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Application of 5,6,7-trihydroxy flavone in the preparing of medicine for restraining bacterium

A trihydroxyflavonoid and antibacterial technology, applied in the field of microorganisms, can solve problems that do not involve the inhibition mechanism of the quorum sensing system

Inactive Publication Date: 2008-03-12
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pharmacological effects reported in the literature include the following aspects, but none of them involve its inhibitory mechanism on the quorum sensing system

Method used

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  • Application of 5,6,7-trihydroxy flavone in the preparing of medicine for restraining bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Virtual screening experiment of molecular docking software DOCK

[0032] PDB introduction: PDB is a free protein-related database, the web link is: http: / / www.pdb.org / pdb / home / home.do .

[0033] Introduction of TraR protein: TraR protein belongs to the LuxR family protein and is the receptor protein of the quorum sensing system of Agrobacterium. When it binds to its signaling molecule (3-oxo-c8-HSL), it will change its own conformation. Become a transcriptional activator of quorum sensing-related genes and promote the expression of related genes.

[0034] Molecular docking software DOCK: DOCK software is a molecular docking software developed by the research group of Professor Kuntz, Department of Pharmaceutical Chemistry, University of California, San Francisco. The principle of the software is to dock the compound in the database with the active site of the receptor to obtain a complex with electrostatic force and stereoscopic factors. The best way to re...

Embodiment 2

[0038] Embodiment two: 5,6,7-trihydroxyflavone inhibits the experiment of Pseudomonas aeruginosa biofilm formation

[0039] Add 975 μl of overnight cultured Pseudomonas aeruginosa bacteria solution, 25 μl of 40% glycerol, and 1 μl of 200 mM 5,6,7-trihydroxyflavone into a 2 ml plastic centrifuge tube. The treatment without 5,6,7-trihydroxyflavone was used as negative control. Cultured at 37°C for 3 days. Carefully pour off the liquid in the centrifuge tube, and gently rinse the centrifuge tube several times with sterilized distilled water to ensure that free bacteria in the centrifuge tube are washed away. After the water droplets on the inner wall of the centrifuge tube evaporate, add 1ml of 0.5% crystal violet solution and let stand at room temperature for 30 minutes. Pour off the crystal violet, and gently rinse the centrifuge tube several times with sterilized distilled water to ensure that the crystal violet not adsorbed on the biofilm is washed away. After the water dr...

Embodiment 3

[0041] Example three: 5,6,7-trihydroxyflavone inhibits Vibrio harveyi biofilm formation experiment

[0042] Add 975 μl of overnight-cultured Vibrio harvelii liquid, 25 μl of 40% glycerol, and 1 μl of 200 mM 5,6,7-trihydroxyflavone into a 2 ml plastic centrifuge tube. The treatment without 5,6,7-trihydroxyflavone was used as negative control. Cultured at 37°C for 3 days. Carefully pour off the liquid in the centrifuge tube, and gently rinse the centrifuge tube several times with sterilized distilled water to ensure that free bacteria in the centrifuge tube are washed away. After the water droplets on the inner wall of the centrifuge tube evaporate, add 1ml of 0.5% crystal violet solution and let stand at room temperature for 30 minutes. Pour off the crystal violet, and gently rinse the centrifuge tube several times with sterilized distilled water to ensure that the crystal violet not adsorbed on the biofilm is washed away. After the water droplets on the inner wall of the ce...

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Abstract

The present invention belongs to the micro biological field, which relates to the new application of a trihydroxyflavone of the small molecular compounds. The present invention provides the application of the trihydroxyflavone as a medicine for inhabiting the bacteria. The present invention firstly proves that the trihydroxyflavone has the active property of inhabiting the bacterial quorum-sensing. The present invention can be applied in the treatment of the microorganism infection of the human or animal in vitro or in vivo. The present invention can be also applied in prevention and treatment of the sterilization in the cultured water or the bacteria of the plants.

Description

technical field [0001] The invention belongs to the field of microorganisms and relates to a new application of a small molecular compound 5,6,7-trihydroxyflavone. Background technique [0002] With the abuse of antibiotics, the drug resistance of pathogenic bacteria is getting higher and higher, and the emergence of drug-resistant pathogens (such as Pseudomonas aeruginosa, Enterobacter, Vibrio, Legionella, etc.) makes it difficult to find therapeutic drugs for bacterial infections , human health is facing major challenges. [0003] Human or animal pathogenic bacteria such as Pseudomonas aeruginosa and Vibrio harveyi regulate their group behavior through the quorum sensing system, which includes the formation of biofilm and the expression of pathogenic factors. Quorum sensing refers to the mechanism by which bacteria regulate gene expression and coordinate group behavior according to changes in population density. The quorum sensing system of Gram-negative bacteria consist...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P31/04
Inventor 周世宁曾芝瑞钱力郑康成
Owner SUN YAT SEN UNIV
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