of 60-300Mu ggFW-1. The selecting method of the present invention is simple with high efficiency, accurate results, low cost and reliable resources. The plant oil squeezed from the oil plant cultivated with the method of the present invention contains rich vitamin A and is characterized by high nutrition and anti-aging, disease resistant and deterioration resistant functions.

Description

technical field [0001] The invention relates to the technical field of breeding of new varieties of oil crops. Background technique [0002] Carotenoids are a class of pigments derived from isoprenoids that are widely found in plants and other organisms. In plants, carotenoids are an important part of the photosynthetic system, assisting light energy absorption and having antioxidant effects. At the same time, carotenoids are also essential for human nutrition and health. Existing studies have shown that carotenoids can have the following functions: ① carotenoids, especially β-carotene, are the precursors of vitamin A (ProvitaminA, provitamin A), and vitamin A is an essential micronutrient for the human body; ② It has immune function, which can improve the ability of the human immune system to resist pathogens; ③ It has the function of antioxidant, which can quench the adverse effects of singlet oxygen and remove free radicals in the body, thereby delaying human aging; ④ C...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04C12N15/82G01N21/00C07C403/24
Inventor 伍晓明高桂珍陆光远陈碧云许鲲李响枝
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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