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Method for producing single cell protein feed using waste molasses

A single-cell protein and waste molasses technology, which is applied in the field of waste resource utilization to achieve the effects of vigorous bacterial metabolism, obvious economic benefits and low price.

Inactive Publication Date: 2008-04-16
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are still few manufacturers using waste molasses fermentation to produce single-cell protein, and there are no reports about using mixed strains to ferment waste molasses to produce single-cell protein feed.

Method used

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  • Method for producing single cell protein feed using waste molasses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Dilute the waste molasses with water to a concentration of 20g / 100ml, and the sag after dilution reaches 20% (Brix: 20%); then adjust the pH of the waste molasses to 5 with dilute hydrochloric acid (hydrochloric acid and water are mixed in a volume ratio of 1:9) , let it stand for 12 hours, take the supernatant night; add yeast extract 3g / 100ml, magnesium sulfate 0.3g / 100ml, superphosphate 0.6g / 100ml, potassium dihydrogen phosphate 0.3g / 100ml, mix and sterilize evenly, and then throw in Geotrichum candidum The mixed bacteria composed of GIM2.69, Candida tropicalis GIM2.7 and Candida utilis GIM2.8, the weight percentage of the three kinds of bacteria is 1:1:1, and the insertion amount of bacteria is 10%. Under the condition of oxygen, the yeast was cultured in a constant temperature shaking incubator at 100r / min at 30°C for 72 hours, and then the yeast cells were centrifuged and washed (see Figure 1 for the cleaning method of the cells), and then the yeast cells were Dry...

Embodiment 2

[0012] Dilute the waste molasses with water to a concentration of 15g / 100ml, and the sag after dilution reaches 20% (Brix: 20%); then adjust the pH of the waste molasses to 4.5 with dilute hydrochloric acid (hydrochloric acid and water are mixed in a volume ratio of 1:9) , stand still for 12 hours, take the supernatant night; add urea 3g / 100ml, magnesium sulfate 0.2g / 100ml, superphosphate 0.3g / 100ml, potassium dihydrogen phosphate 0.2g / 100ml, mix and sterilize evenly, and then throw in Geotrichum candidum GIM2 .69, the mixed bacteria composed of Candida tropicalis GIM2.7 and Candida utilis GIM2.8, the weight percentage of the three kinds of bacteria is 1:1:1, the insertion amount of bacteria is 15%, in aerobic Under conditions, yeast culture was carried out in a constant temperature shaking incubator at 100r / min at 28°C for 60 hours, and then the yeast cells were centrifuged and washed (see Figure 1 for the cleaning method of the cells), and then the yeast cells were washed at ...

Embodiment 3

[0014] Dilute the waste molasses with water to a concentration of 20g / 100ml, and the sag after dilution reaches 20% (Brix: 20%); then adjust the pH of the waste molasses to 5.5 with dilute hydrochloric acid (hydrochloric acid and water are mixed in a volume ratio of 1:9) , let it stand for 12 hours, take the supernatant night; add sodium nitrate 3g / 100ml, magnesium sulfate 0.4g / 100ml, superphosphate 0.5g / 100ml, potassium dihydrogen phosphate 0.4g / 100ml, mix and sterilize evenly, and then throw in Geotrichum candidum The mixed bacteria composed of GIM2.69, Candida tropicalis GIM2.7 and Candida utilis GIM2.8, the weight percentage of the three kinds of bacteria is 1:1:1, and the insertion amount of bacteria is 18%. Under the condition of oxygen, the yeast was cultured in a constant temperature shaking incubator at 100r / min at 32°C for 50 hours, and then the yeast cells were centrifuged and washed (see Figure 1 for the cleaning method of the cells), and then the yeast cells were ...

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Abstract

The present invention discloses a method for generating single cell albumen feedingstuff using waste gooey. To dilute the waste gooey until its chroma is 10 to 25 g / 100ml and back sagis is about 20 percent by adding water; then to adjust the pH value of the gooey to 4.0 to 6.0 by using rare hydrochloric acid, and placing for 12 hours so as to fetch upper clear liquid; adding nitrogen source with 0.00 to 10g / 100ml, phosphate with 0.5 to 1.5g / 100ml and magnesium salt with 0.1 to 0.5g / 100ml separately; adding the gooey culture medium after destroying bacterium into a mixing bacterium seed composing of geotrichum mildew, tropical candida and prion-producing candida, the weight ratio of the three bacteriums is 1:1:1, the access amount of the bacterium is 5 to 20 percent, under the condition of good oxygen, to bring with 44 to 72 hours at the temperature of 25 to 35 DEG C, then to separate the microzyme body, after cleaning the bacteriums to dry at the temperature of 105 DEG C to obtain single cell albumen feedingstuff. The present invention has low cost, composing method is simple, bacterium metabolizing is bloom, producing ratio is high, and it is not abstrained by area as well as climate condition.

Description

technical field [0001] The invention relates to the application field of waste resources, in particular to a method for producing single-cell protein feed by using microorganisms to ferment waste molasses. Background technique [0002] At present, there are many examples of resource utilization of waste molasses at home and abroad. Most of the researches on the resource utilization of molasses, a by-product of sugar factories, use molasses as a raw material to ferment ethanol. There are also many manufacturers using waste honey to prepare citric acid, L-lysine and glacial acetic acid, etc., and some manufacturers specifically extract caramel pigment from them, and some directly use molasses as feed additives to feed poultry and livestock. However, in my country, especially in Guangxi Province, which is the main sugar production area, waste molasses is mainly used for fermentation to produce alcohol, but the profit value of alcohol production is low, and the fermentation wast...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/02A23K10/33
CPCY02P60/87
Inventor 曾鸿鹄姚毅
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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