Application of knotting thread for filamentous fungi breeding and breeding method for filamentous fungi

A technology of filamentous fungi and silk thread, which is applied in the field of propagation of filamentous fungi using silk thread, can solve the problems of unsustainable operation and reduction of reactor biomass, and achieve the effects of low cost, large reproduction and growth, and easy popularization and application

Inactive Publication Date: 2008-04-23
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] One of the purposes of the present invention is to solve the problem that the filamentous fungus reactor cannot continue to operate due to the reduction of biomass, and provides a use of silk as a carrier, that is, a method of using silk as a carrier to promote the growth of filamentous fungi to maintain the growth of filamentous fungi in the reactor. Filamentous fungal biomass

Method used

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  • Application of knotting thread for filamentous fungi breeding and breeding method for filamentous fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Production of knotted cotton thread

[0036] Take a piece of cotton thread and fold it in half, and tie a knot at one end of the folded cotton thread, the purpose of which is to keep the carrier in a certain shape during utilization; cut it off at a distance of 80 mm from the knot; untie the cotton thread wrapped around both ends of the knot, Let the thin threads that make up the cotton thread not be entangled with each other. Put the carrier in a pot and boil for 30 minutes, wash it with deionized water for 3 times, and boil it continuously for 3 times to remove impurities on the cotton thread, and dry the boiled knotted cotton thread to obtain a knotted cotton thread (see Figure 1).

[0037] (2) The Bonnarme medium of 300ml (Bonnarme medium formula: glucose 10g / L, ammonium tartrate 0.8g / L, KH 2 PO 4 2g / L, MgSO 4 0.5g / L, CaCl 20.1g / L, yeast extract powder 0.2g / L, 20mmol / L acetic acid: sodium acetate buffer (pH=4.4), 70ml trace elements (containing: MgSO 4 ...

Embodiment 2

[0039] Experiments on Propagation and Growth of Filamentous Fungus by Knotted Cotton Carrier

[0040] Add 0, 40, 60, and 80 knotted cotton thread carriers to 100ml Bonnarme medium, and sterilize for 30 minutes at 121°C; then inoculate Phanerochaete chrysosporium spores, and inoculate 10 4 spores, and then placed at 37°C, cultured on a shaker at 160rpm for 5 days; the knotted cotton thread carrier for growing mycelium was taken out, then washed with deionized water, and dried at 105°C, and then the mycelium attached to it was measured (Measurement method: the difference obtained by subtracting the weight of the carrier without bacteria from the weight of the carrier with bacteria attached is the biomass of the attached growth hyphae). Results The average biomass of mycelia cultured without carrier was 0.2899g, while the biomass of mycelia grown with 40, 60 and 80 carriers were 0.4947g, 0.5139g and 0.5557g respectively. It shows that the knotted cotton thread carrier can promot...

Embodiment 3

[0042] Knotted Cotton Carrier Promotes Production of Manganese Peroxidase in White Rot Fungus

[0043] In 100ml Bonnarme culture medium, set not to add knotted cotton thread and to add knotted cotton thread carrier 80 two kinds of treatments, under the condition of 121 ℃, sterilize for 30 minutes; Then inoculate Phanerochaete chrysosporium spore (10 4 spores) were cultured at 37°C on a shaker at 160 rpm; manganese peroxidase was detected every day from the second day (Paszczynski method——Paszczynski A, Crawford RL, Huynh V B. Manganese peroxidase of Phanerochaete chrysosporium: purification.Methods in Enzymology, 1988, 161(2): 264-270. Definition of oxidation 1mmol / min Mn 2+ for Mn 3+ The required enzyme amount is 1 enzyme activity unit); As a result, in the culture without carrier, manganese peroxidase began to appear enzyme activity on the 4th day, and the highest enzyme activity could reach 535U / L; while adding 80 In the culture of two carriers, the enzyme activity began ...

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Abstract

The present invention discloses use of knotted silk thread in propagating filamentous fungi, and the process of propagating filamentous fungi on knotted silk thread carrier. The knotted silk thread may be sunk into water and stretched in water for hypha to attach on easily; and the knotted silk thread is flexible for hypha to be broken and propagated periodically. The knotted silk thread has facile material and excellent use effect, and is favorable to propagation and growth of filamentous fungi.

Description

technical field [0001] The invention belongs to the field of industrial microbes, and in particular relates to the application of silk threads in the propagation of filamentous fungi and a method for the propagation of filamentous fungi using silk threads. Background technique [0002] Refractory organic pollutants are decomposed by microorganisms very slowly and incompletely, and remain in the environment for a long time, causing serious environmental pollution. Studies have found that white rot fungi can degrade a variety of refractory organic pollutants, and thus become an effective way to treat refractory pollutants. However, the continuous operation of white-rot fungal reactors under non-sterile conditions has not been realized so far, and the main reason is that the biomass of white-rot fungi in the reactor cannot be maintained for a long time. [0003] There are two main ways to maintain the biomass of white rot fungi: one is the unlimited growth of mycelia; the othe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N11/00
Inventor 文湘华周成
Owner TSINGHUA UNIV
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