Method for preparing high-purity beta-carotene from green tea

A carotene and green tea technology, applied in the field of extraction and purification of β-carotene, can solve the waste of biological resources and other problems, and achieve the effects of simple equipment, expanded development and utilization prospects, and low production costs

Inactive Publication Date: 2008-05-21
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large amount of spring tea leaves and summer and autumn tea leaves are not fully utilized, and a small part is processed into low-grade green tea and sold at a low price. Farmers are unwilling to pick summer and autumn tea, resulting in a waste of a large amount of biological resources.

Method used

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  • Method for preparing high-purity beta-carotene from green tea

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Experimental program
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Effect test

Embodiment 1

[0017] (1) Take the low-grade green tea slices collected in summer from the tea production area in Hangzhou, Zhejiang, and grind them mechanically to 200 mesh at room temperature. The green tea powder was extracted with 60% aqueous ethanol (g / ml, 1:10) at 25° C. for 5.0 hours and stirred (stirring speed: 150 r / min). Let stand, filter, and dry the filter residue under reduced pressure;

[0018] (2) The filter residue in step (1) was extracted with n-hexane (g / ml, 1:12) at 28°C for 6 hours, stirred (stirring speed: 200r / min), left standing, filtered, and the filtrate was freeze-dried Concentrate to 1 / 3 of the original volume to obtain crude extract of green tea β-carotene;

[0019] (3) The green tea β-carotene crude extract obtained in step (2) is subjected to silica gel column chromatography, the eluent is ethyl acetate and n-hexane, and the elution gradient sequence is: 1.0:9.0; 2.0:8.0; 3.0:7.0; 3.5: 6.5 (ethyl acetate: n-hexane, V: V), the flow rate is 15ml / min, the efflue...

Embodiment 2

[0021] (1) Take the low-grade green tea flakes collected in autumn from the Kaihua tea production area in Zhejiang, and grind them mechanically to 300 mesh at room temperature. The green tea powder was extracted with a mixed solution containing ethanol, methanol and water (1:1:0.8) at 18° C. for 2.5 hours and stirred (stirring speed: 150 r / min). Let it stand, filter, and dry the filter residue under reduced pressure. The mass volume ratio (g / ml) of green tea powder and above-mentioned extracting solution is 1: 7.5;

[0022] (2) The filter residue in step (1) was extracted with n-hexane and acetone (1:1) at 22°C for 3 hours, stirred (stirring speed: 200r / min), allowed to stand, filtered, and the filtrate was freeze-dried and concentrated to 1 / 3 of the original volume to obtain crude extract of green tea β-carotene;

[0023] (3) Aluminum oxide column chromatography on the green tea β-carotene crude extract obtained in step (2), the eluent is chloroform and isohexane, and the e...

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Abstract

A method for preparing high-purity β-carotene from green tea. The main contents are as follows: first extract the pulverized green tea powder with a mixture of methanol, ethanol and H2O or at least one of them, and dry the extracted tea dregs under reduced pressure, and then extract them with ethyl acetate, acetone, n-hexane, isohexane, One or more of dichloromethane and trichloromethane are used as the extracting solution to extract the crude extract of green tea β-carotene. After the crude extract is concentrated, it is subjected to gradient elution chromatography on silica gel or alumina column chromatography. The eluents are two of ethyl acetate, acetone, n-hexane, isohexane, cyclohexane, dichloromethane and chloroform. The effluent is collected in parts and vacuum freeze-dried to obtain high-purity green tea β-carotene. The invention is suitable for common green tea, especially low-grade green tea as raw material, and has the characteristics of simple technological process and required equipment, high extraction rate and high purity of β-carotene in tea leaves.

Description

technical field [0001] The invention belongs to the deep processing technology of green tea, and relates to a method for extracting and purifying β-carotene from green tea. Background technique [0002] Tea, as a drink with both flavor characteristics and nutritional and health functions, has existed in my country for thousands of years. It is an important economic crop and an agricultural product that earns foreign exchange in my country. At present, the world tea industry is facing contradictions such as the growth rate of tea production is faster than consumption, and the export price of tea (especially bulk green tea) has fallen. The degree of correlation can promote the sustainable development of my country's tea industry. The extraction and utilization of functional components in the field of tea deep processing is an important way for the comprehensive utilization of tea resources. At present, it mainly focuses on substances such as tea polyphenols, theanine, theaflav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24A61K36/82
Inventor 陈小强叶阳成浩
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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