Streptomycete antimicrobial production and method of preparing the same

Abstract

The invention relates to a streptomyces antibacteria product and a method for manufacturing the same, belonging to the biotechnology field. GB-2 seeds of the streptomyces are proliferated and cultivated, then inoculated to undergo submerged ferment under 28 DEG C for 144-168 hours, then the fermentation liquor is filtrated, n-hexane and ethyl acetate is used for twice extractions to eliminate the lipid fat-soluble impurities, and then an ultrafiltration membrane is used to intercept the biological molecules with the molecular weight below 1,000, the filter liquor is made pass through a 1-2nm nanometer filtration membrane to obtain the antibacterial metabolic product. As proved by test, the antibacterial substance has the broad-spectrum antibacterial activity against bacteria and epiphyte, as well as the significant antibacterial activity against multiple pathogenic organisms and spoilage organisms. The antibacterial product provided by the invention has significant inhibitive effect on multiple pathogenic bacterium and moulds, thereby manifesting the efficient and broad-spectrum activity, simple method and low cost, with high applicable value and great prospect in the market in the respect of antiseptics, forage additives and medicine.

Description

1. Technical field [0001] The invention relates to a Streptomyces antibacterial product and a production method thereof, belonging to the field of biotechnology. It can be used in food preservatives, cosmetic preservatives, feed additives, etc. 2. Technical background [0002] In recent years, due to the increasingly serious problems of drug abuse, drug residues and bacterial resistance, people have paid attention to food safety and drug safety. It is urgent to find new and more effective antibacterial substances, which has become a research hotspot all over the world. [0003] In the past more than half a century, people have mainly developed and obtained new antibacterial substances from the surfaces of animals and plants growing in different environments, such as cold zone, temperate zone, tropical zone, crater and glacier, as well as on the surface of animals and plants in different regions. However, with the continuous deepening of research and the increasing urgency ...

AI Technical Summary

Problems solved by technology

In terms of preservatives, the only natural preservatives allowed by my country's food additive standards (GB2760) are Nisin and Natamycin, among which Natamycin comes from Streptomyces natal in actinomycetes (Streptomyces natalensis), a polyene macrolide compound, is a highly effective fungal inhibitor that can effectively kill mold, yeast and filamentous fungi, but is ineffective against bacteria and viruses