Sperm analyse

A semen, sperm technology, applied in the field of semen analysis, can solve the problem of excess light absorption rate measurement and other problems

Inactive Publication Date: 2008-03-12
M E S MEDICAL ELECTRONICS SYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This fact makes light absorbance measurements redu

Method used

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Examples

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example 1

[0088] As mentioned above, compared with animal samples, the automated optical measurement of TSC in human semen samples has been restricted in the past due to the lower concentration of sperm cells. This, together with the high electronic and optical background noise caused by, for example, the variability of the semen slurry, prevents the application of methods routinely used in veterinary student fertility analysis. The method of the present invention overcomes these obstacles by combining the following features:

[0089] (i) Place the sample in a transparent container between the synchronized pulsed light source and the synchronized activated photodetector. The use of a synchronized pulsed light source and a photodetector makes it possible to identify sperm cells at low concentrations at the level of electronic noise.

[0090] (ii) Measure the light absorption of the sample in the 800-1000nm range. It has been found that measuring absorbance in the near-infrared region provide...

example 2

[0096] Figure 2 shows an embodiment of a sampling device 20 according to the invention for use in measuring semen. The device includes an optical observation part 22 at the front, a suction part 24 at the rear, and an air discharge part 26 in the middle.

[0097] The optical observation section 22 includes a fine measurement chamber 28 and a coarse measurement chamber 30. The fine measurement chamber is used for measuring MSC and / or for visual inspection, and the coarse measurement chamber is used for measuring TSC. In this way, multiple parameters can be measured simultaneously using the same sampling equipment and sampling steps.

[0098] The suction part 24 includes a suction cylinder 32 and a plunger 34 slidably inserted therein. These components cooperate with each other and work as in a standard syringe. This part is used to suck the semen sample into the measurement chamber.

[0099] The air discharge part 26 includes a partition valve 36 for separating the measurement cham...

example 3

[0111] As described above, the determination of the MSC according to the present invention requires generating a voltage signal proportional to the MSC. Figure 5 shows an embodiment of a system for semen analysis that can generate such a signal.

[0112] The optical capillary 100 having a rectangular cross-section is used to hold the semen sample 102. The capillary 100 is illuminated by the incident light beam 105 generated by the light source 110. The capillary 100 has an optical path of 300 μm through which the light beam 105 passes. After passing through the capillary, the scattered beam 106 is collimated by a circular hole 108 with a diameter of 70 μm. The collimated light beam 107 irradiates the photodetector 115. The photodetector 115 generates an analog voltage signal 120 proportional to the intensity of the light beam 107. Due to the movement of the sperm in the semen sample 102, the analog signal changes with time, as illustrated in FIG. 8 by way of example. The analog si...

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Abstract

A method to measure total sperm concentration (TSC) in a semen sample comprises procedures below: (i) The semen sample is positioned in a transparent container between a synchronous impulse light source and a photodetector; (ii) Light absorbance of the semen sample in the range of 800 to 1,000mm is measured. TSC of the sample is directly proportional to the light absorbance. In addition, a sampling equipment to conduct optical analysis to biological body fluid, a method to measure motile sperm concentration (MSC), a method to confirm average speed (AV) of sperm cells and a system to detect semen quality. Wherein, the system is composed of a device to measure TSC, a device to measure MSC and a video visual examination system.

Description

[0001] This application is a divisional application of the invention patent application with the application number 01823286.8 (PCT / IL01 / 00475), the filing date of May 24, 2001, and the invention title "Semen Analysis". Technical field [0002] The present invention relates to semen analysis. Background technique [0003] According to WHO statistics, 8-10% of all married couples will consult medical experts after pregnancy failure. More than 40,000,000 couples are currently undergoing treatment for infertility. Among these infertile couples, it is estimated that 40% of these couples’ infertility is caused by male inducements, and another 20% is caused by a combination of male and female inducements. Semen analysis is a major technique in evaluating male triggers. [0004] Standard semen analysis procedures involve the determination of at least three main semen parameters: [0005] 1. Total sperm concentration (TSC); [0006] 2. Percentage of swimming sperm; and [0007] 3. Percent...

Claims

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Application Information

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IPC IPC(8): G01N21/59G01N33/487B01L3/00G01N15/06G01N21/03
CPCG01N21/359G01N33/48B01L3/502B01L2400/0478G01N15/06G01N21/03G01N21/11G01N2015/0003G01N2015/0053G01N2015/0065G01N2015/0693
Inventor 阿贝·基斯勒弗列夫·拉比诺维奇
Owner M E S MEDICAL ELECTRONICS SYST
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