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Cultivating method for high-yield itaconic acid strain

A cultivation method, itaconic acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of slow progress in research work, reduce residual sugar content, improve utilization rate, and produce acid rate-enhancing effect

Inactive Publication Date: 2008-07-09
QINGDAO LANGYATAI GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since 1985 in our country, many scientific research units have carried out long-term research on the process and strains of itaconic acid produced by fermentation, but the progress of the research work has been slow due to the lack of ideal strains.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: with 300m 3 Fermentation tanks to produce itaconic acid The strains isolated from fermenters whose acid value reaches 7.5g / 100ml are the starting strains. 6 h 12 o 6 ·H 2 O content is 1.5%, agar content is 1%, inorganic salt MgSO 4 ·7H 2 O content is 0.25%, (NH 4 ) 2 SO 4 Content is 0.3%, K 2 HPO 4 Content is 0.02%, FeSO 4 After culturing in a medium with a content of 0.002%, take the slant with normal growth, uniform hyphae, and plump spores as the object of separation and purification. After being purified on a plate, take 10 -3 The bromocresol green bacterial species screening medium plate was cultured and spread on the dilution, and after being irradiated vertically by a 20W ultraviolet lamp at a distance of 20cm for 20 seconds, it was quickly moved into a constant temperature incubator, and cultivated for 3 days at 30°C with a relative humidity of 20%. When a small amount of gray-brown spores appear in the center of the colony, select the colo...

Embodiment 2

[0013] Example 2: with 300m 3 Fermentation tanks ferment to produce itaconic acid The strains isolated from fermenters whose acid value reaches 7.0g / 100ml are the starting strains. 6 h 12 o 6 ·H 2 O content is 1.5%, agar content is 2%, inorganic salt MgSO 4 ·7H 2 O content is 0.5%, (NH 4 ) 2 SO 4 Content is 0.3%, K 2 HPO 4 Content is 0.01%, FeSO 4 After culturing in a medium with a content of 0.003%, take the slant with normal growth, uniform hyphae, and plump spores as the object of separation and purification. After being purified on a plate, take 10 -4 The bromocresol green bacterial species screening medium plate was cultured and spread on the dilution, and after being irradiated vertically by a 40W ultraviolet lamp at a distance of 30cm for 30 seconds, it was quickly moved into a constant temperature incubator, and cultivated for 4 days at 40°C with a relative humidity of 30%. When a small amount of gray-brown spores appear in the center of the colony, select t...

Embodiment 3

[0016] Example 3: with 300m 3 Fermentation tank fermented to produce itaconic acid The strain isolated from the fermenter whose acid value reached 7.2g / 100ml was the starting strain. 6 h 12 o 6 ·H 2 O content is 2%, agar content is 1.5%, inorganic salt MgSO 4 ·7H 2 The O content is 0.6%, (NH 4 ) 2 SO 4 Content is 0.5%, K 2 HPO 4 Content is 0.01%, FeSO 4 After culturing in a medium with a content of 0.003%, take the slant with normal growth, uniform hyphae, and plump spores as the object of separation and purification. After being purified on a plate, take 10-5 The bromocresol green strain selection medium plate was cultured and spread on the dilution, and after being irradiated vertically by a 50W ultraviolet lamp at a distance of 40cm for 40 seconds, it was quickly moved into a constant temperature incubator, and cultivated for 5 days at 50°C with a relative humidity of 40%. When a small amount of gray-brown spores appear in the center of the colony, select the colo...

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PUM

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Abstract

The invention discloses a method for breeding strain with highly yield itaconic acid, wherein selecting strain with producing itaconic acid as depart strain, which is cultured in an incubator after culturing, separation and purification, selecting bacterial colony which has large color-changing loop, wherein the spores are picked on a test tube inclined-plane for culturing to obtain mature spores which have high-yield itaconic acid via shaking table. When producing itaconic acid which is produced by the strain utilizing the method, saccharic acid percent conversion is capable of achieving 65%, acid production via invoice method is capable of achieving 9.01g / 100ml, improving productivity of the itaconic acid, reducing residual sugar content of fermentation liquor, thereby facilitating the processing of subsequent abstraction process such as filtration of the fermentation liquor and the like, simultaneity, improvement of acid production ratio also increases availability ratio of raw materials, thereby reducing cost of production.

Description

technical field [0001] The invention relates to a method for cultivating microorganisms. Background technique [0002] Itaconic acid is a dibasic unsaturated organic acid with a wide range of uses, which rarely exists in nature. In 1837, itaconic acid was isolated from citric acid pyrolysis products for the first time in the world. After 1929, researchers from various countries discovered that Aspergillus terreus could ferment glucose to produce itaconic acid, and since then began the research history of itaconic acid prepared by fermentation. Since 1985 in our country, many scientific research units have carried out long-term research on the process and strains of itaconic acid produced by fermentation, but the progress of the research work has been slow because the ideal strains have not been obtained. [0003] At present, the general itaconic acid manufacturers usually screen and expand the culture of strains in the traditional way. Screening at various levels is mixed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/44C12R1/645
Inventor 李悦明张希铭徐建春夏修峦孙慧彬刘同照陈金凤曲文强
Owner QINGDAO LANGYATAI GRP
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